N-terminal, Rho-GEF, and the non-modular C-terminal domain that lacks the kinase-like domains, localizes at the level of the M-band
modular protein of striated muscles, binds to small ankyrin 1 (sAnk1, Ank 1.5), an integral protein of the sarcoplasmic reticulum, through two distinct C-terminal sequences (PMID: 20949908)
106
-
24030
900
7968
not restricted to striated muscles, but are abundantly expressed in several tissues and organs including brain, skin, kidney, liver, spleen, and lung
2007
17382936
Obscurin B, or giant kinase isoform
N-terminal, Rho-GEF, and the C-terminal kinase-like domains, localizes at the level of the A/I junction
tandem structural and signaling motifs, including 2 serine/threonine kinase domains, SK1 and SK2, present at the C- terminus of giant obscurin-B
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-
26925
-
8923
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-
EXPRESSION
Type
restricted
expressed in
(based on citations)
organ(s)
System
Organ level 1
Organ level 2
Organ level 3
Organ level 4
Level
Pubmed
Species
Stage
Rna symbol
Digestive
liver
Homo sapiens
Endocrine
thyroid
Lymphoid/Immune
spleen
Homo sapiens
Nervous
brain
Homo sapiens
Respiratory
lung
Homo sapiens
Skin/Tegument
skin
Homo sapiens
Urinary
kidney
Homo sapiens
tissue
System
Tissue
Tissue level 1
Tissue level 2
Level
Pubmed
Species
Stage
Rna symbol
Muscular
striatum
cardiac
Homo sapiens
Muscular
striatum
skeletal
Homo sapiens
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
two fibronectin type III domain
>68 immunoglobulin domains
pleckstrin homology domain
two serine/threonine kinase domains
one calcium/calmodulin binding domain
several interacting modules, one of which mediates binding to muscle-specific ankyrins
a C terminal Rho GEF domain, with two distinct binding sites to mediate binding with ANK1I5 and ANK1I9, rhoGEF domain and tandem pleckstrin homology domain, are consistent with a role in rho signaling in muscle
DBL family of guanine nucleotide exchanges factor and titin family of myosin light chain kinases (MLCK)
CATEGORY
structural protein
SUBCELLULAR LOCALIZATION
intracellular
intracellular,cytoplasm,cytoskeleton
text
sarcomere
basic FUNCTION
giant muscle protein involved in Ca2+/calmodulin and G protein-couples signal transduction in the sarcomere
having important functions in cardiac and skeletal muscle physiology
C-terminal region interacts with sarcomeric myosin and may play a critical role in its ability to assemble into A bands in striated muscle
play several distinct roles in organizing and stabilizing the contractile apparatus and nearby structures
playing a role in mediating the subcellular localization of small ANK1 isoforms in striated muscle cells
multidomain protein composed of adhesion and signaling domains that plays key roles in the organization of contractile and membrane structures in striated muscles
modular protein of striated muscle that concentrates around the M-bands and Z-disks of each sarcomere, where it is well positioned to sense contractile activity
its rhoGEF domain signals at least in part by inducing RHOA expression and activation, and altering the expression of downstream kinases in skeletal muscle
OBSCN and KCTD6 regulate cullin-dependent small ankyrin-1 (ANK1) protein turnover
play essential roles in myofibrillogenesis, cytoskeletal organization, and Ca(2+) homeostasis
may play common and unique roles, respectively, in the regulation and maintenance of cell homeostasis in various tissues and organs throughout the body
giant cytoskeletal protein with structural and regulatory roles
CELLULAR PROCESS
PHYSIOLOGICAL PROCESS
PATHWAY
metabolism
signaling
signal transduction
signaling pathway in skeletal muscle that involves obscurin and the Rho GTPase RHOQ and implicate this pathway in new sarcomere formation
a component
INTERACTION
DNA
RNA
small molecule
protein
N terminal Z-disk of titin
ligand for small ankyrin 1 in skeletal muscle (obscurin and small ANK1 isoforms may form stable interactions that may be relevant to connect the sarcoplasmic reticulum and the contractile apparatus in skeletal muscle cells)
obscurin/ANK2 interaction is critical for recruitment of PPP2A to the cardiac M-line
specific activator of RHOQ but not the Rho GTPases RAC1 and CDC42
a new ligand of obscurin at the M-band is MYBPC1, suggesting that their interaction contributes to the assembly of M- and A-bands
by targeting ANK2 at the M band, contributes to the organization of subsarcolemma microtubules, localization of dystrophin at costameres, and maintenance of sarcolemmal integrity
