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FLASH GENE
Symbol RPA2 contributors: mct/pgu - updated : 02-03-2012
HGNC name replication protein A2, 32kDa
HGNC id 10290
Location 1p35.3      Physical location : 28.218.048 - 28.241.236
Synonym name
  • replication protein A, subunit 2
  • RF-A protein 2
  • Synonym symbol(s) REPA2, RPA32, RPA34, RP-A p32
    DNA
    TYPE functioning gene
    STRUCTURE 23.19 kb     9 Exon(s)
    Genomic sequence alignment details
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    9 - 1741 - 270 - 1999 10449415
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivesalivary gland   highly
    Endocrineparathyroid   highly
    Reproductivefemale systemovary  highly
    cell lineage spermatocytes
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a phosphorylated N terminal domain,
  • a central ssDNA-binding domain, and a DNA-binding domain DBD-D
  • a C terminal protein interaction domain, mediating interaction with XPA
  • HOMOLOGY
    Homologene
    FAMILY replication protein A family
    CATEGORY DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • colocalizing with RAD51 on synapsed axes in meiosis and facilitating RAD51 function, also colocalizing with BLM in meiotic prophase nuclei of spermatocytes
  • RPA2 phosphorylation facilitates chromosomal DNA repair
  • its hyperphosphorylation plays a critical role in maintenance of genomic stability and cell survival after a DNA replication block via promotion of homologous recombination
  • is an exchange point for multiple proteins involved in DNA repair, homologous recombination, and checkpoint processes and that it binds to different partners with comparable binding affinity using a single site
  • with RPA1, and RPA3, plays essential roles in DNA transactions
  • RPA1 and RPA2 are arranged from 5prime to 3prime along the unfolded telomeric G4, as already described for unstructured single-stranded DNA
  • CELLULAR PROCESS nucleotide, replication
    nucleotide, repair, nucleotide excision repair
    nucleotide, repair, recombination
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • component 70kDa of a trimeric single strand binding protein, the replication protein A complex, involved in replication, homologous recombination and nucleotide excision repair
  • MEIOB forms a complex with RPA2 and with SPATA22, and these three proteins co-localize in foci that are associated with meiotic chromosome
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • PPP4C dephosphorylates RPA2 to facilitate DNA repair via homologous recombination
  • RPA2 also interacts with TIPIN during the intra-S checkpoint
  • interacts with the specific proteins among those that play roles in the regulation of the replication fork progression
  • RFWD3 and RPA2 functionally interact and participate in replication checkpoint control
  • cell & other
    REGULATION
    Phosphorylated by RPA2 hyperphosphorylation by DNA-PK in response to double-strand breaks blocks unscheduled homologous recombination and delays mitotic entry
    ATR (in response to UV-induced DNA damage, ATR rapidly phosphorylates RPA2, disrupting its association with replication centers in the S-phase and contributing to the inhibition of DNA replication)
    Other dephosphorylated by the serine/threonine protein phosphatase 2A (PPP2R4)(PPP2R4-mediated RPA2 dephosphorylation is required for the efficient DNA damage repair)
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS