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FLASH GENE

Symbol

CPSF1

contributors: mct - updated : 23-03-2020

HGNC name	cleavage and polyadenylation specific factor 1, 160kDa
HGNC id	2324

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Lymphoid/Immune thymus       highly Reproductive female system ovary       Respiratory respiratory tract larynx     highly

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a bipartite nuclear localization signal (NLS)
	a RNA binding CPSF-AC terminal domain

mono polymer

heteromer , tetramer , complex

HOMOLOGY

interspecies

ortholog to Drosophila p160

Homologene

FAMILY

CATEGORY

RNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleoplasm

basic FUNCTION	recognition step of the polyadenylation reaction 3' processing of mRNA precursors
	CPSF1 functions as an essential scaffold and preorganizes CPSF4 and WDR33 for high-affinity binding to AAUAAA
	may play an important role in the development of retinal ganglion cell (RGC) axon projection
	both CPSF4 and WDR33 are required for high-affinity binding of the AAUAAA polyadenylation signal (PAS) RNA, while these two proteins alone and their binary complexes with CPSF1 have much lower affinity for the RNA
	mediates retinal vascular dysfunction by suppressing the phosphorylation mechanism in the mitogen-activated protein kinase/extracellular-signal-regulated kinase (MAPK/ERK) pathway in Diabetic retinopathy

CELLULAR PROCESS

nucleotide, transcription, maturation

PHYSIOLOGICAL PROCESS

text	polyadenylation

PATHWAY

metabolism

signaling

a component	complexing with CPSF2,3,4,poly (A) ribonuclease (PARN) and CSTF3 (cleavage stimulation factor 3)
	multisubunit complex that binds to the canonical AAUAAA hexamer and to U-rich upstream sequence elements on the pre-mRNA
	a core CPSF complex comprising CPSF1, WDR33, CPSF4 and FIP1L1 is sufficient for AAUAAA motif recognition, necessary for 3' polyadenylation, a key step in eukaryotic mRNA biogenesis

INTERACTION

DNA

RNA	binding to the AAUAA motif of the pre mRNA,upstream the cleavage site

small molecule

protein	CPSF1, binding the polyadenylation signal AAUAAA, and PABPN1, binding the growing poly(A) tail, cooperatively stimulate poly(A) polymerase such that a complete poly(A) tail is synthesized in one processive event, which terminates at a length of approximately 250 nucleotides
	CPSF1 interaction with intronic IL7R pre-mRNA interferes likely with spliceosome binding to the exon 6 5' splice site

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

MYP27

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function constitutional     --low   in diabetic retinopathy (DR) tissues and retinal vascular endothelial cells &65288;HRVECs&65289; under high-glucose (HG) conditions tumoral     --low   depletion of CPSF1 suppressed cell viability, impaired colony formation ability, induced cell cycle arrest at G0/G1 phase and promoted cell apoptosis in ovarian cancer cells

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed cancer reproductive ovary its depletion might serve as a potential therapeutic target for human ovarian cancer

ANIMAL & CELL MODELS