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FLASH GENE
Symbol VAMP8 contributors: mct/pgu - updated : 20-06-2013
HGNC name vesicle-associated membrane protein 8 (endobrevin)
HGNC id 12647
Location 2p11.2      Physical location : 85.804.613 - 85.809.155
Synonym name endobrevin
Synonym symbol(s) EDB, VAMP-8
DNA
TYPE functioning gene
STRUCTURE 4.51 kb     3 Exon(s)
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked N status provisional
Physical map
SUCLG1 2p11.2 succinate-CoA ligase, GDP-forming, alpha subunit FLJ37357 2p11.2 hypothetical protein FLJ37357 LOC200383 2p11.2 similar to Dynein heavy chain at 16F FLJ23529 2p11.2 hypothetical protein FLJ23529 LOC129293 2p11.2 hypothetical protein LOC129293 TMSB10 2p11.2 thymosin, beta 10 KCMF1 2p11.2 potassium channel modulatory factor 1 LOC391388 2 hypothetical gene supported by AJ238095; NM_014463 TCF7L1 2p11.2 transcription factor 7-like 1 (T-cell specific, HMG-box) LOC388966 2 similar to hypothetical protein FLJ20296 TGOLN2 2p11.2 trans-golgi network protein 2 LOC388967 2 LOC388967 LOC388968 2 similar to Phosphatidylethanolamine-binding protein (PEBP) (Prostatic binding protein) (HCNPpp) (Neuropolypeptide h3) (Raf kinase inhibitor protein) (RKIP) FLJ20296 2p11.2 hypothetical protein FLJ20296 FLJ21977 2p11.2 hypothetical protein FLJ21977 CAPG 2cen-q24 capping protein (actin filament), gelsolin-like LOC284948 2p11.2 similar to B-cell linker; B cell linker protein MAT2A 2p11.2 methionine adenosyltransferase II, alpha GGCX 2p12 gamma-glutamyl carboxylase VAMP8 2p11.2-p11.1 vesicle-associated membrane protein 8 (endobrevin) VAMP5 2p11.2 vesicle-associated membrane protein 5 (myobrevin) LOC51255 2p11.2 hypothetical protein LOC51255 FLJ90024 2p11.2 fasting-inducible integral membrane protein TM6P1 LOC388969 2 LOC388969 USP39 2p11.2 ubiquitin specific protease 39 SFTPB 2p12-p11.2 surfactant, pulmonary-associated protein B GNLY 2p12-q11 granulysin HATH6 SIAT9 2p11.2 sialyltransferase 9 (CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; GM3 synthase) POLR1A 2p11.2 polymerase (RNA) 1 polypeptide A, 194kDa FLJ20758 2p11.2 FLJ20758 protein IMMT 2p11.2 inner membrane protein, mitochondrial (mitofilin) MRPL35 2p11.2 mitochondrial ribosomal protein L35 FLJ13110 2p11.2 hypothetical protein FLJ13110 JMJD1 2p11.2 jumonji domain containing 1 NEDF 2p24.3-p24.1 neuroendocrine differentiation factor
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
3 - 757 11.3 100 - 2008 18253931
EXPRESSION
Type
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestivesalivary gland    
Reproductivefemale systembreastmammary gland  
Skin/Tegumentskin appendagesskin glandsebaceous gland  
 skin appendagesskin glandsweat gland  
Visualeyelacrimal gland   
cell lineage
cell lines
fluid/secretion salivary
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • an amphipathic helix which may participate in coiled coil interactions
  • a C terminal hydrophobic domain predicted to serve as a membrane anchor
  • HOMOLOGY
    intraspecies homolog to synaptobrevin
    Homologene
    FAMILY
  • synaptobrevin family
  • CATEGORY regulatory , transport
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endosome
    intracellular,cytoplasm,cytosolic,granule
    text
  • early endosomes, colocalizing with STX7
  • translocate to the plasma membrane and interact with SNAP23 and STX4 upon activation
  • basic FUNCTION
  • soluble N ethylmaleimide-sensitive factor-attachment protein receptor, SNARE protein
  • associated with the early endosome, involved in regulating membrane traffic
  • major vesicular SNARE (v-SNARE) of zymogen granules of pancreatic exocrine acinar cells
  • required with VAMP7 for activation-induced degranulation of mature mast cells
  • inhibits phagocytosis in dendritic cells
  • combinational SNARE proteins VAMP8 and VTI1B mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation
  • necessary for the endo-membrane fusion reactions that mediate the final stages of cytokinesis
  • plays a complex role in the control of granule secretion, transport vesicle trafficking, phagocytosis, and endocytosis
  • with other VAMPs, has differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs
  • SNARE first associated with endocytic processes but more recently has been suggested as an R-SNARE in regulated exocytosis
  • SNARE selectively required for granule-to-granule fusion during sequential exocytosis
  • key regulator of compound exocytosis in the exocrine pancreas
  • STX17, SNAP29, and VAMP8, are potentially essential for the fusion between autophagosomes and lysosomes
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS cellular trafficking transport
    PATHWAY
    metabolism
    signaling
    a component
  • complex between proteins on vesicle and target membranes
  • VAMP3 and VAMP8 forming SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion
  • STX8 belongs to the endosomal SNARE complex which also contains STX7, VTI1B and VAMP8
  • assembled core SNARE complex consisting of STX3, SNAP23 and VAMP8
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • soluble NSF-attachment protein (alpha-SNAP), STX7, NSF, RAB-GTPase
  • may interact with syntaxin 4 and SNAP23 (may act as a v-SNARE for regulated secretion of the entire exocrine system)
  • interacting with SLC2A4 (VAMP8 required for plasma membrane endocytosis)
  • interacting with SLC2A4 (in the absence of VAMP8, the relative subcellular distribution of SLC2A4 is altered, resulting in increased sarcolemma levels that can account for increased glucose clearance and insulin sensitivity)
  • syntaxin 3 associates with VAMP8
  • STX17 interacts with SNAP29 and the lysosomal SNARE VAMP8, and all of these proteins are required for autophagosome-lysosome fusion
  • ATG14 directly binds to the STX17-SNAP29 binary complex on autophagosomes and promotes STX17-SNAP29-VAMP8-mediated autophagosome fusion with lysosomes
  • cell & other
    REGULATION
    Other substrate of caspases, which thus regulate phagocytosis
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Susceptibility to early-onset myocardial infarction (MI)
    Variant & Polymorphism SNP associated with early-onset MI
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • VAMP8 null mice resulted in hydronephrosis and the level of AQP2 was increased by 3 to 5fold. VAMP8 may mediate the regulated fusion of AQP2-positive vesicles with the plasma membrane
  • VAMP8 null mice display reduced adiposity with increased energy expenditure despite normal food intake and reduced spontaneous locomotor activity