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FLASH GENE
Symbol POLRMT contributors: mct - updated : 28-03-2014
HGNC name polymerase (RNA) mitochondrial (DNA directed)
HGNC id 9200
Location 19p13.3      Physical location : 617.222 - 633.568
Synonym name mitochondrial DNA-directed RNA polymerase
Synonym symbol(s) APOLMT, MTRPOL, RPOM, MTRNAP, h-mtRPOL
EC.number 2.7.7.6
DNA
TYPE functioning gene
STRUCTURE 16.34 kb     21 Exon(s)
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked N status provisional
Map pter - D17S791 - D17S797 - D17S1795 - ITGA3 - D17S788 - D17S790 - HLF HLF - D17S1607 - MPO - D17S792 - POLRMT - D17S808 - D17S794 - cen
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
21 - 3800 - 1230 - 1997 9097968
EXPRESSION
Type widely
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Endocrineadrenal gland   highly
Nervousnervecranial nerve  highly
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
HOMOLOGY
Homologene
FAMILY phage and mitochondrial RNA polymerase family
CATEGORY enzyme , DNA associated
SUBCELLULAR LOCALIZATION     intracellular
intracellular,cytoplasm,organelle,mitochondria,matrix
basic FUNCTION
  • DNA-dependent RNA polymerase catalyzing the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
  • may be part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression
  • primase required for initiation of DNA synthesis from the light-strand origin of DNA replication
  • can function as an origin-specific primase in mammalian mitochondria
  • serves as both the transcriptase for expression and the primase for replication of mitochondrial DNA
  • requires the factors TFAM and TFB2M for binding and melting promoter DNA
  • early phage-like POLRMT lost functions in promoter binding and melting, which were provided by initiation factors in trans during evolution, to enable mitochondrial gene regulation and the adaptation of mitochondrial function to changes in the environment
  • organization of TFAM, POLRMT and TFB2M around thelight-strand promoter (LSP), represent the first structural characterization of the entire mitochondrial transcriptional initiation complex
  • TFAM first recruits POLRMT to the promoter, followed by TFB2M binding and induction of promoter melting
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • POLRMT/TFB2M heterodimer is unable to interact with promoter elements and initiate even abortive transcription in the absence of TFAM
  • TFB2M along with two other proteins, POLRMT and TFAM, are key components of the core mitochondrial transcription apparatus
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with TFB2M (heterodimer is unable to interact with promoter elements and initiate even abortive transcription in the absence of TFAM)
  • interacting with MRPL12
  • TEFM interacts with the catalytic region of POLRMT
  • MRPL12 interacts with TFB2M and POLRMT (MRPL12 and TFB2M bind POLRMT in distinct protein complexes)
  • POLRMT needs both TFB2M and TFAM to melt the promoter
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS