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FLASH GENE
Symbol UBA5 contributors: mct - updated : 13-10-201
HGNC name ubiquitin-like modifier activating enzyme 5
HGNC id 23230
EXPRESSION
Type
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestiveintestinelarge intestinecolon  
Reproductivemale systemtestis   
Respiratorylung    
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • an adenylation domain
  • AAs 364-404 were demonstrated to be necessary for the transthiolation of UFM1 to UFC1
  • UBA5 AA 381-404 was identified to be the minimal region for UFC1 recognition
  • a C-terminal region
    • HOMOLOGY
    interspecies homolog to murine Ube1dc1
    homolog to rattus LOC300968
    Homologene
    FAMILY
  • ubiquitin-activating E1 family
  • UBA5 subfamily
    • CATEGORY enzyme
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus
    basic FUNCTION
  • may have an oxidoreductase activity and be involved in L-serine biosynthesis
  • activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (UFM1), by forming a high-energy thioester bond
  • essential role, as a specific activating enzyme for the ubiquitin-like modifier, UFM1, in erythroid development
  • dispensable for the production of erythropoietin, but its genetic loss led to impaired development of megakaryocyte and erythroid progenitors from common myeloid progenitors
  • smallest ubiquitin-like molecule-activating enzyme
  • E1-activating enzyme of ubiquitin-fold modifier 1 (UFM1), a recently identified ubiquitin-like protein
  • role of UBA5 dimerization in UFM1 activation
    • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism aminoacid
    signaling
    UFM1-UBA5-UFC1 ubiquination pathway
    a component
  • fusion protein between UBA5 381-404 and UFC1 exists as a homodimer in solution
    • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • activate another ubiquitin-like protein, SUMO2, besides UFM1 (greatly activated SUMO2 in the nucleus or transferred activated-SUMO2 to nucleus after it conjugated SUMO2 in the cytoplasm)
  • UFM1 covalently attach with cellular protein(s) via a specific E1-activating enzyme (UBA5) and an E2-conjugating enzyme (UFC1)
  • UFM1 is activated by the E1 enzyme UBA5, and this pathway is proposed to play an important role in the endoplasmic reticulum (ER) stress response
  • Ufmylation is initiated by UBA5, which activates and transfers UFM1 to ubiquitin-fold modifier-conjugating enzyme 1 (UFC1)
  • UBA5 binds to UFM1 via a trans-binding mechanism in which UFM1 interacts with distinct sites in both subunits of the UBA5 dimer
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s) SCAR24 , EIEE44
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • mice lacking Uba5 exhibited severe anaemia, followed by death in utero
  • Uba5 silencing in zebrafish decreased motility while inducing abnormal movements suggestive of seizures