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FLASH GENE
Symbol CENPF contributors: mct - updated : 04-01-2016
HGNC name centromere protein F, 350/400ka (mitosin)
HGNC id 1857
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
20 - 10316 350 3114 - 2007 17600710
EXPRESSION
Type widely
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Cardiovascularheart   highly
Digestiveintestinesmall intestine  highly
Lymphoid/Immunelymph node   highly
 thymus   highly
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Connectivebone   
cell lineage
cell lines
fluid/secretion
at STAGE
cell cycle     cell cycle, interphase, G2
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • two long coil domains that flank a central flexible core
  • multiple leucine zipper motifs potentially for protein interactions
  • a putative P-loop nucleotide binding site within the globular C terminus
  • C-terminal portion between residues 2488 and 3113 bound to ATF4 through two distinct domains, one of which was a leucine zipper motif
  • mono polymer homomer , heteromer , dimer
    HOMOLOGY
    interspecies homolog to murine Cenpf
    Homologene
    FAMILY
    CATEGORY structural protein
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,centromere
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • localization of CENPF at the centrosome
  • in M phase it localizes to the kinetochore, the major microtubule-binding structure on chromosomes essential for chromosome segregation
  • nuclear matrix protein in interphase cells
  • basic FUNCTION
  • may be playing a role in chromosome segregation during mitotis
  • multifunctional protein involved in mitotic control, microtubule dynamics, transcriptional regulation, and muscle cell differentiation
  • farnesylation plays a global role in CENPF function
  • role of CENP-F in regulation of epigenetic modification on histone H3
  • role in organization of interphase chromatin through association and possibly regulation of PRKDC
  • epistatic roles for CENPF and RAB5A in the pathway that orchestrates chromosome congression
  • RHOT1 and CENPF promote anterograde mitochondrial movement and proper mitochondrial distribution in daughter cells
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • constituent of the outer kinetochore plate
  • component of the nuclear matrix during the G2 phase of interphase, in late G2 associates with the kinetochore and maintains this association through early anaphase
  • NUP107-160 complex interacts with CENPF, but CENPF only moderately contributes to its targeting to kinetochores
  • component of the nuclear matrix required for chromosome congression that, at mitotic entry, localizes to the nuclear envelope and assembles on kinetochores, contributing to the establishment of kinetochore microtubule interactions
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • RB, CENPE, BUB1B
  • may be a negative regulator of ATF4 in interphase through direct interaction
  • PARP1 interaction with CENPB, CENPE, and CENPF during mitosis and apoptosis
  • interacting with E2F5 (CENPF translocation to the nucleus is an important first step during entry into quiescence)
  • HOOK2 is a binding partner of CENPF
  • major function of CENPF, is to link the NDEL1/NDE1/PAFAH1B1/Dynein pathway to kinetochores (NDEL1 and NDE1 play distinct roles to ensure chromosome alignment and segregation)
  • critical for myocyte differentiation through the interaction with RB, and binds to ATF4 and negatively regulates the transcriptional activity of ATF4
  • associated with the DNA-dependent protein kinase (PRKDC), a trimeric protein complex critical for genome homeostasis
  • CENPF is recruited to mitochondria by RHOT1 at the time of cytokinesis and associates with microtubule growing tips
  • CHAMP1 regulates localization of CENPE and CENPF to kinetochores
  • NUP133 interacts with CENPF both at nuclear pores in prophase and at kinetochores in mitosis
  • cell & other
    REGULATION
    Other post-anaphase, KEN-box-dependent degradation of CENPF requires it to be farnesylated, a post-translational modification usually linked to membrane association
    ASSOCIATED DISORDERS
    corresponding disease(s) ICS32
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral   amplification    
    and overexpressed in esophageal squamous cell carcinomas
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS