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FLASH GENE
Symbol POU2AF1 contributors: mct - updated : 17-11-2015
HGNC name POU domain, class 2, associating factor 1
HGNC id 9211
EXPRESSION
Type widely
constitutive of
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestivemouthtongue  highly
Lymphoid/Immunelymph node   highly
 spleen   highly
 tonsils   highly
Reproductivemale systemprostate  moderately
Respiratorylung   moderately
Urinarykidney   moderately
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Blood / Hematopoietic    
Lymphoid    
cells
SystemCellPubmedSpeciesStageRna symbol
Lymphoid/ImmuneB cell
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • a N terminal domain critical for DNA binding and interaction with POU specific and POU homeo (helix-turn-helix) domains of OCTs
  • a C terminal domain mainly responsible for transactivation
  • mono polymer complex
    HOMOLOGY
    interspecies homolog to murine Pou2af1
    Homologene
    FAMILY
  • POU2AF1 family
  • CATEGORY immunity/defense , transcription factor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus
    basic FUNCTION
  • B-cell specific coactivator, involved in the response of B cells to antigens and required for the formation of germinal center and for normal transcription and v-(D)-J recombination of a subset of immunoglobulin Kappa genes
  • stabilizes POU dimer-DNA interactions
  • a clamp effect on the binding of POU2F1 monomer when it seems compromised
  • stimulates PORE activity
  • lymphoid-specific transcriptional coactivator that associates with the transcription factors POU2F1 or POU2F2 on the conserved octamer element present in the promoters of several ubiquitous and lymphoid-specific genes
  • transcriptional coactivator essential at several stages of B-cell development, critical for T helper cell function
  • regulates the balance of TH1 versus TH2 mediated immunity
  • novel role for POU2AF1 anf IKZF3 in controlling the transcription and nuclear organization of genes involved in pre-BCR function
  • B lymphocyte-specific transcription factor which coactivates POU2F1 and POU2F2 on B cell specific promoters
  • its tight regulation expression in early B cells is essential to allow efficient B lymphocyte differentiation
  • with POU2F2, are B-cell associated transcription factors expressed in a subset of patients with acute myeloid leukemia (AML)
  • POU2AF1 together with POU2F1/POU2F2, binds to octamer sites in promoters and enhancers to activate transcription of immunoglobulin (Ig) genes
  • B cell-specific transcriptional factor that coactivates octamer-binding transcriptional factors POU2F1, and POU2F2 on B cell-specific promoters
  • POU2F1 and POU2AF1 are selectively required for the in vivo generation of CD4(+) memory T cells
  • CELLULAR PROCESS nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS
    text transcription activating factor
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA binding
    RNA
    small molecule
    protein
  • specifically with POU2F1 (OTF1) and POU2F2,fixing the Oct protein to the DNA
  • SIAHs leading to the destabilisation and degradation of POU2AF1 by the proteosome pathway
  • SPIB is a direct target gene critically regulated by POU2AF1
  • important function in IGH 3prime enhancer function and strongly favor an enhancer mechanism involving looping and facilitated factor recruitment rather than a tracking mechanism
  • ZFPM1 expression is directly or indirectly dependent on the B cell-specific coactivator POU2AF1 and it is modulated during B cell development: expression is observed in early but not in late stages of B cell development
  • cell & other
    REGULATION
    activated by upregulated in B cells either by bacterial lipolysaccharide or CD40 ligand and IL4
    induced by costimulation in cells
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral fusion      
    with LAZ3/BCL6 in a B-cell leukemia cell line (Karpas 231)
    tumoral fusion      
    with BTG4 in t(11;13)(q23;q12) in chronic lymphocytic leukemia
    Susceptibility to biliary cirrhosis
    Variant & Polymorphism SNP
  • rs4938534 is significantly associated to biliary cirrhosis
  • Candidate gene
    Marker
  • may be helpful in the differential diagnosis of classical Hodgkin's lymphoma (cHL) and primary mediastinal B-cell lymphoma (PMBCL) (the highest positive predictive value for PMBCL were POU2AF1)
  • Therapy target
    ANIMAL & CELL MODELS
    Bob1(-/-) mice showed imbalanced immune responses, resulting in increased susceptibility to Leishmania major infection