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FLASH GENE
Symbol ZW10 contributors: mct/npt - updated : 17-12-2014
HGNC name ZW10, kinetochore associated, homolog (Drosophila)
HGNC id 13194
Location 11q23.2      Physical location : 113.603.910 - 113.644.425
Synonym name
  • centromere/kinetochore protein zw10
  • zeste white 10 homolog
  • ZW10 homolog, centromere/kinetochore protein (Drosophila)
  • Synonym symbol(s) HZW10, KNTC1AP, MGC149821, DSL1
    DNA
    TYPE functioning gene
    STRUCTURE 40.52 kb     16 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    Map cen - D11S1347 - DLAT - CRYAB - D11S938 - D11S1327 - APOC3 APOC3 - ZW10 - D11S1340 - IL10RA - D11S939 - qter
    Authors Gene Map (98)
    Physical map
    IL18 11q22.2-q22.3 interleukin 18 (interferon-gamma-inducing factor) TEX12 11q22 testis expressed sequence 12 BCDO2 11q22.3-q23.1 beta-carotene dioxygenase 2 MRPS36P4 11q23.2 mitochondrial ribosomal protein S36 pseudogene PTS 11q22.3-q23.3 6-pyruvoyltetrahydropterin synthase PLET1 11q23.2 placenta expressed transcript 1 LOC387809 11 similar to heat shock 70kD protein binding protein; progesterone receptor-associated p48 protein; putative tumor suppressor ST13; Hsp70-interacting protein LOC387810 11 LOC387810 NCAM1 11q23.1 neural cell adhesion molecule 1 TTC12 11q23.2 tetratricopeptide repeat domain 12 ANKK1 11q23.2 ankyrin repeat and kinase domain containing 1 DRD2 11q23.1 dopamine receptor D2 TMPRSS5 11q23 transmembrane protease, serine 5 (spinesin) ZW10 11q22-q23.3 ZW10 homolog, centromere/kinetochore protein (Drosophila) LOC255244 11q23.3 similar to Claudin-22 LOC390251 11 hypothetical gene supported by AK093353 LOC143920 11q23.3 similar to activating transcription factor 4; cAMP response element-binding protein 2 USP28 11q23 ubiquitin specific protease 28 HTR3B 11q23.1-q23.2 5-hydroxytryptamine (serotonin) receptor 3B HTR3A 11q23.1-q23.2 5-hydroxytryptamine (serotonin) receptor 3A ZNF145 11q23.1 zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia) NNMT 11q23.1 nicotinamide N-methyltransferase FLJ20010 11q14.2-q14.3 hypothetical protein FLJ20010 RBM7 11q23.1-q23.2 RNA binding motif protein 7 DKFZP566E144 11q23.1-q23.2 small fragment nuclease MGC34290 11q23.3 hypothetical protein MGC34290 C11orf33 11q23.3 chromosome 11 open reading frame 33 LOC390252 11 similar to putative protein family member (XC187) LOC390253 11 similar to putative protein family member (XC187) FLJ25224 11q23.3 hypothetical protein FLJ25224 IGSF4 11q23.2 immunoglobulin superfamily, member 4
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    16 - 2900 - 779 - 2008 18782227
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivemouthtongue  highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectivebone  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    cell cycle     cell cycle, M
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • kinetochore localization domain is the ZWINT interaction domain (Famulski 2008)
  • C-terminal region interacts with dynamitin (DCTN2), and part of this region has been used as an inhibitor of ZW10 function (Inoue 2008)
  • HOMOLOGY
    interspecies homolog to Drosophila zeste-white 10
    Homologene
    FAMILY
  • ZW10 family
  • CATEGORY regulatory , DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,centromere
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • moving from the centromere/kinetochore at prometaphase to kinetochore microtubules at metaphase and then back to the centromere/kinetochore at anaphase
  • kinetochore residency at prometaphase kinetochores is dependent on its interaction with ZWINT, and is essential for mitotic checkpoint arres (Famulski 2008)
  • located in the endoplasmic reticulum as well as in the cytosol during interphase (Hirose 2004)
  • ZWINT localizes to the kinetochore in prophase, before ZW10 localization, and remains at the kinetochore until anaphase, after ZW10 has dissociated
  • basic FUNCTION
  • centromere/kinetochore protein, recruiting dynein and dynactin to the centromere
  • functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor
  • ZW10 and KNTC1 play an essential role in mitotic checkpoint
  • ZW10 and KNTC1 proteins are part of a complex necessary for accurate chromosome segregation
  • involved in membrane trafficking between the endoplasmic reticulum and Golgi.and playing a role during interphase (Hirose 2007)
  • implicated in anchoring of MAD1L1/MAD1L2 to the kinetochore and appears to also move off the kinetochore with MAD2L1 (Sivaram 2009)
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis
  • member of the RZZ complex (Rod–Zw10–Zwilch) (Sivaram 2009)
  • forms a subcomplex with RINT1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking (Hirose 2004)
  • RZZ (KNTC1/ZW10/ZWILCH) complex is required for the recruitment of cytoplasmic dynein to kinetochores
  • part of NRZ complex, which comprises KNTC1, RINT1, and ZW10, is also involved in Golgi-to-ER retrograde transport, but each component of the complex has diverse cellular functions including endosome-to-Golgi transport, cytokinesis, cell cycle checkpoint, autophagy, and mRNA decay
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with ZWINT (specifies the kinetochore association of ZW10 by interacting with its N-terminal domain)
  • weak interaction between the ZW10/KNTC1/ZWILCH complex and the kinesin-like kinetochore component CENPE
  • ZWINT is critical for recruiting ZW10 to unattached kinetochores
  • AURKB activity is required for the accumulation of tension-sensitive mitotic-checkpoint components, such as ZW10 and KNTC1, in order to maintain mitotic-checkpoint arrest
  • interacts with dynamitin, a subunit of the dynein accessory complex dynactin, and functions in termination of the spindle checkpoint during mitosis and in membrane transport between the endoplasmic reticulum (ER) and Golgi apparatus during interphase (Inoue 2008)
  • ZW10 kinetochore residency at prometaphase kinetochores is dependent on its interaction with ZWINT, and is essential for mitotic checkpoint arrest
  • NBAS links between USE1 and ZW10-RINT1 and is involved in Golgi-to-ER transport
  • CCDC99 is a cell cycle-regulated mitotic phosphoprotein that interacts with the KNTC1/ZW10/ZWILCH (RZZ) complex
  • PIAS4 interacts with the KNTC1/ZW10 complex
  • ZWINT, a kinetochore protein interact with the kinetochore checkpoint protein ZW10
  • RINT1 mediates the association of ZW10 (mammalian Dsl1) with endoplasmic reticulum-localized SNARE proteins
  • cell & other
    REGULATION
    Other regulated by RAB6 (both ZW10/RINT1 and conserved oligomeric Golgi complex-dependent Golgi trafficking and homeostasis) (Sun 2007)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS