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FLASH GENE
Symbol TJP1 contributors: mct/npt/pgu - updated : 15-06-2014
HGNC name tight junction protein 1 (zona occludens 1)
HGNC id 11827
Location 15q13.1      Physical location : 29.992.358 - 30.114.706
Synonym name
  • zonula occludens 1 protein
  • tight junction protein ZO-1
  • Synonym symbol(s) ZO1, ZO-1, MGC133289, DKFZp686M05161
    DNA
    TYPE functioning gene
    STRUCTURE 122.35 kb     28 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    Map cen - PWCR1 - TJP1 - qter
    Authors PMID: 8825647
    Physical map
    LOC390550 15 LOC390550 GABRG3 15q11.2-q12 gamma-aminobutyric acid (GABA) A receptor, gamma 3 OCA2 15q11.2-q12 oculocutaneous albinism II (pink-eye dilution homolog, mouse) LOC388091 15 LOC388091 HERC2 15q13 hect domain and RLD 2 RPL41P2 15q11-q13 ribosomal protein L41, pseudogene 2 LOC390551 15 similar to hect domain and RLD 2 LOC390552 15 similar to hypothetical protein FLJ36144 LOC388092 15 similar to hypothetical protein LOC342156 15q12 similar to ATP-binding cassette, sub-family B, member 10, mitochondrial precursor (ATP-binding cassette transporter 10) (ABC transporter 10 protein) (Mitochondrial ATP-binding cassette 2) (M-ABC2) LOC388093 15 similar to hypothetical protein MGC5244 LOC390553 15 hypothetical gene supported by NM_012089 LOC388094 15 similar to hypothetical protein LOC390554 15 similar to hypothetical protein FLJ36144 LOC388095 15 hypothetical gene supported by NM_004667 LOC342179 15q12 similar to KIAA0855 protein LOC390555 15 similar to Mothers against decapentaplegic homolog interacting protein (Madh-interacting protein) (Smad anchor for receptor activation) (Receptor activation anchor) (hSARA) (Novel serine protease) (NSP) LOC388096 15 LOC388096 LOC219392 15q12 similar to HP95 APBA2 15q14 amyloid beta (A4) precursor protein-binding, family A, member 2 (X11-like) NDNL2 15q11-q13 necdin-like 2 LOC390556 15 similar to tubulin, beta 5 LOC283697 15q13.1 hypothetical protein LOC283697 TJP1 15q13 tight junction protein 1 (zona occludens 1) LOC390557 15 similar to chromosome condensation protein G LOC283698 15q13.1 similar to synaptogyrin 2; cellugyrin LOC283717 15q13.1 similar to hypothetical protein FLJ35785 LOC390558 15 similar to Dynamin-1 (D100) (Dynamin, brain) (B-dynamin) LOC388097 15 similar to hyperpolarization activated cyclic nucleotide-gated potassium channel 2; brain cyclic nucleotide gated channel 2 CHRFAM7A 15q13-q14 CHRNA7 (cholinergic receptor, nicotinic, alpha polypeptide 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion LOC390559 15 similar to Dynamin-1 (D100) (Dynamin, brain) (B-dynamin) LOC388098 15 similar to hypothetical protein FLJ35785 LOC388099 15 similar to M-phase phosphoprotein 10 LOC342167 15q13.1 similar to hypothetical protein FLJ35785 LOC390560 15 similar to Dynamin-1 (D100) (Dynamin, brain) (B-dynamin) LOC388100 15 hypothetical gene supported by AF029838; NM_139320 LOC388101 15 hypothetical gene supported by NM_173613 LOC388102 15 hypothetical gene supported by NM_014783 LOC390561 15 similar to hypothetical protein FLJ35785 LOC388103 15 hypothetical gene supported by NM_004667 LOC388104 15 similar to chromosome 1 open reading frame 37 KIAA1018 15q12 similar to chromosome 1 open reading frame 37 FLJ20313 15q13.1 hypothetical protein FLJ20313 TRPM1 15q13-q14 transient receptor potential cation channel, subfamily M, member 1 LOC283710 15q13.1 hypothetical LOC283710 KLF13 15q13.3-q14 Kruppel-like factor 13 LOC283711 15q13.1 hypothetical protein LOC283711 LOC388105 15 hypothetical gene supported by BC009274; NM_020385 C15orf16 15q13.1 chromosome 15 open reading frame 16 LOC388106 15 hypothetical gene supported by BC035506; BC041580; NM_017779 CHRNA7 15q14 cholinergic receptor, nicotinic, alpha polypeptide 7
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    28 - 7165 - 1748 - 1993 8395056
    27 - 6925 - 1668 - 1993 8395056
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestivemouth   highly
     stomach   highly
    Nervousnervecranial nerve  highly
    Reproductivemale systemtestis   
    Respiratoryrespiratory tractlarynx  highly
    Urinarykidneynephronrenal capsuleglomerulus 
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Epithelialbarrier liningepidermisstratum corneum 
    Muscular    
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Muscularmyocyte
    Skin/Tegumenthair follicle cell
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N terminal (MAGUK-like),three Dlg,(DHR/GLGZ/PDZ) motifs, which are necessary for its function and playing a central role in assembling diverse protein complexes through their ability to recognize short peptide motifs on other proteins (PDZ2 domain binding to GJA1)
  • a SH3 domain
  • a unique proline-rich region cosedimenting with F-actin (ABR,
  • actin-binding region), a 220-AAs domain within the C-terminal half, mediating association with actin, at least in part
    HOMOLOGY
    interspecies homolog to yeast guanylate kinase
