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FLASH GENE

Symbol

CEP55

contributors: mct/npt/pgu - updated : 18-09-2017

HGNC name	centrosomal protein 55kDa
HGNC id	1161

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	10q23.33      Physical location : 95.256.368 - 95.288.847
Synonym name	chromosome 10 open reading frame 3
	up-regulated in colon cancer 6
	cancer/testis antigen 111
Synonym symbol(s)	FLJ10540, C10orf3, URCC6, CT111

DNA

TYPE	functioning gene
STRUCTURE	32.48 kb     9 Exon(s)

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_018131 9 - 2656 NP_060601 54 464 - 2006 16406728 NM_001127182 9 - 2534 NP_001120654 - 464 - 2006 16406728

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive mouth tongue     highly Reproductive female system uterus cervix   highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective bone

cell lineage
cell lines	various cancer
fluid/secretion

at STAGE

physiological period

fetal

Text	heart and spleen

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	three central coiled-coil domains responsible for protein-protein interactions
	a domain known as AAA (ATPases associated with a variety of cellular activities)

conjugated

PhosphoP

HOMOLOGY

Homologene

FAMILY

CATEGORY

structural protein

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
	intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
text	localizes to the mitotic spindle during prometaphase and metaphase and to the spindle midzone and the midbody during anaphase and cytokinesis
	localized to the centrosome in interphase cells

basic FUNCTION	playing a role in cytokinesis as its depletion by siRNA results in failure of this process
	contributing to spindle pole organization and playing a role in cell cycle progression as well as completing cytokinesis
	involved in in centrosome-dependent cellular functions, such as centrosome duplication and/or cell cycle progression, or in the regulation of cytokinesis
	regulator required for the completion of cytokinesis
	microtubule-associated protein that efficiently bundles microtubules
	plays an essential role in the G2/M phase of the cell cycle and display dynamic cellular distribution patterns during cell-cycle progression
	mitotic phosphoprotein that plays an important role in cytokinesis, the final stage of cell division during which physical separation of the two daughter cells is accomplished
	regulator required for the completion of cell abscission

CELLULAR PROCESS	cell cycle, progression

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

ESCRT-III (CHMP) proteins pathhway

a component	capable of homodimerization
	complexing with PI3K

INTERACTION

DNA

RNA

small molecule

protein	interacting with EGFP in all stages of mitosis
	interacting with TSG101 (downregulated TSG101 in a post-transcriptional manner)
	forms a complex with PI3K and can activate PI3K activity
	associates with the KIF23-RACGAP1 centralspindlin complex
	linking RACGAP1 through a cellular mechanism which, in turn, controls the midbody structure and membrane fusion at the terminal stage of cytokinesis
	PLK1 activity negatively regulates CEP55 to ensure orderly abscission factor recruitment and ensures that this occurs only once cell contraction has completed
	TP53-PLK1-CEP55 axis in which TP53 negatively regulates expression of CEP55, through PLK1 which, in turn, is a positive regulator of CEP55 protein stability
	associates with and is phosphorylated by PLK1 during mitosis (tight regulation of CEP55 protein levels by TP53 is mediated by PLK1 phosphorylation)
	BRCA2 localization to the midbody by FLNA regulates CEP55 signaling and completion of cytokinesis
	TEX14 competitively binds to CEP55-EABR to prevent the recruitment of PDCD6IP, which is a component of the ESCRT machinery with the AxGPPx3Y motif
	DDX21 induces CEP55 expression, and MYCN-amplified neuroblastoma cell proliferation and tumorigenesis

cell & other

directly interacting with centrosome components rather than with microtubules

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in colorectal cancers (CRC) as well as in tumors arising in the stomach, lung, pancreas, and breast tumoral     --over   in hepatocellular carcinoma tumoral     --over   overexpressed inoral squamous cell carcinomas, thus stimulating cell migration and invasion through increased FOXM1 and MMP2 expression

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed cancer brain glioma/neuroblstoma DDX21 and CEP55 are valid therapeutic targets for the treatment of MYCN-amplified neuroblastoma cancer digestive liver . potential new therapeutic target for hepatocellular carcinoma treatment and may contribute to the development of new therapeutic strategies that are able to block the PI3K/AKT pathway in cancer cells cancer head and neck   novel therapeutic target for oral squamous cell carcinomas (OCSCC) because suppression of CEP55 could cause significant inhibition on cell migratory and invasive ability in oral cancer cells

ANIMAL & CELL MODELS