Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

CENPF

contributors: mct - updated : 04-01-2016

HGNC name	centromere protein F, 350/400ka (mitosin)
HGNC id	1857

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_016343 20 - 10316 350 3114 - 2007 17600710

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart       highly Digestive intestine small intestine     highly Lymphoid/Immune lymph node       highly   thymus       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective bone

cell lineage

cell lines

fluid/secretion

at STAGE

cell cycle

cell cycle, interphase, G2

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	two long coil domains that flank a central flexible core
	multiple leucine zipper motifs potentially for protein interactions
	a putative P-loop nucleotide binding site within the globular C terminus
	C-terminal portion between residues 2488 and 3113 bound to ATF4 through two distinct domains, one of which was a leucine zipper motif

mono polymer

homomer , heteromer , dimer

HOMOLOGY

interspecies

homolog to murine Cenpf

Homologene

FAMILY

CATEGORY

structural protein

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytosolic
	intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
	intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
	intracellular,nucleus,chromatin/chromosome,centromere
	intracellular,nucleus,chromatin/chromosome,kinetochore
text	localization of CENPF at the centrosome
	in M phase it localizes to the kinetochore, the major microtubule-binding structure on chromosomes essential for chromosome segregation
	nuclear matrix protein in interphase cells

basic FUNCTION	may be playing a role in chromosome segregation during mitotis
	multifunctional protein involved in mitotic control, microtubule dynamics, transcriptional regulation, and muscle cell differentiation
	farnesylation plays a global role in CENPF function
	role of CENP-F in regulation of epigenetic modification on histone H3
	role in organization of interphase chromatin through association and possibly regulation of PRKDC
	epistatic roles for CENPF and RAB5A in the pathway that orchestrates chromosome congression
	RHOT1 and CENPF promote anterograde mitochondrial movement and proper mitochondrial distribution in daughter cells

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	constituent of the outer kinetochore plate
	component of the nuclear matrix during the G2 phase of interphase, in late G2 associates with the kinetochore and maintains this association through early anaphase
	NUP107-160 complex interacts with CENPF, but CENPF only moderately contributes to its targeting to kinetochores
	component of the nuclear matrix required for chromosome congression that, at mitotic entry, localizes to the nuclear envelope and assembles on kinetochores, contributing to the establishment of kinetochore microtubule interactions

INTERACTION

DNA

RNA

small molecule

protein	RB, CENPE, BUB1B
	may be a negative regulator of ATF4 in interphase through direct interaction
	PARP1 interaction with CENPB, CENPE, and CENPF during mitosis and apoptosis
	interacting with E2F5 (CENPF translocation to the nucleus is an important first step during entry into quiescence)
	HOOK2 is a binding partner of CENPF
	major function of CENPF, is to link the NDEL1/NDE1/PAFAH1B1/Dynein pathway to kinetochores (NDEL1 and NDE1 play distinct roles to ensure chromosome alignment and segregation)
	critical for myocyte differentiation through the interaction with RB, and binds to ATF4 and negatively regulates the transcriptional activity of ATF4
	associated with the DNA-dependent protein kinase (PRKDC), a trimeric protein complex critical for genome homeostasis
	CENPF is recruited to mitochondria by RHOT1 at the time of cytokinesis and associates with microtubule growing tips
	CHAMP1 regulates localization of CENPE and CENPF to kinetochores
	SYCP2 N-reminal region interacts with the centromere during meiosis I, with CENPJ and CENPF
	NUP133 interacts with CENPF both at nuclear pores in prophase and at kinetochores in mitosis

cell & other

REGULATION

Other	post-anaphase, KEN-box-dependent degradation of CENPF requires it to be farnesylated, a post-translational modification usually linked to membrane association

ASSOCIATED DISORDERS

corresponding disease(s)

ICS32

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral   amplification     and overexpressed in esophageal squamous cell carcinomas

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS