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FLASH GENE
Symbol OTUB1 contributors: mct - updated : 13-01-2020
HGNC name OTU domain, ubiquitin aldehyde binding 1
HGNC id 23077
Location 11q13.1      Physical location : 63.753.324 - 63.765.892
Synonym name
  • otubain
  • ubiquitin-specific protease otubain 1
  • OTU-domain Ubal-binding protein
  • otubain-1
    • Synonym symbol(s) OTB1, OTU1, FLJ20113, FLJ40710, MGC4584, HSPC263, MGC111158
    EC.number 3.4.19.12
    DNA
    TYPE functioning gene
    STRUCTURE 12.57 kb     7 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    7 - 2310 - 271 - 2009 18954305
    EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestivemouthtongue   
    Nervousbrain    
    Visualeye    
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Blood/Hematopoieticleukocyte
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N-terminal helix of OTUB1 is positioned to interfere with UBE2V1 binding to UBE2N, as well as with attack on the thiolester by an acceptor ubiquitin, thereby inhibiting K63Ub synthesis
  • one OTU domain with an active cysteine protease site
  • UB-interaction motifs (UIMs) and UB-associated (UBA) domains
  • putative nuclear localization signals
  • a consensus LxxLL motif
    • HOMOLOGY
    Homologene
    FAMILY
  • ovarian tumor (OTU) protein superfamily
  • peptidase C65 family
    • CATEGORY enzyme , regulatory
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,mitochondria,inner
    intracellular,cytoplasm,organelle,membrane
    basic FUNCTION
  • playing an important regulatory role at the level of protein turnover by preventing degradation
  • deubiquitinating enzyme that is implicated in mediating lymphocyte antigen responsiveness
  • regulates the availability and functional activity of ESR1 in Ishikawa cells by affecting the transcription of the ESR1 and by stabilizing the ESR1 protein in the chromatin
  • suppresses RNF168-dependent poly-ubiquitination independently of its catalytic activity
  • with OTUB2, are negative regulators of virus-triggered type I IFN induction
  • negatively regulate virus-triggered type I IFN induction and cellular antiviral response by deubiquitinating TRAF3 and TRAF6
  • inhibitor of DNA double-strand breaks-induced chromatin ubiquitination
  • deubiquitylase, inhibiting the DNA damage response independently of its isopeptidase activity
  • regulates NFKB and MAPK signalling pathways and TNF-dependent cell death by modulating BIRC2 stability
  • OTUB1 plays an essential role in controlling the stability of SLC7A11 and the CD44-mediated effects on ferroptosis in human cancers
  • both OTUB1 and OTUB2 display a catalytic triad characteristic of proteases but differ in their configuration and protonation states
  • OTUB1, OTUB2 are potential therapeutic targets against aging-related disorders
    • CELLULAR PROCESS protein, degradation
    protein, ubiquitin dependent proteolysis
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacts with CDC48, a regulator of the ER-associated degradation pathway
  • ESR1-interacting protein capable of deubiquitinating ESR1 in cells
  • OTUB1 and -2 interacted with TRAF3 and TRAF6, two E3 ubiquitin ligases required for virus-triggered IRF3 and NF-kappaB activation, respectively
  • counteracts RNF8/RNF168-dependent ubiquitin chain formation at break sites
  • binding to and inhibiting UBE2N, the cognate E2 enzyme for RNF168 and pharmacological targeting of the OTUB1-UBE2N interaction might enhance the DNA damage response
  • OTUB1 interacted with DCN (the overexpression of OTUB1 enhanced the ubiquitination and degradation of DCN in mesangial cells (MC)
  • free ubiquitin associates with the canonical distal ubiquitin-binding site on OTUB1 to promote formation of the inhibited E2 complex
  • inhibits UBE2N and other E2 enzymes
  • OTUB1 inhibits Lys-63-linked ubiquitination by binding to a ubiquitin-conjugating enzyme UBE2N
  • is a Lys48-specific deubiquitinating enzyme that forms a complex with E2 ubiquitin (Ub)-conjugating enzymes including UBE2N and UBE2D1
  • UBE2D1 preferentially binds to the monoubiquitinated OTUB1 via UB interaction with its backside donor Ub-interacting surface
  • NMRAL1 interacted with TRAF3 and inhibited its ubiquitination by promoting the recruitment of OTUB1 to TRAF3
  • OTUB1 directly interacted with and stabilized SLC7A11
    • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --over  
    in the mesangial area of glomeruli in some immunocomplex mediated nephritides such as IgA nephropathy, acute diffuse proliferative glomerulonephritis
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    SystemTypeDisorderPubmed
    cancer  
    potential target in cancer therapy
    ANIMAL & CELL MODELS