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FLASH GENE
Symbol RAB35 contributors: mct/npt/pgu - updated : 28-04-2015
HGNC name RAB35, member RAS oncogene family
HGNC id 9774
Location 12q24.31      Physical location : 120.532.898 - 120.554.643
Synonym name
  • GTP-binding protein RAY
  • ras-related protein rab-1c (GTP-binding protein ray)
    • Synonym symbol(s) RAY, RAB1C, H-ray, Rab1c
    EC.number 3.6.5.1/ 3.6.5.2/3.6.5.3
    DNA
    TYPE functioning gene
    STRUCTURE 21.74 kb     6 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    6 - 2962 22.9 201 - 2008 18450757
    5 - 2835 - 152 - -
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Lymphoid/Immunethymus   highly
    Nervousbrain   highly
    Reproductivemale systemprostate  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • four conserved domains important for GTP binding
  • the C-terminus involved in membrane association
  • C-terminal polybasic cluster of the RAB35 hypervariable C-terminal domain (HVD) is essential for plasma membrane (PM) targeting, presumably because of the electrostatic interaction with negatively charged lipids on the PM
    • conjugated LipoP , Other
    HOMOLOGY
    interspecies homolog to Drosophila CG9575
    homolog to C.elegans 4R79.2
    Homologene
    FAMILY
  • small GTPase superfamily
  • Rab family
    • CATEGORY protooncogene , signaling
    SUBCELLULAR LOCALIZATION extracellular
        plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endosome
    intracellular,cytoplasm,cytosolic
    intracellular,nucleus
    text
  • accumulates at cell-cell contacts in a cadherin-dependent manner
  • RAB35 accumulates at ARF6-positive endosomes in response to nerve growth factor (NGF) stimulation and ACAP2 is recruited to the same compartment in a RAB35-dependent manner
  • in the nucleus, cytosol, and cell membrane
    • basic FUNCTION
  • GTPase activity
  • involved in vesicle transport
  • RAB35-regulated pathway plays an essential role during the terminal steps of cytokinesis by controlling septin and PIP2 subcellular distribution during cell division
  • TBC1D10C and RAB35 regulate a recycling pathway in T cells and contribute to immunological synapse (IS) formation, most likely by participating in TCR transport to the IS
  • playing a role in the regulation of cytoskeletal organization
  • play a role in recycling of the transferrin and other receptors and there is also evidence supporting a role for RAB35 in cytokinesis
  • might be involved in the formation of physiological extensions such as neurites
  • may be implicated in both cytokinesis, which involves remodelling of actin and tubulin cytoskeleton, and in endocytosis, which can involve interactions both with actin and with Rho-family GTPases
  • controls actin bundling by recruiting fascin as an effector protein
  • is a regulator of vesicle transport required specifically for phagocytosis
  • is the immune cell-specific regulator of vesicle transport within the actin-remodeling complex
  • localizes to the surface of oligodendroglia in a GTP-dependent manner, where it increases the density of vesicles, suggesting a function in docking or tethering
  • phosphocholination of RAB1A and RAB35 represents a mechanism by which bacterial FIC-domain-containing proteins can alter host-cell functions
  • is a critical upstream regulator of MICALL1 and ARF6, while both MICALL1 and ARF6 regulate RAB8A function
  • regulates endosomal membrane trafficking but also recruits effectors that modulate actin assembly and organization
  • RAB35 regulates cadherin-dependent adherens junctions (AJs) formation and myoblast fusion
  • key regulator of cargo recycling at endosomes, with an additional role in regulation of the actin cytoskeleton
  • RAB35 and its effector, ACAP2, a GTPase-activating protein that switches off ARF6 activity, negatively regulate oligodendrocyte morphological differentiation
  • regulates membrane trafficking from recycling endosomes to neurite tips during neurite outgrowth
  • molecular switch for membrane trafficking that regulates a variety of cellular events
  • small GTPase RAB35 functions as a molecular switch for membrane trafficking, specifically for endocytic recycling, by cycling between a GTP-bound active form and a GDP-bound inactive form
    • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • RAB35 forms a tripartite complex with MICALL1 and ACAP2 and recruits them to perinuclear ARF6-positive endosomes in response to nerve growth factor stimulation
    • INTERACTION
    DNA
    RNA
    small molecule nucleotide,
  • GTP
    • protein
  • RAB35 modulate TP53 transcriptional activity ofTP53RK
  • colocalizes with actin filaments and with CDC42, RAC1 and RHoA
  • OCRL is an effector of the RAB35 GTPase in cytokinesis abscission
  • RAB35 is a MICALL1-binding partner (over-expression of active RAB35 impairs the recruitment of MICALL1 to tubular recycling endosomes, whereas RAB35 depletion promotes enhanced MICALL1 localization to these structures)
  • likely RAB35 regulates actin-dependent phagosome formation by recruiting ACAP2, which might control actin remodeling and membrane traffic through ARF6
  • whereas DENND1A and DENND1B activate RAB35 for endosomal trafficking, DENND1C uniquely activates RAB35 for its role in actin regulation
  • interplay between RAB35 and ARF6 controls cargo recycling to coordinate cell adhesion and migration
  • regulates the formation of an EHD1-association site on ARF6-positive endosomes by integrating the functions of two distinct RAB35 effectors for successful neurite outgrowth
  • functions as a master RAB that determines the intracellular localization of MICALL1, which in turn functions as a scaffold for RAB8A, RAB13, and RAB36
  • CLIC4 is a regulator of RAB35 activity and serum- and LPA-dependent integrin trafficking
  • promotes neurite outgrowth of nerve growth factor-stimulated PC12 cells through interaction with ACAP2
  • RAB35 accumulates on bacteria-containing endosomes, and RAB35 directly binds and recruits CALCOCO2 to internalized bacteria
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS