8722;KTS) results in an increase in trascriptional activation mediated by WT1, implying that ZNF224 acts as a co-regulator of WT1
-
splicing
-
-
622
. in fetal brain, kidney, muscle
. distributed throughout the cell and is present also in nucleoli and cytosol
2007
17900823
spans the region between amino acids 86 and 707 of ZNF224
having 19 zinc-finger domains of ZNF224, lacks the KRAB A domain and possesses a KRNB located at its amino terminus, which may function as a transregulatory domain
potential role in RNA processing together with WT1
involvement in a specific molecular pathway, RNA maturation and processing
interacts and co-localizes with both WT1 isoforms, with a preference for the (+KTS) isoform, suggesting a role for this interaction in RNA maturation and post-transcriptional control
ZNF224 and ZNF255 have a distinct pattern of distribution within the cell and display a specific pattern of interaction with different molecular partners.
EXPRESSION
Type
ubiquitous
expressed in
(based on citations)
organ(s)
System
Organ level 1
Organ level 2
Organ level 3
Organ level 4
Level
Pubmed
Species
Stage
Rna symbol
Hearing/Equilibrium
ear
inner
cochlea
highly
Lymphoid/Immune
thymus
highly
tissue
System
Tissue
Tissue level 1
Tissue level 2
Level
Pubmed
Species
Stage
Rna symbol
Blood / Hematopoietic
bone marrow
Muscular
striatum
highly
cell lineage
cell lines
Hela cells
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
a Krüppel-associated box (KRAB) domain at the NH2 terminus, including a canonical box A and a degenerated box b
one KRAB domain
19 Cys2-His2 zinc-finger domains at the C-terminus
represses aldolase A gene transcription by interacting with the TRIM28 co-repressor protein
repressing the transcription of the SLC25A1 gene and so may be responsible for the low expression of SLC25A1 in fetal tissues in which fatty acid synthesis is low
with its shorter isoform, ZNF255, differentially repress aldolase gene transcription and exhibit a distinctive subcellular localization
may act as a transcriptional co-regulator of WT1(&
8722;KTS)
multifunctional protein and the alternative splicing, sub-cellular compartmentalization and isoform-specific interactions may modulate its activity
ZNF224 can be a mediator of DNA damage-induced apoptosis in leukemia cells
CELLULAR PROCESS
nucleotide, transcription, regulation
PHYSIOLOGICAL PROCESS
PATHWAY
metabolism
signaling
a component
INTERACTION
DNA
binding
RNA
small molecule
metal binding,
Zn2+
protein
direct interaction between the arginine methyltransferase PRMT5 and ZNF224 is required for repression of aldolase A gene transcription
WT1-associating protein
binding to the cis-negative regulatory element of aldolase A (AldA-NRE), and transcriptional repressor of the human aldolase A gene
PRMT5 was demonstrated to play a crucial role in the transcriptional ZNF224 repressor complex
acts in fine tuning of WT1-dependent control of gene expression, acting as a co-activator of WT1 in the regulation of proapoptotic genes and suppressing WT1 mediated trans-activation of antiapoptotitc genes
