motifs/domains
| twelve transmembrane segments (12TM) receptor |
|
intracytoplasmic N and C termini |
|
aminoacid permease domain |
|
a Py motif |
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coiled-coil dimerization domain |
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cytoplasmic tail containing at least two distinct major motifs, LLV motif controls ER export and cell surface expression levels, whereas another independent motif present in the region determines its polarized surface expression in epithelial cells  |
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a 150-AA stretch of SLC12A1 C-terminal tail is involved in the interaction with MAL |
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trihydrophobic motif in the distal C-terminus that was required for endoplasmic reticulum (ER) exit and surface expression of the co-transporter ; in the C-terminus, only motifs required for ER exit and surface expression of SLC12A1 are evolutionarily conserved in all members of the SLC12A family, a group of cation-chloride co-transporters |
| basic FUNCTION
| Na+ : K+ : 2Cl cotransporter 2, bumetanide-sensitive |
|
primary mediator of sodium and chloride reabsorption in the apical membrane thick ascending limb of the loop of Henle |
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having function in renal salt reabsorption |
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major route for sodium/chloride transport across the apical plasma membrane of the thick ascending limb (TAL)3 of the kidney |
|
Na-K-Cl cotransporter that mediates active reabsorption of sodium-chloride in the thick ascending limb of the loop of Henle in the kidney |
|
evidence of SLC12A1 expression in pancreatic beta-cells where it may play a role in insulin secretion |
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DNM2, CLTC and lipid rafts mediate SLC12A1 endocytosis and maintain steady-state apical surface SLC12A1 in native renal thick ascending limb (THAL) |
|
undergoes constitutive endocytosis in part via a clathrin-mediated pathway |
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endocytosis occurs at least in part via clathrin- and lipid raft-dependent pathways |
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couples the movement of Na(+), K(+), and Cl(-) ions across the plasma membrane of most cells and thus plays a central role in cellular homeostasis |
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furosemide-sensitive, and responsible for urine concentration and helps maintain systemic salt homeostasis |
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is one of the transport proteins with the highest overall reabsorptive capacity in the kidney |
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sodium reabsorption via SL C12A1 in the thick ascending limbs has a major role for medullary osmotic gradient and subsequent water reabsorption in the collecting ducts |
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osmoregulation requires brain expression of SLC12A1 |
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involved in insulin secretion and a single SLC12A2 allele may protect beta-cells from failure due to increased homeostatic expression of SLC12A1 |
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resorbs chloride with sodium and potassium ions at the apical membrane of epithelial cells in the kidney |
