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FLASH GENE

Symbol

VAMP8

contributors: mct/pgu - updated : 20-06-2016

HGNC name	vesicle-associated membrane protein 8 (endobrevin)
HGNC id	12647

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	2p11.2      Physical location : 85.804.613 - 85.809.155
Synonym name	endobrevin
Synonym symbol(s)	EDB, VAMP-8

DNA

TYPE	functioning gene
STRUCTURE	4.51 kb     3 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_003761 3 - 757 NP_003752 11.3 100 - 2008 18253931

EXPRESSION

Type

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive salivary gland         Reproductive female system breast mammary gland     Skin/Tegument skin appendages skin gland sebaceous gland       skin appendages skin gland sweat gland     Visual eye lacrimal gland

cell lineage
cell lines
fluid/secretion	salivary

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	an amphipathic helix which may participate in coiled coil interactions
	a C terminal hydrophobic domain predicted to serve as a membrane anchor

HOMOLOGY

intraspecies

homolog to synaptobrevin

Homologene

FAMILY

synaptobrevin family

CATEGORY

regulatory , transport

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,endosome
	intracellular,cytoplasm,cytosolic,granule
text	early endosomes, colocalizing with STX7
	translocate to the plasma membrane and interact with SNAP23 and STX4 upon activation

basic FUNCTION	soluble N ethylmaleimide-sensitive factor-attachment protein receptor, SNARE protein
	associated with the early endosome, involved in regulating membrane traffic
	major vesicular SNARE (v-SNARE) of zymogen granules of pancreatic exocrine acinar cells
	required with VAMP7 for activation-induced degranulation of mature mast cells
	inhibits phagocytosis in dendritic cells
	combinational SNARE proteins VAMP8 and VTI1B mediate the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential event for autophagic degradation
	necessary for the endo-membrane fusion reactions that mediate the final stages of cytokinesis
	plays a complex role in the control of granule secretion, transport vesicle trafficking, phagocytosis, and endocytosis
	with other VAMPs, has differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs
	SNARE first associated with endocytic processes but more recently has been suggested as an R-SNARE in regulated exocytosis
	SNARE selectively required for granule-to-granule fusion during sequential exocytosis
	key regulator of compound exocytosis in the exocrine pancreas
	STX17, SNAP29, and VAMP8, are potentially essential for the fusion between autophagosomes and lysosomes

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

cellular trafficking transport

PATHWAY

metabolism

signaling

a component	complex between proteins on vesicle and target membranes
	VAMP3 and VAMP8 forming SNARE complexes with platelet syntaxin 4 and are required for platelet granule secretion
	STX8 belongs to the endosomal SNARE complex which also contains STX7, VTI1B and VAMP8
	assembled core SNARE complex consisting of STX3, SNAP23 and VAMP8

INTERACTION

DNA

RNA

small molecule

protein	soluble NSF-attachment protein (alpha-SNAP), STX7, NSF, RAB-GTPase
	may interact with syntaxin 4 and SNAP23 (may act as a v-SNARE for regulated secretion of the entire exocrine system)
	interacting with SLC2A4 (VAMP8 required for plasma membrane endocytosis)
	interacting with SLC2A4 (in the absence of VAMP8, the relative subcellular distribution of SLC2A4 is altered, resulting in increased sarcolemma levels that can account for increased glucose clearance and insulin sensitivity)
	syntaxin 3 associates with VAMP8
	STX17 interacts with SNAP29 and the lysosomal SNARE VAMP8, and all of these proteins are required for autophagosome-lysosome fusion
	ATG14 directly binds to the STX17-SNAP29 binary complex on autophagosomes and promotes STX17-SNAP29-VAMP8-mediated autophagosome fusion with lysosomes
	is dispensable for lysosome acidification, but promotes the completion of autophagy via promotion of autophagosome-lysosome fusion through its interaction with STX17 and VAMP8

cell & other

REGULATION

Other

substrate of caspases, which thus regulate phagocytosis

ASSOCIATED DISORDERS

corresponding disease(s)

Susceptibility

to early-onset myocardial infarction (MI)

Variant & Polymorphism SNP	associated with early-onset MI

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS

	VAMP8 null mice resulted in hydronephrosis and the level of AQP2 was increased by 3 to 5fold. VAMP8 may mediate the regulated fusion of AQP2-positive vesicles with the plasma membrane
	VAMP8 null mice display reduced adiposity with increased energy expenditure despite normal food intake and reduced spontaneous locomotor activity