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FLASH GENE

Symbol

VAMP2

contributors: mct/npt/pgu - updated : 30-06-2015

HGNC name	vesicle-associated membrane protein 2 (synaptobrevin 2)
HGNC id	12643

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	17p13.1      Physical location : 8.062.464 - 8.066.293
Synonym name	synaptobrevin 2
Synonym symbol(s)	SYB2, FLJ11460, VAMP-2

DNA

TYPE	functioning gene
STRUCTURE	3.83 kb     5 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

confirmed

Map	pter - D17S849 - D17S1828 - D17S1298 - D17S1537 - D17S938 - VAMP2 - D17S786 - cen
Authors	PMID: 10965122

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_014232 5 - 2173 NP_055047 - 116 - 2008 18182011

EXPRESSION

Type

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Nervous brain hindbrain pons trigeminal ganglia

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Muscular striatum skeletal

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic platelet

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	N-terminal half of the SNARE motif of VAMP2 serves as a sorting determinant for recognition by the related ANTH domain-containing endocytic adaptor
	C terminus with a potential role in fusion pore formation

secondary structure

one alpha helix contributing to the four helix bundle of synaptic SNARE complex

HOMOLOGY

Homologene

FAMILY

synaptobrevin family

CATEGORY

regulatory , structural protein , transport

SUBCELLULAR LOCALIZATION	plasma membrane
	intracellular
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,Golgi
	intracellular,cytoplasm,organelle,endosome
	intracellular,cytoplasm,cytosolic,granule
	intracellular,cytoplasm,cytosolic,vesicle
text	fusion of synaptic vesicles to target membranes
	VAMP1 and VAMP2, both co-sediment and co-localize with NPPA
	colocalized with CPLX2 along the apical plasma membrane following cholecystokinin-8 stimulation
	SEMA3A receptor and VAMP2 colocalize in endosomal membranes

basic FUNCTION	soluble N ethylmaleimide-sensitive factor-attachment protein receptor, SNARE protein
	calcium dependent role in the docking and fusion of synaptic vesicles with the presynaptic membrane
	role for both myosin Va and VAMP2 in oligodendrocyte function as it relates to myelination
	playing a central role in neural exocytosis
	regulate GLUT4 trafficking in slow-twitch myofibers in soleus muscle and neurotransmitter release in nerve endings
	plays roles in quiescent satellite cells and is involved in muscle regeneration
	VAMP2 mediates the trafficking of alpha5beta1 integrin to the plasma membrane and VAMP2-dependent integrin trafficking is critical in cell adhesion, migration and survival
	with VAMP1, and syntaxin-4 regulate NPPA release from cardiac myocyte
	with other VAMPs, has differential membrane fusion capacities, and imply that with the exception of VAMP5, VAMPs are essentially redundant in mediating fusion with plasma membrane t-SNAREs
	essential for T3 to increase insulin-stimulated translocation of SLC2A4 and subsequent uptake of glucose in adipocytes
	most abundant synaptic vesicle protein, required for fast calcium-triggered synaptic vesicle fusion
	SEMA3A-mediated signaling and axonal repulsion require VAMP2-dependent vesicular traffic

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

exocytosis transport

PATHWAY

metabolism

signaling

a component	constituent of a synaptic core complex with syntaxin and synaptosome associated proteins (SNAP25)
	critical component of the synaptic vesicle--fusion machinery
	three SNAREs, STX4, VAMP1 and VAMP2, form a SNARE complex inside cardiac myocytes

INTERACTION

DNA

RNA

small molecule	metal binding,
	Ca2+

protein	binding to t-SNARES, syntaxin (STXs) and SNAP25 after the fusion of synaptic vesicles to plasma membrane
	interacting with PRKD3 (may regulate VAMP2 vesicle trafficking by facilitating its recruitment to the target membrane)
	VAMP2 and RAB3A are SNARE proteins that interact with RABAC1
	VAMP2 is interaction partner of WDFY2 (with VAMP2, WDFY2 may be involved in vesicle cycling in various secretory pathway)
	association of VAMP2 with synaptophysin I in the specification of the pathway of synaptic vesicle biogenesis
	interacting with PICALM (facilitates the endocytosis of the synaptic vesicle protein VAMP2 from the plasma membrane)
	interacting with SLC2A4 (VAMP2 required for sorting to the specialized insulin-responsive compartment after plasma membrane endocytosis)
	interaction of STXBP4 with STX4 prevents interaction of VAMP2 located in SLC2A4 vesicle with STX4 in basal state
	interacts with SNAP-25 and syntaxin-1 to form a stable SNARE complex
	SEPT8 suppresses the interaction between VAMP2 and synaptophysin through binding to VAMP2
	modulates KCNB1 inactivation by interfering with the interaction between the docking loop and C1a, a mechanism for gating regulation that may pertain also to other Kv channels
	interacting with STXBP5 (tail domain of STXBP5 controlled membrane fusion though STXBP5 displacement by VAMP2)
	CPLX2 interact with vesicle-associated membrane protein (VAMP2), syntaxins 3 and 4, but not with VAMP8 or syntaxin 2
	PICALM controls the level of the synaptic vesicle protein VAMP2 at the plasma membrane by regulating VAMP2 endocytosis
	associated with Neuropilin 1 (NRP1) and PLXNA1, two essential components of the SEMA3A receptor, via its juxtatransmembrane domain

cell & other

targeted to renin-containing granules and mediates the stimulatory effect of cAMP on renin exocytosis

REGULATION

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS