SUBCELLULAR LOCALIZATION
| intracellular
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| intracellular,cytoplasm,organelle,endosome
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| intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
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text
| localizes to endosomal subdomains (Jia 2010) |
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TUBG1 and Arp2/3 colocalize at the centrosome, where WASH1 localizes |
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colocalizes on endosomal subdomains with the cargo-selective complex (CSC) of the retromer, where it regulates retrograde sorting from endosomes in an actin-dependent manner |
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basic FUNCTION
| generates an actin network on a restricted domain of sorting and recycling endosomes (Derivery 2009) |
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controls the fission of endosomes through an interplay between the forces generated by microtubule motors and actin polymerization (Derivery 2009) |
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Arp2/3 activator, localized with activated receptors, but not essential for endocytosis (Gomez 2009) |
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regulates endocytic vesicle scission in an Arp2/3-dependent manner (Jia 2010) |
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associated with vesicular F-actin, and regulates retromer-mediated vesicle trafficking through an interaction with the protein FAM21C (Jia 2010) |
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functions in cells within a 500 kDa core complex containing Strumpellin, FAM21C, KIAA1033 (SWIP), and CCDC53 (Jia 2010) |
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actin polymerization by WASH1 influences the shape and maturation of endosomes, suggesting a previously unrecognized role for WASH1 and the Arp2/3 complex in the degradative steps of endocytic trafficking |
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causes actin to polymerize on vesicles involved in retrograde traffic and exocytosis. |
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important regulator of vesicle trafficking, and is able to directly regulate endosomal sorting and maturation |
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is required for the maturation of lysosomes to a stage at which hydrolases can be retrieved and reused |
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WASH1 and exocyst are required for matrix degradation by an exocytic mechanism that involves tubular connections between MMP14–positive late endosomes and the plasma membrane in contact with the matrix |
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WASH1 recruitment of RNF2 for AMBRA1 degradation leading to downregulation of autophagy |