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FLASH GENE
Symbol USO1 contributors: mct - updated : 12-10-2018
HGNC name USO1 homolog, vesicle docking protein (yeast)
HGNC id 30904
Location 4q21.1      Physical location : 76.649.828 - 76.735.364
Synonym name
  • transcytosis-associated protein
  • general vesicular transport factor p115
  • vesicle docking protein p115
  • Golgi vesicle tethering protein p115
  • Synonym symbol(s) TAP, P115, VDP
    DNA
    TYPE functioning gene
    STRUCTURE 89.77 kb     24 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    24 - 3998 108 962 - 2007 17274799
    26 - 4222 - 973 - 2007 17274799
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Endocrineparathyroid   highly
    Reproductivemale systemtestis  highly
    Respiratoryrespiratory tracttrachea  predominantly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectiveadipose  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • homologous region 2 (HR2) of the head domain that is highly homologous with the yeast counterpart, Uso1p
  • two globular "head" domains
  • an extended "tail" region reminiscent of the myosin-II structure
  • four sequential coil-coil domains distal to the globular head region
  • a C-terminal fragment (CTF) of 205 AAs generated by caspase cleavage (CTF transport into the nucleus was necessary to induce and accelerate apoptosis triggered by external stimuli)
  • mono polymer homomer , dimer
    HOMOLOGY
    Homologene
    FAMILY
  • VDP/USO1/YBL047C family
  • CATEGORY transport
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytosolic
    text cytosol (phosphorylated form) in interphase
    basic FUNCTION
  • general factor for binding vesicles to receptor membranes
  • RAB1 effector
  • vesicle-tethering protein functioning in endoplasmic reticulum-Golgi trafficking
  • plays an important role in the biogenesis and maintenance of the Golgi by interacting with the COG complex on the cis-Golgi in vesicular trafficking
  • acting directly, rather than via a tether, to catalyze trans-SNARE complex formation preceding membrane fusion
  • plays a key role in Golgi biogenesis and assembly
  • critical intermediary component in the regulated secretion of MIF from monocytes/macrophages
  • USO1 is indispensable for early embryonic development
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS endocytosis transport
    text intracisternal transport from the cis to the medial compartment of the Golgi apparatus
    PATHWAY
    metabolism
    signaling
    a component
  • Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • binding to the cis-Golgi GOLGA2 (GM130) protein and GOLGB1, both required for ER to Golgi traffic
  • interaction with COG2 (essential for Golgi ribbon reformation after the disruption of the ribbon by USO1 KD or brefeldin A treatment and recovery by re-expression of USO1 or drug wash out, respectively)
  • USO1 plays an important role in the biogenesis and maintenance of the Golgi by interacting with the COG complex (and COG2) on the cis-Golgi in vesicular trafficking
  • Golgi organization depends on mutually interacting domains in COPB2 and USO1, suggesting that vesicle tethering at the Golgi involves USO1 binding to the COPI coat
  • interaction with GOLGA2 and GOLGB1 (giantin) resulting in Golgi fragmentation but not apoptosis
  • MIF interacts with USO1 in the cytoplasm and the stimulated secretion of MIF results in the accumulation of both proteins in supernatants, which is consistent with MIF release from cells in conjunction with USO1
  • CREB3 was identified as a transcriptional regulator of upregulated ER-Golgi trafficking genes ARF4, COPB1, and USO1, and silencing of these genes attenuated lung colonization in breast cancer
  • cell & other
    REGULATION
    Other recruited by RAB1 to coat protein complex II (COPII) vesicles where it forms a CIS-SNARE complex that promotes targeting to the Golgi apparatus
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in multiple myeloma (MM) tissues and cells were much higher than those in normal bone marrow tissues and cells
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
  • may be a diagnosis marker in human multiple myeloma (MM)
  • Therapy target
    SystemTypeDisorderPubmed
    cancerhemopathy 
    may be a promising target for the therapy of human multiple myeloma (MM)
    ANIMAL & CELL MODELS