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FLASH GENE

Symbol

VAMP3

contributors: mct - updated : 28-03-2018

HGNC name	vesicle-associated membrane protein 3 (cellubrevin)
HGNC id	12644

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

DNA

TYPE	functioning gene
STRUCTURE	10.16 kb     5 Exon(s)

Genomic sequence alignment details
10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_004781 5 - 2225 NP_004772 11.18 100 - 2009 19159614

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive esophagus       highly Urinary bladder       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Blood / Hematopoietic

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic platelet

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	an amphipathic helix which may participate in coiled coil interactions
	a C terminal hydrophobic domain predicted to serve as a membrane anchor

HOMOLOGY

Homologene

FAMILY

synaptobrevin family

CATEGORY

transport

SUBCELLULAR LOCALIZATION	plasma membrane
	intracellular
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,endosome
	intracellular,cytoplasm,cytosolic,vesicle
text	v-SNARE that resides in recycling endosomes and endosome-derived transport vesicles
	VAMP3 and VAMP7 colocalize with the major myelin proteolipid protein (PLP1) in recycling endosomes and late endosomes/lysosomes, respectively

basic FUNCTION	soluble N ethylmaleimide-sensitive factor-attachment protein receptor, SNARE protein
	associated with the early endosome, involved in regulating membrane traffic
	VAMP3 and AP1B1 cooperate in basolateral membrane trafficking
	implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration
	transport vesicles containing VAMP3 have distinct membrane fusion kinetics with domains of the plasma membrane that present different t-SNARE proteins
	SNARE protein that mediates exocytosis, in cell migration and integrin trafficking
	VAMP3-dependent integrin trafficking is crucial in cell migration and cell adhesion to laminin, and VAMP3-mediated integrin trafficking is critical in cell migration and cell adhesion to laminin
	with other VAMPs, has differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs
	involvement of VAMP3/cellubrevin and VAMP7/TI-VAMP in myelin membrane trafficking
	regulates podosome organisation in macrophages and together with STX4/SNAP23 mediates adhesion, cell spreading and persistent migration
	platelets are capable of a range of endocytosis steps, with VAMP3 being pivotal in these processes

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

cellular trafficking transport

text	vesicular trafficking

PATHWAY

metabolism

signaling

potential new integrin trafficking pathway in which endocytosed integrins are transported from VAMP3-containing recycling endosomes to STX6-containing trans-Golgi network before being recycled to the plasma membrane

a component	complex between proteins on vesicle and target membranes
	VAMP-3 and VAMP-8 forming SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion

INTERACTION

DNA

RNA

small molecule

protein	interacting with BCAP29
	with SNAP23 and STX12, have a role in the trafficking of MMP during degradation of ECM substrates and subsequent cellular invasion
	regulates podosome organisation in macrophages and together with STX4/SNAP23 mediates adhesion, cell spreading and persistent migration
	RNF130 ubiquitin ligases play a novel role in regulating recycling endosome trafficking via ubiquitylation of the VAMP3 SNARE protein
	PI4K2A is a binding partner of vesicle-associated membrane protein 3 (VAMP3), a small R-SNARE involved in recycling and retrograde transport, and the two proteins co-reside on tubulo-vesicular endosomes
	small GTPase RAB8A interacts with VAMP3 to regulate the delivery of recycling T-cell receptors to the immune synapse
	VAMP3 interacts with SLC12A1 and mediates its constitutive exocytic delivery to the apical surface, and VAMP3 is required for normal SLC12A1 expression, renal function, and blood pressure

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function constitutional     --other   silencing inhibits cell adhesion to laminin and effectively reduced cell motility constitutional     --over   protects cardiac glucose metabolism under conditions of impaired insulin sensitivity

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS

Vamp3-/- mice had less platelet-associated Fg, indicating a defect in Fg uptake/storage