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FLASH GENE
Symbol UBE2I contributors: mct/npt/pgu - updated : 08-04-2014
HGNC name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
HGNC id 12485
Location 16p13.3      Physical location : 1.359.179 - 1.375.390
Synonym name
  • SUMO-1-protein ligase
  • ubiquitin carrier protein
  • ubiquitin conjugating enzyme 9
  • ubiquitin-like protein SUMO-1 conjugating enzyme
  • Synonym symbol(s) UBC9, UBCI, C358B7.1, P18, UBCE9
    EC.number 6.3.2.19
    DNA
    TYPE functioning gene
    STRUCTURE 17.87 kb     8 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    Physical map
    MGC2601 16p13.3 hypothetical protein MGC2601 MGC2494 16p13.3 hypothetical protein MGC2494 FLJ34512 16p13.3 hypothetical protein FLJ34512 HAGHL 16p13.3 hydroxyacyl glutathione hydrolase-like NARFL 16p13.3 nuclear prelamin A recognition factor-like MSLN 16p13.3 mesothelin RLUCL CHTF18 16p13.3 CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) GNG13 16p13.3 guanine nucleotide binding protein (G protein), gamma 13 LOC388199 16 LOC388199 FLJ12681 16p13.3 hypothetical protein FLJ12681 SOX8 16p13.33 SRY (sex determining region Y)-box 8 FLJ32252 16p13.3 hypothetical protein FLJ32252 SSTR5 16p13.3 somatostatin receptor 5 LOC390663 16 similar to Williams-Beuren syndrome critical region protein 25 LOC390664 16 similar to C1q Related CACNA1H 16p13.3 calcium channel, voltage-dependent, alpha 1H subunit TPS1 16p13.3 tryptase, alpha TPSB2 16p13.3 tryptase beta 2 TPSG1 16p13.3 tryptase gamma 1 TPSB1 16p13.3 tryptase beta 1 TPSD1 16p13.3 tryptase delta 1 ISP2 16p13.3 implantation serine proteinase 2 LOC123788 16p13.3 similar to mast cell protease-11 UBE2I 16p13.3 ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) BAIAP3 16p13.3 BAI1-associated protein 3 LOC388200 16 LOC388200 CAB56184 FLJ23360 16p13.3 hypothetical protein FLJ23360 UNKL 16p13.3 unkempt-like (Drosophila) LOC283951 16p13.3 hypothetical protein LOC283951 LOC388201 16 LOC388201 LOC388202 16 similar to KIAA0445 protein CLCN7 16p13.3 chloride channel 7 LOC390666 16 similar to ribosomal protein S3a; 40S ribosomal protein S3a; v-fos transformation effector protein 1 LOC390667 16 similar to Neuronal pentraxin II precursor (NP-II) (NP2) KIAA0683 16p13.3 similar to Neuronal pentraxin II precursor (NP-II) (NP2) KIAA0590 16p13.3 similar to Neuronal pentraxin II precursor (NP-II) (NP2) FLJ20898 16p13.3 hypothetical protein FLJ20898 CRAMP1L 16p13.3 Crm, cramped-like (Drosophila) C16orf34 16p13.3 chromosome 16 open reading frame 34 MAPK8IP3 16p13.3 mitogen-activated protein kinase 8 interacting protein 3
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    7 - 2871 18 158 - 1996 8668529
    8 - 3128 18 158 - 1996 8668529
    7 - 2991 18 158 - 1996 8668529
    7 - 2843 18 158 - 1996 8668529
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
     stomach   highly
    Endocrinepancreas    
     thyroid   highly
    Lymphoid/Immunelymph node   highly
    Nervousbrain    
    Reproductivefemale systemovary  highly
    Respiratorylung    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectiveadipose    Homo sapiensAdult
    cells
    SystemCellPubmedSpeciesStageRna symbol
    not specificadipocyte Homo sapiensAdult
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period pregnancy
    Text placenta
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • conserved UBC domain of 150 AA harboring the cysteine residue required for enzyme-ubiquitin thioester formation
  • C-terminus involved in both the protein-protein interaction and its sumoylation-modifying activity provide the mechanism for regulating nuclear receptor functions
  • HOMOLOGY
    interspecies homolog to yeast UBC9
    homolog to S.Cerevisiae Scubc9
    Homologene
    FAMILY
  • ubiquitin-conjugating enzyme family
  • CATEGORY enzyme
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text
  • strikingly colocalized with SFRS2, which is a component of nuclear speckles and critical for mRNA processing
  • expressed predominantly in the nucleus of preadipocytes but shifted to cytoplasmic compartments
  • basic FUNCTION
  • involved in ubiquitin conjugation,second step of ubiquitin-dependent proteolysis
  • SUMO-E2 enzyme, sufficient for substrate recognition and lysine modifications of SUMO targets
  • promoting nuclear accumulation and metabolic stability of tumor suppressor SMAD4
  • can function as a co-factor of PLAGL2, uncoupling from its enzymatic activity, to mediate PLAGL2 interactive surfactant protein-C promoter activity
  • essential gene which is required for cell cycle progression and is involved in the degradation of S phase and M phase cyclins
  • sufficient for substrate recognition and lysine modification of known SUMO targets
  • essential protein in mammalian cells with a predominantly nuclear localization
  • critical for mRNA processing, and having a possible function of sumoylation in gene expression
  • E2-conjugating enzyme that transfers the activated small ubiquitin-like modifier (SUMO) to protein substrates, and thus it has an important function in sumoylation-mediated cellular pathways
  • involved in protein modification through covalent attachment of small ubiquitin-like modifier and, has emerged as a key regulator of cell proliferation and differentiation
  • appears to play an important role in adipogenesis
  • negatively regulates osteoblastic differentiation induced by BMP via, at least in part, SUMOylation of SMAD4
  • CELLULAR PROCESS cell cycle
    nucleotide, repair
    protein, degradation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • UBE2I associated to FHIT
  • RANBP2 forms a stable complex with SUMO-modified RANGAP1 and UBE2I at the nuclear pore complex
  • member of the surfactant protein-C promoter complex
  • IPO13ľUBE2I9 complex forms in the cytosol and translocates into the nucleus
  • non-covalent ternary complex formed by PIAS1, SUMO1, and UBE2I proteins involved in transcriptional regulation
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • associating with RAD51, RAD52 proteins in the double strand RAD51/RAD52 repair pathway
  • associating with UBL1 and TP53, involved in DNA recombination and essential for cell-cycle progression
  • associating with FHIT for cell-cycle control
  • interacting with ETV6 leading to sumoylation of ETV6 (TEL) by UBL (SUMO-1)
  • interacted with the DNA-binding and ligand-binding domains of ESR1
  • protein interacting with UIMC1
  • interacted with SOX4 through HMG-box domain of SOX4 and represses its transcriptional activity independent of its SUMO-1-conjugating activity
  • interacting with STAR and FOXL2 (UBE2I-mediated sumoylation at lysine 25 of FOXL2 is required for transcriptional repression of the STAR gene and may be responsible for controlling the development of ovarian follicles)
  • ZMYND11 interacts with PIAS1 (a well-characterized SUMO E3 enzyme) and UBE2I (the only SUMO E2 enzyme so far identified) through its distinct regions
  • molecular link between UBE2I and the metastasis genes such as CDC42 and CXCR4, and thus provide new insight into the mechanism by which UBE2I9 promotes tumor invasion and metastasis
  • binding of BRCA1 proteins to nuclear chaperone UE2I provides a novel mechanism for nuclear import and control of tumor growth
  • is an interaction partner of the transcription factor EGR2, a key regulator of hindbrain development
  • SETD8 interacts with the UBE2I E2 SUMO conjugating enzyme (directly binds SETD8 proximal to the catalytic SET domain)
  • RANBP2 forms a stable complex with SUMO-modified RANGAP1 and UBE2I at the nuclear pore complex
  • SKI can enhance UBE2I-mediated cellular sumoylation, possibly through direct interaction with UBE2I
  • interaction between the CANX cytoplasmic domain and UBE2I, a SUMOylation E2 ligase, which modified the calnexin cytoplasmic domain by the addition of SUMO
  • cell & other
    REGULATION
    Other autosumoylation of the mammalian E2-conjugating enzyme UBE2I at Lys14 regulates target discrimination
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in advanced melanomas
    Susceptibility to late-onset Alzheimer disease (AD)
    Variant & Polymorphism other UBE2I polymorphisms might be associated with a risk of AD
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancerskin 
    blocking expression of UBE2I sensitizes melanomas to the cytotoxic effects of chemotherapeutic drugs
    ANIMAL & CELL MODELS