    homolog to Drosophila lethal discs large tumor suppressor protein
    Homologene
    FAMILY
  • MAGUK (membrane-associated guanylate kinase homolog) family
  • CATEGORY adhesion , chaperone/stress , structural protein
    SUBCELLULAR LOCALIZATION     plasma membrane,junction,tight
        intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus
    text
  • co-localized with JAM3 to adherens-like junctions
  • glomeruli predominantly to points where the slit diaphragm is inserted into the lateral cell membrane of the foot process
  • SYMPK co-localized with TJP1 at tight junctions
  • basic FUNCTION
  • F actin filament binding scaffold protein
  • structural component of tight junction and adherens junction
  • SHROOM2 and TJP1 form a tight-junction-associated scaffolding complex, possibly linked to myosin VIIa, that bridges the junctional membrane to the underlying cytoskeleton, thereby contributing to the stabilization of these junctions
  • colocalised in the salivary excretory ducts with CLDN16 and occludin suggesting a potential role in paracellular calcium and magnesium transport
  • central player in the control of gap junction (GJ) dynamics
  • regulates GJA1-mediated gap junctional communication in osteoblastic cells and alters the membrane localization of GJA1
  • essential for cytoskeletally-mediated barrier regulation
  • TJP1 and PLEKHA7 are paracingulin-interacting proteins that are involved in its recruitment to epithelial tight and adherens junctions, respectively
  • is a response protein to inner medullary tonicity and extracellular stressors can promote TJP1 protein expression, tyrosine phosphorylation and cytoskeleton association
  • TJP1 and TJP2 are required not only for TJ assembly but also for regulating the organization and functional activity of the apical cytoskeleton, particularly the perijunctional actomyosin ring
  • CPEB1-mediated TJP1 mRNA localization is essential for tight-junction assembly and mammary epithelial cell polarity
  • VCL and TJP1 anchor the actin cytoskeleton to the sarcolemma
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • ABCB1 and an integrated component of the occludin/zonula occludens 1 (TJP1) adhesion complex at the BTB, structurally interacted with PTK2, creating the OCLN/TJP1/PTK2/ABCB1 regulatory complex
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • binding directly to the C terminus of claudins
  • binding to occludin and alpha catenin
  • associating with F-actin (playing a role in several different steps of junction assembly)
  • bind to the C-terminus of OCLN
  • interaction with OCLN and PTPRJ specific (OCLN and TJP1 were dephosphorylated by PTPRJ but not the other phosphatases)
  • targets to gap junction edges independently of several known PDZ2-mediated interactions, including TJP1 homodimerization, heterodimerization with TJP2, and direct TJP1 binding to the C-terminal residues of GJA1) (H
  • by interacting with GJA1, plays a role in the down-regulation and decreased size of GJA1 gap junctions in congestive heart failure
  • interacting with KIRREL (tyrosine phosphorylation of KIRREL results in significantly increased KIRREL and TJP1 binding, suggesting a critical role for KIRREL tyrosine phosphorylation in reorganizing the KIRREL-TJP1 complex)
  • interacting with MYLK (MYLK activity is critical to TJP1, but not claudin-1, occludin, or actin) exchange at the tight junction)
  • interaction between TAZ and TJP1, TJP2, through TAZ C-terminal PDZ binding motif
  • TJP1 and the adherens junction protein PLEKHA7 as paracingulin-binding proteins
  • GJA8 requires an intact PDZ-binding motif and TJP1 for the formation of functional intercellular channels
  • interaction with UBN1 (N-terminal domains of the UBN1 and TJP1 proteins triggered a functional interaction inside the cell)
  • ARHGEF11 associates with tight junctions (TJs) by binding to TJP1, in polarized epithelial cells
  • KLF4 regulates blood-tumor barrier permeability via TJP1, OCLN and CLDN5
  • direct interaction between VCL and TJP1, illustrating how VCL plays a crucial role in stabilizing gap junctions and myocyte integrity
  • cell & other
    REGULATION
    Other upregulated, phosphorylated and linked to the actin cytoskeleton in response to hypertonic stress (
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --low  
    in patients with heart failure, with diminished expression levels of GJA1, suggesting that TJP1 plays an important role in gap junction formation and gap junction plaque stability
    constitutional     --other  
    present in apical tight junctions in normal epithelium but were dislocated to the basolateral position in patients with inactive Crohn disease (
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    networking between TAZ and TJP1 and TJP2 offers a promising target in the control of proliferation and apoptosis, epithelial-mesenchymal transition and migration capacity of cancer cells
    ANIMAL & CELL MODELS