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FLASH GENE
Symbol CKAP2 contributors: mct - updated : 13-10-2014
HGNC name cytoskeleton associated protein 2
HGNC id 1990
DNA
TYPE virus associated
STRUCTURE 21.27 kb     9 Exon(s)
10 Kb 5' upstream gene genomic sequence study
regulatory sequence Promoter
cytosine-phosphate-guanine/HTF
text structure
  • upstream 115-bp region from the transcription start site was sufficient for minimal CKAP2 promoter activity
  • GC box was responsible for the cyclic activity of human CKAP2 promoter through the phosphorylation of Sp1, possibly by Cyclin A/Cdk complex
  • temporal change of GC box-binding affinity of Sp1 was responsible for the cyclic expression of human CKAP2
    • MAPPING cloned Y linked   status confirmed
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    9 splicing 3736 76 682 - Bae
  • using an alternate splice acceptor site at the beginning of the third coding exon
  • 9 exons
    • 9 splicing 3739 76 683 - Bae
  • using an alternate splice acceptor site at the beginning of the third coding exon resulting in a protein isoform that is longer by a single amino acid
    • 6 - 2073 - 494 - -
    EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveesophagus   moderately
     liver   highly
    Lymphoid/Immunelymph node   highly
     thymus    
    Reproductivemale systemtestis  highly
     male systemprostate  highly
    Urinarybladder   highly
     kidney   highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Blood / hematopoieticbone marrow  moderately
    Connectivebone  moderately
    Lymphoid    
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
    HOMOLOGY
    interspecies homolog to rattus Ckap2 (63.1 pc)
    homolog to murine Ckap2 (64.2 pc)
    Homologene
    FAMILY
    CATEGORY regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    text
  • microtubule-associated protein that localizes to spindle poles
  • expression of CKAP2 in the normal human tissues was limited to well-known cell proliferation zones
    • basic FUNCTION
  • involved in microtubule stabilization
  • involved in regulating aneuploidy, cell cycling, and cell death in a p53/TP53-dependent manner
  • plays a role in the assembly and maintenance of mitotic spindles, presumably by regulating microtubule dynamics, and its destruction during mitotic exit serves an important role in the completion of cytokinesis and in the maintenance of spindle bipolarity in the next mitosis (Hong 2007)
  • mitotic spindle-associated protein which is frequently up-regulated in various malignances
  • is essential for proper chromosome segregation and for maintaining genomic stability
  • involved in the assembly and maintenance of mitotic spindle and also plays an essential role in maintaining the fidelity of chromosome segregation during mitosis
  • plays crucial roles in proper chromosome segregation and maintaining genomic stability
  • microtubule-associated protein that localizes to spindle poles and aids in microtubule stabilization
  • is involved in the maintenance of microtubule nucleation sites, focusing microtubule minus ends to the spindle poles in early mitosis, and is implicated in maintaining genome stability
    • CELLULAR PROCESS cell cycle
    cell life, cell death/apoptosis
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • associating with alpha- and beta-tubulins
  • is a novel substrate of the Aurora B kinase
  • SP1 is a major regulator of periodic expression of CKAP2, and cell-cycle-dependent phosphorylation of SP1 mediated by CCNA2 can regulate the DNA-binding affinity of SP1
  • RPS6KA1 regulates anchorage-independent growth through transcriptional regulation of factors that modulate cell survival, including ING3, CKAP2, and PTK6
    • cell & other
    REGULATION
    Other cellular functions of CKAP2 might be regulated by timely phosphorylation and dephosphorylation during the course of mitosis
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --over  
    resulted in cell cycle arrest during mitosis due to a defect in centrosome separation and subsequent formation of a monopolar spindle (Hong 2007)
    tumoral     --over  
    in gastric cancers and large B-cell lymphoma
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS