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FLASH GENE
Symbol UBE2I contributors: mct/npt/pgu - updated : 08-04-2016
HGNC name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
HGNC id 12485
Location 16p13.3      Physical location : 1.359.179 - 1.375.390
Synonym name
  • SUMO-1-protein ligase
  • ubiquitin carrier protein
  • ubiquitin conjugating enzyme 9
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • Synonym symbol(s) UBC9, UBCI, C358B7.1, P18, UBCE9
    EC.number 6.3.2.19
    DNA
    TYPE functioning gene
    STRUCTURE 17.87 kb     8 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    7 - 2871 18 158 - 1996 8668529
    8 - 3128 18 158 - 1996 8668529
    7 - 2991 18 158 - 1996 8668529
    7 - 2843 18 158 - 1996 8668529
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
     stomach   highly
    Endocrinethyroid   highly
    Lymphoid/Immunelymph node   highly
    Nervousbrain    
    Reproductivefemale systemovary  highly
    Respiratorylung    
    Visualeye     Homo sapiens
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectiveadipose    Homo sapiensAdult
    cells
    SystemCellPubmedSpeciesStageRna symbol
    not specificadipocyte Homo sapiensAdult
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period pregnancy
    Text placenta
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • conserved UBC domain of 150 AA harboring the cysteine residue required for enzyme-ubiquitin thioester formation
  • C-terminus involved in both the protein-protein interaction and its sumoylation-modifying activity provide the mechanism for regulating nuclear receptor functions
  • HOMOLOGY
    interspecies homolog to yeast UBC9
    homolog to S.Cerevisiae Scubc9
    Homologene
    FAMILY
  • ubiquitin-conjugating enzyme family
  • CATEGORY enzyme
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text
  • strikingly colocalized with SFRS2, which is a component of nuclear speckles and critical for mRNA processing
  • expressed predominantly in the nucleus of preadipocytes but shifted to cytoplasmic compartments
  • SAE1, UBE2I and PIAS1 are distributed in both nucleus and cytoplasm of ocular cell lines
  • basic FUNCTION
  • involved in ubiquitin conjugation,second step of ubiquitin-dependent proteolysis
  • SUMO-E2 enzyme, sufficient for substrate recognition and lysine modifications of SUMO targets
  • promoting nuclear accumulation and metabolic stability of tumor suppressor SMAD4
  • can function as a co-factor of PLAGL2, uncoupling from its enzymatic activity, to mediate PLAGL2 interactive surfactant protein-C promoter activity
  • essential gene which is required for cell cycle progression and is involved in the degradation of S phase and M phase cyclins
  • sufficient for substrate recognition and lysine modification of known SUMO targets
  • essential protein in mammalian cells with a predominantly nuclear localization
  • critical for mRNA processing, and having a possible function of sumoylation in gene expression
  • E2-conjugating enzyme that transfers the activated small ubiquitin-like modifier (SUMO) to protein substrates, and thus it has an important function in sumoylation-mediated cellular pathways
  • involved in protein modification through covalent attachment of small ubiquitin-like modifier and, has emerged as a key regulator of cell proliferation and differentiation
  • appears to play an important role in adipogenesis
  • negatively regulates osteoblastic differentiation induced by BMP via, at least in part, SUMOylation of SMAD4
  • UBE2I acts as a functional binding partner of FYB1 and plays a selective role in integrin-mediated T cell adhesion via modulation of RAP1A-RASSF5 membrane recruitment and RAC1 activation
  • CELLULAR PROCESS cell cycle
    nucleotide, repair
    protein, degradation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • UBE2I associated to FHIT
  • RANBP2 forms a stable complex with SUMO-modified RANGAP1 and UBE2I at the nuclear pore complex
  • member of the surfactant protein-C promoter complex
  • IPO13ľUBE2I9 complex forms in the cytosol and translocates into the nucleus
  • non-covalent ternary complex formed by PIAS1, SUMO1, and UBE2I proteins involved in transcriptional regulation
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • associating with RAD51, RAD52 proteins in the double strand RAD51/RAD52 repair pathway
  • associating with UBL1 and TP53, involved in DNA recombination and essential for cell-cycle progression
  • associating with FHIT for cell-cycle control
  • interacting with ETV6 leading to sumoylation of ETV6 (TEL) by UBL (SUMO-1)
  • interacted with the DNA-binding and ligand-binding domains of ESR1
  • protein interacting with UIMC1
  • interacted with SOX4 through HMG-box domain of SOX4 and represses its transcriptional activity independent of its SUMO-1-conjugating activity
  • interacting with STAR and FOXL2 (UBE2I-mediated sumoylation at lysine 25 of FOXL2 is required for transcriptional repression of the STAR gene and may be responsible for controlling the development of ovarian follicles)
  • ZMYND11 interacts with PIAS1 (a well-characterized SUMO E3 enzyme) and UBE2I (the only SUMO E2 enzyme so far identified) through its distinct regions
  • molecular link between UBE2I and the metastasis genes such as CDC42 and CXCR4, and thus provide new insight into the mechanism by which UBE2I9 promotes tumor invasion and metastasis
  • binding of BRCA1 proteins to nuclear chaperone UE2I provides a novel mechanism for nuclear import and control of tumor growth
  • is an interaction partner of the transcription factor EGR2, a key regulator of hindbrain development
  • SETD8 interacts with the UBE2I E2 SUMO conjugating enzyme (directly binds SETD8 proximal to the catalytic SET domain)
  • RANBP2 forms a stable complex with SUMO-modified RANGAP1 and UBE2I at the nuclear pore complex
  • SKI can enhance UBE2I-mediated cellular sumoylation, possibly through direct interaction with UBE2I
  • interaction between the CANX cytoplasmic domain and UBE2I, a SUMOylation E2 ligase, which modified the calnexin cytoplasmic domain by the addition of SUMO
  • UBE2I interacted directly with FYB1 and the association was increased in response to anti-CD3 stimulation
  • cell & other
    REGULATION
    Other autosumoylation of the mammalian E2-conjugating enzyme UBE2I at Lys14 regulates target discrimination
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in advanced melanomas
    Susceptibility to late-onset Alzheimer disease (AD)
    Variant & Polymorphism other UBE2I polymorphisms might be associated with a risk of AD
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancerskin 
    blocking expression of UBE2I sensitizes melanomas to the cytotoxic effects of chemotherapeutic drugs
    ANIMAL & CELL MODELS