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FLASH GENE

Symbol

RPS3

contributors: mct - updated : 14-02-2014

HGNC name	ribosomal protein S3
HGNC id	10420

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	11q13.4      Physical location : 75.110.561 - 75.116.730
Synonym name	40S ribosomal protein S3, 26kDa, see RNU15A
Synonym symbol(s)	FLJ26283, FLJ27450, MGC87870

DNA

TYPE	functioning gene
SPECIAL FEATURE
text	cotranscribed with RNU15A, RNU15B, embedded in introns 1 and 5, and RNU14
STRUCTURE	6.17 kb     7 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

confirmed

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_001005 7 - 855 NP_000996 26 243 - 2004 14988002

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive intestine large intestine colon   moderately Lymphoid/Immune lymph node       highly   tonsils       highly Reproductive female system uterus cervix   highly   female system ovary     highly Skin/Tegument skin       highly

cell lineage

cell lines

fluid/secretion

at STAGE

physiological period

embryo

Text	kidney, umbilical cord, embryonic tissue

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	a KH type-2 domain
	C terminal domain, the major mRNA binding domain of RPS3, independent of the KH domain

HOMOLOGY

interspecies	homolog to rattus Rps3 (99.59 pc)
	homolog to murine Rps3 (99.59 pc)

Homologene

FAMILY

ribosomal protein S3P family

CATEGORY

enzyme , RNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytosolic,ribosome
	intracellular,nucleus,nucleolus
text	dynamic protein that shuttles between the cytoplasm and the nucleus and functions in both compartments
	nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival

basic FUNCTION	40S ribosomal protein involved in the repair of
	oxidative DNA lesions
	having an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage
	involved in induction of apoptosis
	functioning as a ribosomal protein but, in addition, may influence repair activities at sites of DNA damage
	plays a role in repairing various DNA damage acting as a repair UV endonuclease
	free RPS3 can regulates its own translation via a feedback mechanism
	protecting against UV-induced skin cell death
	ribosomal protein of the 40 S subunit, required for ribosome biogenesis extraribosomal
	role of RPS3 as a molecular switch that accommodates apoptotic induction to DNA repair through Akt-mediated phosphorylation
	may act upstream of caspase-3, most likely in the mitochondrial apoptotic pathway
	RPS3 is recruited to the DISC and plays a critical role in both genotoxic stress and cytokine induced apoptosis
	has multi-functions such as translation, DNA repair and apoptosis
	acts as a microtubule associated protein (MAP) and regulates spindle dynamics during mitosis

CELLULAR PROCESS	cell life, cell death/apoptosis
	nucleotide, repair
	nucleotide, genomic integrity

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	of the ribosome
	component of NFKppaB complex, involved in the regulation of key genes

INTERACTION

DNA

RNA

binding

small molecule

protein	multi-functional protein that interacts with both TP53 and MDM2 through its KH domain, acted as a scaffold for the additional binding of MDM2 and TP53, suggesting that it interacts with important proteins involved in maintaining genomic integrity (MID: 19656744)
	physiological target of AKT1 kinase and a novel mediator of neuronal apoptosis (AKT1-dependent phosphorylation of RPS3 inhibited its proapoptotic function and perturbed its interaction with E2F1)
	RPS3 appears to be recruited to the death-inducing signaling complex (DISC) to induce apoptosis by interacting TRADD in response to extracellular stresses

cell & other

REGULATION

Other	AKT1 phosphorylation governs RPS3 nuclear accumulation that in turn impairs its apoptotic function thereby promoting cell survival (
	arginine methylation of RPS3 plays a critical role in its import into the nucleolus, as well as in small subunit assembly of the ribosome

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in colorectal cancer

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed neurology neurodegenerative   phosphorylation by AKT1 may provide useful clues in the development of novel treatments for neurological diseases

ANIMAL & CELL MODELS