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FLASH GENE

Symbol

PRSS21

contributors: SGE - updated : 30-04-2010

HGNC name	protease, serine, 21 (testisin)
HGNC id	9485

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	16p13.3      Physical location : 2.867.163 - 2.871.716
Synonym name	serine protease from eosinophils
	testisin
	ESP-1, eosinophil serine protease 1
Synonym symbol(s)	TSTS, EPS1, TEST1, ESP1, TESTISIN
EC.number	3.4.21.-

DNA

TYPE	functioning gene
STRUCTURE	4.56 kb     6 Exon(s)

10 Kb 5' upstream gene genomic sequence study

regulatory sequence	Promoter (CAAT box)
	cytosine-phosphate-guanine/HTF
	Binding site   transcription factor
motif
text structure	a CpG island near a major initiation site
	Cpg sites in the 5'UTR are relevant for gene silencing when methylated in testicular germ cell tumors (Kempkensteffen 2006)

MAPPING

cloned

Y

linked

N

status

confirmed

Map	pter - D16S521 - HAGH - TPSAB1 - D16S3024 - CLCN7 - ZNF174 - D16S3070 - ATP6V0C - TSC2 TSC2 - PRSS21 - D16S510 - D16S3128 - D16S418 - cen

regionally located

close to D16S246

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_006799 6 - 1165 NP_006790 35 314 - 1998 9826525 precursor isoform 1 NM_144956 6 - 1159 NP_659205 - 312 - 1999 10397266 isoform 2 NM_144957 6 - 1135 NP_659206 - 300 - 1998 9826525 isoform 3

EXPRESSION

Type	restricted

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Endocrine pancreas         Lymphoid/Immune spleen         Reproductive female system uterus cervix       female system ovary         male system testis     highly   male system prostate       Respiratory lung           respiratory tract larynx       Urinary kidney

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Muscular striatum skeletal

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic eosinophil Reproductive spermatocyte

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	membrane type serine-proteinase
	a signal peptide
	a catalytic domain
	a C terminal hydrophobic extension (anchor)

conjugated

GlycoP , LipoP

HOMOLOGY

Homologene

FAMILY

trypsin family of serine proteases

CATEGORY

enzyme , tumor suppressor

SUBCELLULAR LOCALIZATION	plasma membrane
text	localized on the surface of cultured tumor cells as a glycosyl-phosphatidylinositol-linked protein
	localized at plasma membrane of premeiotic testicular germ cells

basic FUNCTION	regulating proteolytic events associated with testicular germ cell maturation
	may be involved in progression of testicular tumors of germ cell origin
	can promote cellular processes that drive malignant transformation
	may promote carcinogenesis by inhibiting tumor suppressor activity of SERPINB5 )
	may be active on peptide linkages involving the carboxyl gp of lysine or arginine
	proteolytic factor that directs epididymal sperm cell maturation and sperm-fertilizing ability

CELLULAR PROCESS	cell life, proliferation/growth

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

INTERACTION

DNA

RNA

small molecule

protein

interacting with SERPINB5

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in ovarian cancer tumoral     --low   in testicular tumors (role in progression of testicular tumor of germ cell origin)

Susceptibility

Variant & Polymorphism

Candidate gene	aberrant regulation of PRSS21 may underlie certain secondary male infertility syndrome, such as "easily decapited spermatozoa"
Marker
Therapy target
	System Type Disorder Pubmed cancer     target for therapeutic intervention in cancer

ANIMAL & CELL MODELS