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FLASH GENE
Symbol RBX1 contributors: mct - updated : 10-11-2101
HGNC name ring-box 1, E3 ubiquitin protein ligase
HGNC id 9928
Location 22q13.2      Physical location : 41.347.384 - 41.368.666
Synonym name
  • ring-box 1
  • regulator of cullin 1
  • RING box protein 1
  • RING finger protein
  • ZYP protein
  • Synonym symbol(s) ROC1, MGC13357, MGC1481, RNF75, BA554C12.1
    EC.number 6.3.2.-
    DNA
    TYPE functioning gene
    STRUCTURE 21.28 kb     5 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    5 - 906 14 108 - 2007 17353931
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart   highly
    Digestivesalivary gland   highly
    Endocrineneuroendocrinepituitary  highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectivebone  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    Text expressed during embryogenesis
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a RING finger domain (C3H2C3) required for ubiquitin ligation
  • HOMOLOGY
    Homologene
    FAMILY
  • RING-box family
  • CATEGORY chaperone/stress , tumor suppressor
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus
    basic FUNCTION
  • having ubiquitin lyase activity
  • playing a unique role in the ubiquitination reaction by heterodimerizing with CUL1 to catalyze ubiquitin polymerization
  • also may be involved in the regulation of protein turn-over
  • involved in ubiquitination/degradation of cyclin D1 and E2F1, implicating RBX1 function in the regulation of G1/S transition of the cell cycle
  • might be responsible for regulating the turnover of RPS6KB1 and thereby would have an important function in controlling gene expression at the level of translation
  • physiological function of RBX1 is to ensure cell proliferation by preventing p27 accumulation during the early stage of embryonic development
  • key component of SCF E3 ligase and likely involves the regulation of many cellular processes
  • ensures proper cell proliferation at the early gastrulation stage of embryonic development
  • essential component of SCF E3 ubiquitin ligases for timely ubiquitination and degradation of many cellular proteins involved in growth regulation
  • plays a critical role in development
  • necessary for ubiquitin ligation activity of the multimeric cullin ring ubiquitin ligases (CRLs)
  • might play an important role in regulating the proliferation of gastric cancer cells and promoting the development of postoperative recurrence
  • CELLULAR PROCESS cell cycle
    protein, degradation
    protein, ubiquitin dependent proteolysis
    PHYSIOLOGICAL PROCESS
    text cell cycle control
    PATHWAY
    metabolism
    signaling
    a component
  • VHL complex, SCF (SKP1/CDC53-CUL1/F-box protein (SKP2) and APC (anaphase promoting) complex
  • RING component of SCF (Skp1, Cullins, F-box proteins) E3 ubiquitin ligases (NFE2L2 regulates CUL3-RBX1 by controlling regulation of expression and induction of CUL3-RBX1, and the induction of CUL3-RBX1 control NFE2Lf2 by increasing degradation)
  • FBXW5DDB1CUL4ARBX1 may function to regulate the homeostasis of TSC complexes instead of mediating a specific cellular growth condition
  • NFE2L2 serves as a substrate adaptor to link NFE2L2 to CUL3 and RBX1
  • CRL4 is a multisubunit protein complex, comprising cullin4A (CUL4A), RING H2 finger protein (RBX1), and DNA damage-binding protein 1 (DDB1)
  • KBTBD13 is able to form a complex with CUL3 and RBX1 to reconstitute a functional ubiquitin ligase capable of conjugating ubiquitin onto KBTBD13
  • complex between GLMN, RBX1, and a fragment of CUL1
  • essential component of the E3 ubiquitin ligase Skp1/Cullin/RBX1/F-box protein complex
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • cullins 1 and 2, phosphorylated NFKBIA
  • interacting with RPSKB1 (has the potential to regulate the turnover of both splicing forms of RPS6KB1)
  • mediates the neddylation of CUL1, which activates SCF E3 ligase activity by facilitating the ubiquitin transfer from E2 to substrates
  • KLHL20-CUL3-RBX1 E3 ligase complex promotes DAPK1 polyubiquitination, thereby inducing the proteasomal degradation of DAPK1, and controling interferon response
  • CUL3 is responsible for joining the ligase activity-bearing subunit RBX1 with a BTB-containing protein
  • GLMN binds directly to the RING domain of RBX1 and inhibits its E3 ubiquitin ligase activity
  • GLMN adopts a HEAT-like repeat fold that tightly binds the E2-interacting surface of RBX1, inhibiting CRL-mediated chain formation by the E2 CDC34
  • MAGEC2 binds directly to the RING domain protein RBX1, and participates in Skp1-Cullin1-F box protein (SCF) complex
  • cell & other
    REGULATION
    Other promoting nuclear accumulation of CUL1 and NEDD8
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    overexpressed in primary human tumor tissues and human cancer cell lines (
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    its silencing triggers multiple death and growth arrest pathways to effectively suppress tumor cell growth, suggesting that it may serve as a potential anticancer target
    cancerdigestivestomach
    novel therapy targeting RBX1 for gastric cancer
    ANIMAL & CELL MODELS
  • lethality of Rbx1Gt/Gt embryos is caused by proliferation failure at gastrulation as a result of p27 accumulation
  • indispensable role of Rbx1 in mouse oocyte meiotic maturation