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FLASH GENE
Symbol SPAG5 contributors: mct/npt/pgu - updated : 25-04-2012
HGNC name sperm associated antigen 5
HGNC id 13452
Location 17q11.2      Physical location : 26.904.584 - 26.926.056
Synonym name
  • astrin
  • mitotic spindle coiled-coil related protein
  • mitotic spindle associated protein p126
  • Synonym symbol(s) MAP126, DEEPEST, astrin
    DNA
    TYPE functioning gene
    STRUCTURE 21.47 kb     24 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    Physical map
    NLK 17q11.2 nemo-like kinase PYY2 17q11.1-q11.2 peptide YY, 2 (seminalplasmin) PPY2 17q11.1-q11.2 pancreatic polypeptide 2 LOC339274 17q11.2 similar to Hypothetical protein KIAA0563 FLJ40504 17q11.2 hypothetical protein FLJ40504 MAC30 17q11.2 hypothetical protein FLJ40504 LOC90410 17q11.2 intraflagellar transport protein IFT20 TNFAIP1 17q11 tumor necrosis factor, alpha-induced protein 1 (endothelial) PDIP38 MGC45714 17q11.2 hypothetical protein MGC45714 VTN 17q11.2 vitronectin (serum spreading factor, somatomedin B, complement S-protein) SARM1 17q11 sterile alpha and TIR motif containing 1 MGC9564 17q11.2 similar to RIKEN cDNA 1110002C08 gene LOC388363 17 similar to 40S ribosomal protein S7 (S8) SLC13A2 17p11.1-q11.1 solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2 FOXN1 17q11.2 forkhead box N1 UNC119 17q11.2 unc-119 homolog (C. elegans) PIGS 17p13.2 phosphatidylinositol glycan, class S ALDOC 17q11.2 aldolase C, fructose-bisphosphate SPAG5 17q11.1 sperm associated antigen 5 FLJ25006 17q11.2 hypothetical protein FLJ25006 KIAA0100 17q11.2 KIAA0100 gene product SDF2 17q11.2 stromal cell-derived factor 2 SUPT6H 17q11.2 suppressor of Ty 6 homolog (S. cerevisiae) MGC39650 17q11.2 hypothetical protein MGC39650 RAB34 17q11.1 RAB34, member RAS oncogene family RPL23A 17q11 ribosomal protein L23a LOC116238 17q11.2 hypothetical protein BC014072 NEK8 17q11.1 NIMA (never in mitosis gene a)- related kinase 8 TRAF4 17q11-q12 TNF receptor-associated factor 4 FLJ10700 17q11.2 hypothetical protein FLJ10700 ERAL1 17q11.2 Era G-protein-like 1 (E. coli) FLOT2 17q11.2-q12 flotillin 2 MGC23280 17q11.2 hypothetical protein MGC23280 PHF12 17q11.2 PHD finger protein 12 SEZ6 17q11.2 seizure related 6 homolog (mouse) LOC342659 17q11.2 similar to NADH-ubiquinone oxidoreductase 15 kDa subunit (Complex I-15 kDa) (CI-15 kDa)
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    24 - 3824 134.3 1193 - 2008 18361916
    EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivemouth   moderately
    Lymphoid/Immunethymus   moderately
    Reproductivefemale systemuteruscervix highly
     female systembreastmammary gland moderately
     male systemtestis  predominantlyHomo sapiens
    Skin/Tegumentskin   moderately
    cell lineage
    cell lines
    fluid/secretion moderately in lymph
    at STAGE
    physiological period fetal
    Text highly in umbilical cord
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • myosin tail
  • mono polymer homomer , dimer , oligo
    HOMOLOGY
    interspecies homolog to rattus Spag5 (67.6 pc)
    homolog to murine Spag5 (68 pc)
    Homologene
    FAMILY
    CATEGORY regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • condensed chromosome kinetochore
  • mainly associated with the meiotic spindle microtubules and concentrated on spindle poles at metaphase I and metaphase II stages
  • localizes to spindle poles and kinetochores in mammalian cells, yet its kinetochore localization is exclusive to chromosomes that have congressed to the metaphase plate and is absent on those that have not yet congressed
  • basic FUNCTION
  • involved in the functional and dynamic regulation of mitotic spindles
  • being necessary for normal spindle formation during mitosis
  • regulating mitotic spindle localization of AURKA
  • microtubule and kinetochore protein that may contribute to the regulatory network that controls separase activity
  • DCLRE1B and SPAG5 function together to enforce the prophase checkpoint in response to spindle stress
  • microtubule and kinetochore protein required for the maintenance of sister chromatid cohesion and centrosome integrity in human mitosis
  • stabilizes microtubules at the outer kinetochore
  • KIF2C, SPAG5 and KIF2B form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity
  • mitotic spindle-associated protein required for the correct alignment of all chromosomes at the metaphase plate
  • CELLULAR PROCESS cell cycle, division, mitosis
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    phosphoinositide-mediated signaling
    a component
  • homodimer with a globular head domain and a long stalk
  • homo-oligomer (the globular head domains associate, resulting in aster-like structures)
  • interacting with NIN (NIN regulates the dynamic movement of SPAG5 throughout the cell cycle and this interaction, in turn, is required for maintenance of centrosome/spindle pole integrity)
  • astrin-kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment
  • likely SPAG5-SKAP complex acts together with the NDC80 complex to stabilize correctly formed kinetochore-microtubule interactions
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with AURKA
  • interacts and co-localizes with NIN at the centrosome during the S and G2 phases, and this complex may dissociate in the M phase
  • GSK3beta interacts with SPAG5 (SPAG5 association with the spindle microtubule and kinetochore may be dependent on phosphorylation by GSK3beta)
  • DCLRE1B interacting with SPAG5, through its conserved metallo-beta-lactamase domain, and disruption of this interaction by point mutations results in a deficient prophase checkpoint
  • may interact with the centrosomal proteins Aurora-A or PLK1 to regulate microtubule organization and spindle pole integrity
  • physically associates with KIF2B and KIF2C
  • CDK5RAP2 interacts with SPAG5 and CEP72
  • cell & other
  • binding to microtubules in the mitotic spindle
  • targeting of the SPAG5-SKAP complex to bioriented kinetochores, controled by AURKB
  • REGULATION
    Phosphorylated by GSK3beta, that interacts with and phosphorylates the spindle-associated protein SPAG5, resulting in targeting SPAG5 to the spindle microtubules and kinetochores
    Other phosphorylated upon DNA damage, probably by ATM or ATR
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS