Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

PPP1R8

contributors: mct/npt - updated : 11-02-2009

HGNC name	protein phosphatase 1, regulatory (inhibitor) subunit 8
HGNC id	9296

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	1p35.3      Physical location : 28.157.292 - 28.178.183
Synonym name	activator of RNA decay 1
	nuclear inhibitor of protein Ser/Thr phosphatase-1
	RNase E
	nuclear subunit of PP-1
Synonym symbol(s)	ARD1, NIPP1, PRO2047, NIPP-1
EC.number	3.1.4.-

DNA

TYPE	functioning gene
STRUCTURE	20.90 kb     7 Exon(s)

Genomic sequence alignment details
10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed isoform alpha 7 splicing 2336 38 351 - Van Eynde, Nuytten (2008) seven exons isoform beta 7 splicing 2691 - 209 brain, heart, kidney Van Eynde, Nuytten (2008) isoform gamma/and 1 6 splicing 2590 13.3 127 B-cells, t-lymphocytes and 293 embryonic kidney cells Wang, Nuytten (2008) implicated in mRNA splicing site-specific single-strand endonuclease mostly cytoplasmic

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart         Endocrine neuroendocrine pituitary     highly Lymphoid/Immune thymus       highly Nervous brain         Urinary kidney

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective adipose     highly Muscular striatum cardiac     Nervous central

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES		acid ,basic
STRUCTURE

motifs/domains	a basic N and C-terminal
	an acidic central domains
	a FHA domain
	a C-terminal fragment displaying an RNase E like endoribonuclease activity

mono polymer

complex

HOMOLOGY

interspecies

homolog to C.elegans B0511.7

Homologene

FAMILY

CATEGORY

enzyme , regulatory , RNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm
	intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles

basic FUNCTION	type 1 serine/threonine specific protein phosphatase inhibitor, involved in a late step of spliceosome assembly
	playing a role as a DNA-targeting protein for EED and associated chromatin-modifying enzymes
	nuclear scaffold protein implicated in both transcription and RNA processing
	playing an indispensable role for early embryonic development and cell proliferation

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

RNA catabolism (RNA turnover)

a component

inhibitor subunit of the major nuclear protein phosphatase-1 (pp.1)

INTERACTION

DNA

RNA

small molecule	metal binding, cofactor,
	Mg2+

protein	interacting with PP1
	interacting with the splicing factors SAP155 and CDC5L
	NIPP1-EED interaction potentiated by the binding of (d)G-rich nucleic acids to the central domain of PPP1R8
	promotes SF3B1 dephosphorylation (specifically recognizes hyperphosphorylated SF3B1 thorough its Forkhead-associated domain and dissociates from SF3B1 after dephosphorylation by associated protein phosphatase-1)
	associates with a subset of PcG (Polycomb group) targets in a PP1-dependent manner and thereby contributes to the recruitment of the PRC2 complex

cell & other

REGULATION

inhibited by

downregulated by phosphorylation

Other

tightly regulated by phosphorylation

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in lung, colorectal, gastric, ovarian carcinoma

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS