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FLASH GENE
Symbol WASH1 contributors: mct - updated : 21-11-2014
HGNC name WAS protein family homolog 1
HGNC id 24361
Location 9p24.3      Physical location : 14.510 - 29.739
Synonym name
  • CXYorf1-like protein on chromosome 9
  • family with sequence similarity 39, member E
  • Wiskott–Aldrich syndrome protein and SCAR homologue
  • FLJ00075 protein
  • protein FAM39E
  • Synonym symbol(s) DKFZp434K1323, FAM39E, FLJ00038, FLJ75027, FLJ99609, MGC190539, MGC193256, WASH
    DNA
    TYPE functioning gene
    STRUCTURE 15.23 kb     11 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    11 - 1837 51 465 - 2009 19922874
    EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a conserved C-terminal VCA (Verprolin homologous, central hydrophobic, and acidic) region for stimulating Arp2/3 complex (Jia 2010)
  • HOMOLOGY
    Homologene
    FAMILY
  • Wiskott-Aldrich syndrome protein (WASP) family
  • FAM39 family
  • CATEGORY regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,endosome
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    text
  • localizes to endosomal subdomains (Jia 2010)
  • TUBG1 and Arp2/3 colocalize at the centrosome, where WASH1 localizes
  • colocalizes on endosomal subdomains with the cargo-selective complex (CSC) of the retromer, where it regulates retrograde sorting from endosomes in an actin-dependent manner
  • basic FUNCTION
  • generates an actin network on a restricted domain of sorting and recycling endosomes (Derivery 2009)
  • controls the fission of endosomes through an interplay between the forces generated by microtubule motors and actin polymerization (Derivery 2009)
  • Arp2/3 activator, localized with activated receptors, but not essential for endocytosis (Gomez 2009)
  • regulates endocytic vesicle scission in an Arp2/3-dependent manner (Jia 2010)
  • associated with vesicular F-actin, and regulates retromer-mediated vesicle trafficking through an interaction with the protein FAM21C (Jia 2010)
  • functions in cells within a 500 kDa core complex containing Strumpellin, FAM21C, KIAA1033 (SWIP), and CCDC53 (Jia 2010)
  • actin polymerization by WASH1 influences the shape and maturation of endosomes, suggesting a previously unrecognized role for WASH1 and the Arp2/3 complex in the degradative steps of endocytic trafficking
  • causes actin to polymerize on vesicles involved in retrograde traffic and exocytosis.
  • important regulator of vesicle trafficking, and is able to directly regulate endosomal sorting and maturation
  • is required for the maturation of lysosomes to a stage at which hydrolases can be retrieved and reused
  • WASH1 and exocyst are required for matrix degradation by an exocytic mechanism that involves tubular connections between MMP14–positive late endosomes and the plasma membrane in contact with the matrix
  • WASH1 recruitment of RNF2 for AMBRA1 degradation leading to downregulation of autophagy
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacts with dynamin (Derivery 2009)
  • FAM21A, FAM1B, FAM21C, FAM21D and WASH1 act at different steps of a cyclical pathway in which FAM21 mediates recycling of the complex back to acidic lysosomes
  • can suppress BECN1 ubiquitination to inactivate PIK3C3 activity leading to suppression of autophagy
  • function in endosomal sorting via Arp2/3 activation
  • WASH1 is a new interactor of BECN1 and present in the BECN1-PIK3C3 complex with AMBRA1
  • RNF2 potentiates AMBRA1 degradation with the help of WASH1
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS