Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
Symbol U2AF2 contributors: mct/npt/pgu - updated : 01-06-2015
HGNC name U2 small nuclear RNA auxiliary factor 2
HGNC id 23156
Location 19q13.42      Physical location : 56.165.415 - 56.186.081
Synonym name
  • splicing factor U2AF 65 kD subunit
  • U2 snRNP auxiliary factor large subunit
  • U2 (RNU2) small nuclear RNA auxiliary factor 2
  • U2 small nuclear ribonucleoprotein auxiliary factor (65kD)
  • Synonym symbol(s) U2AF65
    TYPE functioning gene
    STRUCTURE 20.67 kb     12 Exon(s)
    MAPPING cloned Y linked N status confirmed
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    12 splicing 3148 53 475 - 2009 18974054
    12 splicing 3136 53 471 - 2009 18974054
  • using an alternate in-frame splice site compared to variant 1
  • having the same N- and C-termini compared to variant 1
    Type ubiquitous
       expressed in (based on citations)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivepharynx   highly
     salivary gland   highly
    Endocrineadrenal gland   moderately
    Lymphoid/Immunelymph node   highly
    Reproductivemale systemprostate  moderately
    Respiratoryrespiratory tractlarynx  moderately
    Visualeye   moderately
    cell lineage
    cell lines
    fluid/secretion moderately in lymph
    at STAGE
  • three RNA recognition motifs (RRM), tandem RNA recognition motifs recognizing polypyrimidine tract signals adjacent to 3 prime splice sites , tandem RRM domains that do not simply act as a binding scaffold but instead have an active role in quantitatively relating Py tract strength to splice site recognition and spliceosome assembly
  • an arg/ser rich domain
  • mono polymer heteromer , dimer , complex
    interspecies ortholog to rattus U2af2 (100pc)
    homolog to drosophila U2af50 (74.9pc)
    ortholog to C. elegans uaf-1 (62.3pc)
  • SR family of splicing factor
  • CATEGORY RNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    text distributed throughout the nucleoplasm, where transcription occurs, with additional concentration in nuclear speckles, where splicing factors accumulate when not engaged in splicing
    basic FUNCTION
  • necessary for the splicing of pre-mRNA
  • required for the export of mRNA out of the nucleus
  • increasing the affinity of SF1 for BPS
  • promoting 3'-end processing, which contributes to 3'-terminal exon definition
  • SF1 and U2AF2 associate cooperatively with pre-mRNA and play a crucial role in 3 prime splice site recognition during early steps of spliceosome assembly
  • essential pre-mRNA splicing factor for the initial stages of spliceosome assembly
  • has a crucial role in the assembly of splicing complexes
  • modulating the function of the PLE (poly(A)-limiting element)
  • essential splicing factor in the recognition of the pre-mRNA 3' splice sites during the assembly of the splicing commitment complex
  • SF1 and U2 auxiliary factor (U2AF2) cooperatively recognize the 3 prime splice site during the initial stages of pre-mRNA splicing
  • involved in the nuclear export of mRNA
  • both U2AF2 and PRPF19 are required for RNA polymerase II C-terminal domain-dependent splicing activation
  • served as an adaptor to link expanded CAG RNA to NXF1 for RNA export
  • plays a key role in regulating the nuclear export of expanded CAG RNA, and its temporal reduction in levels causes accumulation of expanded CAG RNA in the cell nucleus
  • the essential splicing factors U2AF2 and SF1 cooperatively bind consensus sequences at the 3' end of introns
  • plays a critical role in regulating the level of TERF1 through physical interaction and ubiquitin-mediated proteolysis
  • intimate link between JMJD6 and U2AF2 in alternative splicing regulation, which has important implications in development and disease processes
  • CELLULAR PROCESS nucleotide, RNA splicing
    a component
  • heterodimerizing with U2AF1 to form the essential splicing factor U2AF (U2 auxiliary factor)
  • U2 snRNP auxiliary factor (U2AF) is an essential splicing factor composed of two subunits, a large, 65-kDa subunit (U2AF(65)) and a small subunit, U2AF(35)
  • direct binding of SF1 to U2AF2 in both the nucleoplasm
  • and nuclear speckles
  • binding to the polypyrimidine tract of introns during spliceosome assembly
  • directly interacts with expanded CAG RNA via its RRM3 domain
  • small molecule
  • unphosphorylated SF1 via its N terminus
  • interacting with PUF60 (mutually enhance their affinity for binding polypyrimidine tract RNA in a cooperative fashion)
  • compete with MBNL1 by binding to mutually exclusive RNA structures (binds the same region in a single-strand structure)
  • RBM39 and ZC3H11A interacting wth U2AF2
  • binding of U2AF2 to a polypyrimidine tract requires a flexible RNA backbone
  • JMJD6 mediates splicing of FLT1 by interacting with U2AF2
  • facilitates RNA export through its interaction with the export receptor NXF1
  • interaction of ATXN1 with the splicing factors RBM17 and U2AF2
  • ZRSR2 physically interacts with U2AF2, as well as SRSF1 and SRSF2, with a distinct function from its homologue, U2AF1
  • essential pre-mRNA splicing factor, and a novel TERF1-interacting protein
  • HNRNPM promotes exon 7 inclusion of SMN1 and SMN2 pre-mRNA through targeting an enhancer on exon 7 through recruiting U2AF2
  • PRPF40B interacts directly with SF1 and associates with U2AF2
  • U2AF2 interacts with SF1 and was shown to recruit the U2 snRNP to the spliceosome
  • interacts with and hydroxylates multiple serine/arginine-rich (SR) proteins and SR related proteins, including U2AF2
  • cell & other
    Other proteolyzed during apoptosis (N-terminal fragment mainly accumulates in the nucleus within nuclear bodies and to a much lesser extent in the cytoplasm, whereas the C-terminal fragment is found in the cytoplasm, even in localization studies on apoptosis induction)
    post-translational hydroxylation catalyzed by JMJD6
    corresponding disease(s)
    Variant & Polymorphism
    Candidate gene
    Therapy target
  • knockdown of U2af50 (the Drosophila ortholog of U2AF2) expression intensified the accumulation of expanded CAG RNA in the nucleus and exacerbated RNA-mediated toxicity