Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

PRKCI

contributors: mct/npt/pgu - updated : 28-03-2013

HGNC name	protein kinase C, iota
HGNC id	9404

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	3q26.3      Physical location : 169.940.219 - 170.023.770
Synonym name	DNA segment, single copy, probe EST02087
	atypical protein kinase C-lambda/iota
Synonym symbol(s)	DXS1179E, PKCI, KPCI, MGC26534, nPKC-iota, APKC
EC.number	2.7.11.13

DNA

TYPE	functioning gene
STRUCTURE	83.55 kb     18 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

status

provisional

Map	cen - DXS174 - DXS118 - DXS96 - DXS173 - DXS442 - BTK - GLA - DXS178 - PRKCI - DXS265 - qter
Authors	(PMID: 7607695)

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_002740 18 - 4884 NP_002731 - 596 - 2008 17588663

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart       lowly Endocrine neuroendocrine pituitary     highly Nervous brain       highly Respiratory lung       highly Urinary bladder       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Epithelial secretory glandular endocrine   Muscular striatum skeletal   highly Nervous central

cell lineage

cell lines

fluid/secretion

at STAGE

physiological period

pregnancy

Text	placenta moderatly

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	tyrosine kinase, catalytic domain
	octicosapeptide repeat
	a zinc-dependent phorbol-ester
	DAG binding domain

HOMOLOGY

interspecies	homolog to C.elegans f09es.1
	homolog to prkcl

intraspecies

homolog to PRKCZ

Homologene

FAMILY	protein kinase superfamily
	AGC Ser/Thr protein kinase family
	PKC subfamily

CATEGORY

enzyme , signaling

SUBCELLULAR LOCALIZATION	extracellular
	plasma membrane
	intracellular
	intracellular,cytoplasm,organelle,membrane
	intracellular,cytoplasm,organelle,endosome
	intracellular,cytoplasm,cytosolic
	intracellular,nucleus

basic FUNCTION	involved in the secretory response to nutrients
	playing an essential role for protein transport in the early secretory pathway and required to promote RAB2-mediated vesicle budding at a vesicular tubular clusters subcompartment enriched in recycling cargo
	might play a compensatory role in TCR/CD28-induced signaling
	indirect co-regulator of HSF1 activity and the heat shock response
	regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus
	PARD6B and PRKCI control mitotic spindle orientation in polarized epithelia and, furthermore, that PRKCI coordinately regulates multiple processes to promote morphogenesis
	functions with CDC42 and PARD6B to control apical surface positioning
	promotes proper spindle orientation through the phosphorylation of downstream substrates, and promotes cell survival in a kinase-dependent manner
	expression of PRKCZ and PRKCI or both are dispensable for primitive and adult hematopoietic stem cell fate determination in steady-state and stress hematopoiesis
	phosphorylates ROCK1 and suppresses its junctional localization, thereby allowing cells to retain normally shaped apical domains
	phosphorylates NUMB to prevent its binding to CTNND1 and AP2A1, thereby attenuating CDH1 endocytosis to maintain apicobasal polarity

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

signal transduction

a component	PRKCI-PARD6A complex regulates the cytoplasmic localization of ECT2
	like CDC42, the PARD6B/PRKCI complex controls mitotic spindle orientation during cell division to correctly position the nascent apical surface in a growing cyst (
	FRMD6/PARD3-PRKCI-ROCK1 pathway that controls epithelial apical morphology
	PAR polarity complex of PARD3, PARD6A, and an atypical protein kinase C (PRKCI) regulate several aspects of neuronal migration

INTERACTION

DNA

RNA

small molecule

protein	interacting with RAB2
	PNMA1
	associates with CDK7
	interacting with PRKCZ and PRKCQ
	protects PARD6B from proteasomal degradation, mechanism ensuring that PARD6B is constitutively bound to and able to signal through PRKCI
	interacting with WWC1 (WWC1 suppresses apical exocytosis through inhibition of PRKCI activity in epithelial cells)
	interacting with CDC42 (CDC42 controls vascular network assembly through PRKCI during embryonic vasculogenesis)
	RAC1 exchange factor TIAM1 participates in polarized cell migration with the PAR complex of PARD3, PARD6A, and PRKCI
	PRKCI, interacts with TGFB1 receptors through PARD6A and these proteins localize to the leading edge of migrating cells
	PARD6A-PRKCI recruitment to the premature apical membrane appears to be required for definition of apical identity of epithelial cells
	CLASP2 directly interacted with PARD3 and was phosphorylated by PRKCI

cell & other

REGULATION

activated by	lipids and Ca2+
	diacylglycerol which turn phosphorylates in a range of cellular proteins

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in esopageal squamous cell carcinoma with presence of lymph node metastasis

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed immunology inflammatory   may constitute potential therapeutic targets for inflammatory joint diseases involving increased collagenase expression

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in esopageal squamous cell carcinoma with presence of lymph node metastasis

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed immunology inflammatory   may constitute potential therapeutic targets for inflammatory joint diseases involving increased collagenase expression

ANIMAL & CELL MODELS