Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

POLRMT

contributors: mct - updated : 28-03-2014

HGNC name	polymerase (RNA) mitochondrial (DNA directed)
HGNC id	9200

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	19p13.3      Physical location : 617.222 - 633.568
Synonym name	mitochondrial DNA-directed RNA polymerase
Synonym symbol(s)	APOLMT, MTRPOL, RPOM, MTRNAP, h-mtRPOL
EC.number	2.7.7.6

DNA

TYPE	functioning gene
STRUCTURE	16.34 kb     21 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

Map	pter - D17S791 - D17S797 - D17S1795 - ITGA3 - D17S788 - D17S790 - HLF HLF - D17S1607 - MPO - D17S792 - POLRMT - D17S808 - D17S794 - cen

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_005035 21 - 3800 NP_005026 - 1230 - 1997 9097968

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Endocrine adrenal gland       highly Nervous nerve cranial nerve     highly

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

HOMOLOGY

Homologene

FAMILY

phage and mitochondrial RNA polymerase family

CATEGORY

enzyme , DNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,organelle,mitochondria,matrix

basic FUNCTION	DNA-dependent RNA polymerase catalyzing the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates
	may be part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression
	primase required for initiation of DNA synthesis from the light-strand origin of DNA replication
	can function as an origin-specific primase in mammalian mitochondria
	serves as both the transcriptase for expression and the primase for replication of mitochondrial DNA
	requires the factors TFAM and TFB2M for binding and melting promoter DNA
	early phage-like POLRMT lost functions in promoter binding and melting, which were provided by initiation factors in trans during evolution, to enable mitochondrial gene regulation and the adaptation of mitochondrial function to changes in the environment
	organization of TFAM, POLRMT and TFB2M around thelight-strand promoter (LSP), represent the first structural characterization of the entire mitochondrial transcriptional initiation complex
	TFAM first recruits POLRMT to the promoter, followed by TFB2M binding and induction of promoter melting

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component	POLRMT/TFB2M heterodimer is unable to interact with promoter elements and initiate even abortive transcription in the absence of TFAM
	TFB2M along with two other proteins, POLRMT and TFAM, are key components of the core mitochondrial transcription apparatus

INTERACTION

DNA

RNA

small molecule

protein	interacting with TFB2M (heterodimer is unable to interact with promoter elements and initiate even abortive transcription in the absence of TFAM)
	interacting with MRPL12
	TEFM interacts with the catalytic region of POLRMT
	MRPL12 interacts with TFB2M and POLRMT (MRPL12 and TFB2M bind POLRMT in distinct protein complexes)
	POLRMT needs both TFB2M and TFAM to melt the promoter

cell & other

REGULATION

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS