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FLASH GENE
Symbol SPIRE1 contributors: mct/ - updated : 01-03-2014
HGNC name spire homolog 1 (Drosophila)
HGNC id 30622
Location 18p11.21      Physical location : 12.446.511 - 12.657.912
Synonym name protein spire homolog 1
Synonym symbol(s) Spir-1, KIAA1135, MGC150622, MGC150621, SPIR1
DNA
TYPE functioning gene
STRUCTURE 214.98 kb     17 Exon(s)
MAPPING cloned Y linked N status provisional
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
16 - 5402 83.8 742 - 2011 21705804
17 - 5444 85.4 756 - 2011 21705804
16 - 5100 71.4 622 localizes to mitochondria 2011 21705804
  • SPIRE1C
  • directly links mitochondria to the actin cytoskeleton and the ER
  • binds INF2 and promotes actin assembly on mitochondrial surfaces (PMID: 26305500)
  • EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivepancreas exocrine   moderately
    Nervousbrain   moderately
    Reproductivefemale systemuteruscervix moderately
    Respiratorylung   predominantly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectivebone   
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES acid
    STRUCTURE
    motifs/domains
  • a SPIR kinase non-catalytic C-lobe domain (KIND)
  • an acidic domain
  • four tandem WH2 domains, playing a role in filament nucleation by Arp2/3 complex, and is capable of preventing both profilin-actin
  • addition to filaments and barbed end depolymerization with nanomolar potency
  • a zinc finger structure
  • a C-terminal LxL motif, which is a docking site for ERK and JNK, and C-terminal formin Spir interaction motif (FSI)
  • HOMOLOGY
    interspecies ortholog to murine Spire1
    Homologene
    FAMILY
  • family of Wiskott-Aldrich homology region-2 (WH2) proteins
  • spire family
  • CATEGORY regulatory , transport
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytosolic,vesicle
    intracellular,cytoplasm,cytoskeleton
    basic FUNCTION
  • involved in actin organization and in filament nucleation
  • involved in vesicle transport processes
  • SPIRE1, SPIRE2, and COBL can synergize with other actin regulators
  • mediated actin-microtubule interactions and has important roles in membrane transport (Ducka 2010)
  • SPIRE1 and SPIRE2 cooperate with Formin-2 (FMN2) to nucleate actin filaments
  • COBL and SPIRE1, SPIRE2 display different functions by selecting different sets of the multifunctional properties of WH2 domains
  • in mammalian oocytes, three actin binding proteins, FMN2, SPIRE1, and profilin, synergistically organize a dynamic cytoplasmic actin meshwork that mediates translocation of the spindle toward the cortex and is required for successful fertilization
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS exocytosis transport , endocytosis transport , cellular trafficking transport
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • actin (one monomer by WASP homology 2 (WH2) domain)
  • SPIRE1, SPIRE2 cooperate with FMN2 to drive asymmetric oocyte division
  • recruits FMN2 and facilitates its association with barbed ends, followed by rapid processive assembly and release of SPIRE1
  • cooperates with INF2 to regulate actin assembly at ER-mitochondrial contacts
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS