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FLASH GENE

Symbol

MED25

contributors: mct/npt/pgu - updated : 28-09-2011

HGNC name	mediator complex subunit 25
HGNC id	28845

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Corresponding disease	CMT2B2 Charcot-Marie-Tooth disease, axonal, type 2B2
Location	19q13.33      Physical location : 50.321.535 - 50.340.237
Synonym name	ARC/mediator transcriptional coactivator subunit
	prostate-derived protein 78 kDa
	activator interaction domain 1 protein (ACID)
	mediator of RNA polymerase II transcription, subunit 25 homolog (yeast)
Synonym symbol(s)	P78, ACID1, TCBAP0758, DKFZp434K0512, MGC70671, ARC2, ARC92, DKFZp434K0512, PTOV2

DNA

TYPE	functioning gene
STRUCTURE	18.69 kb     18 Exon(s)

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_030973 18 - 2335 NP_112235 78 747 - 2002 12163014

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart       highly Lymphoid/Immune spleen       highly Nervous brain       highly Reproductive female system uterus cervix       female system placenta     highly Urinary kidney       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Muscular striatum skeletal   highly Nervous peripherous

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic leukocyte

cell lineage
cell lines	prostate cancer(much lower level in metastatic cancer compared to that in primary prostate cancer)
fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	two conserved structural domains with limited similarity to domain D of synapsin I
	an activator interaction domain (ACID), which forms a seven-stranded beta-barrel framed by three helices, and the VP16 subdomains H1 and H2 bind to opposite faces of ACID and cooperate during promoter-dependent activated transcription
	a MED25 domain that serves as a docking site on Mediator for the ATF6 transcription activation domain

HOMOLOGY

interspecies

homolog to murine Med25 (93.0pc)

Homologene

FAMILY

mediator complex subunit 25 family

CATEGORY

chaperone/stress , regulatory

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleoplasm

basic FUNCTION	associating with the activation domains of multiple cellular and viral transcriptional activators, including the herpes simplex viral activator VP16, mammalian cells,
	required for RARA/RXRA-mediated transcription
	serving as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors
	potential role of MED25 in gene dosage sensitive peripheral neuropathy pathogenesis
	MED25 and PTOV1 reciprocally regulate RAR transcriptional activity through competitive bindings to CREBBP and opposite regulation of CREBBP recruitment to the RA(Retinoic acid)-responsive gene promoter
	MED25 and PTOV1 differentially modulate RA sensitivity in cancer cells depending on their expression levels
	role for MED25 and the Mediator complex in the regulation of xenobiotic metabolism and lipid homeostasis

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

component of the mediator complex

INTERACTION

DNA

RNA

small molecule

protein	interacting with CREBP, ESR1, GR, RARA, RXRA and THRB in a ligand-dependent manner
	required for the association of HNF4A with Mediator, its several cofactors, and RNA Pol II
	critical target of the potent herpes simplex 1 viral transcriptional activator VP16
	regulatory interaction between SOX9, WWP2 and MED25 defines the SOX9 transcriptional mechanisms of chondrogenesis in the forming palate
	MED1 and MED25, regulate a restricted cohort of genes that are predominantly not controlled by GATA1
	ATF6-MED25 interaction contributes to Mediator recruitment to genes

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

CMT2B2

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS