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FLASH GENE
Symbol NUCB1 contributors: mct - updated : 18-05-2012
HGNC name nucleobindin 1
HGNC id 8043
Location 19q13.32      Physical location : 49.403.306 - 49.426.539
Synonym name
  • calnuc
  • Synonym symbol(s) DT1P1A2, NUC, FLJ40471, CALNUC, DKFZp686A15286
    DNA
    TYPE functioning gene
    SPECIAL FEATURE arranged in tandem, head to head
    STRUCTURE 23.23 kb     12 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    text structure TATA less
    MAPPING cloned Y linked N status confirmed
    RNA
    TRANSCRIPTS type messenger
    text 1
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    13 - 2585 - 461 - 2009 1949705
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Endocrineadrenal gland   highly
    Lymphoid/Immunespleen   highly
     thymus   highly
    Nervousbrain    
    Reproductivemale systemprostate  highly
    Urinarykidney    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal  
    cell lineage
    cell lines
    fluid/secretion secreted
    at STAGE
    physiological period fetal, pregnancy
    Text placenta, heart, lung, liver, kidney, brain
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N-terminal fragment (first 220 AAs) comprised the putative DNA binding region
  • three evolutionarily conserved N-linked glycosylation sites within its carboxyl luminal domaina signal peptide
  • five or more CAG repeats
  • NUCB1 and NUCB2, two highly homologous calcium-binding proteins, share a common motif with CCDC88A for Galpha(i) binding and activation
  • a Ca(2+)-binding domain which is unstructured in the absence of Ca2+ and folds upon Ca2+ addition
  • Ser-378 of GXSXG motif, Asp-328 of DTG motif, and His-339 form the "catalytic triad," locating the enzyme active site in the C-terminal region , and C-terminal fragment contained the two EF-hand motifs, G protein-binding site, and the leucine zipper domain
  • HOMOLOGY
    interspecies ortholog to murine Nucb
    homolog to rattus Nucb
    homolog to Drosophila NUCB1
    homolog to C.elegans F44A6.1
    Homologene
    FAMILY nucleobindin family
    CATEGORY secretory
    SUBCELLULAR LOCALIZATION extracellular
        intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,endoplasmic reticulum
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytoskeleton,microtubule
    text
  • in the cis-Golgi, tightly associated with the luminal side of the membrane
  • calcium (Ca2+) binding protein found in both Golgi and cytoplasm
  • Ca(++)-binding protein found in the cytoplasm where it binds different Galpha subunits, in the Golgi lumen where it constitutes a major Ca(++) storage pool, and outside the cell
  • basic FUNCTION
  • playing a role in calcium homeostasis
  • playing a role as a housekeeping gene
  • major calcium-binding protein of the golgi that may have a role in calcium homeostasis
  • Golgi-localized negative feedback regulator in the ATF6-mediated branch of the unfolded protein response (suppresses the S1P-mediated cleavage activation of ATF6 in the Golgi apparatus)
  • participates in Ca2+ storage in the Golgi, as well as in other biological processes that involve DNA-binding and protein-protein interactions
  • NUCB1 and NUCB2 are multifunctional proteins that interact with Ca(2+), nucleic acids and various regulatory proteins in different signaling pathways
  • plays a role in receptor trafficking
  • Golgi-localized soluble protein with a signal peptide and multiple functional domains, and negative regulator of the unfolded protein response that activates various endoplasmic reticulum (ER)-originating signaling pathways
  • multidomain calcium-binding protein, that might interact with heterotrimeric G protein alpha subunits
  • cytoplasmic NUCB1 might function to regulate heterotrimeric G protein trafficking and G protein-coupled receptor-mediated signal transduction pathways
  • prevent islet amyloid polypeptide (hIAPP) fibril formation and disaggregate preexisting hIAPP fibrils, and may offer a new approach to inhibiting amyloid formation and disaggregating amyloid fibrils
  • is a serine protease with its active site catalytic triad present in the C-terminal domain
  • acting as a Ca2+-binding protein in Zn2+-bound form and as a protease in Zn2+-free formas
  • EF-hand containing Ca2+ binding serine protease
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA binding
    RNA
    small molecule metal binding,
    calcium Ca2+
    protein
  • binding to GNAI2 and GNAI3
  • its functions could be modulated by caspase-mediated cleavage in apoptosis
  • interaction between NUCB1 and LRP9 (affects LRP9 endosomal sorting)
  • NUCB1 prevent IAPP fibril formation and disaggregate preexisting IAPP fibrils
  • cell & other
    REGULATION
    Other both Zn2+ and G proteins function as two different keys that lock and regulate the protease activity of NUCB1
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
  • might provide a novel non-invasive approach to enhance antibody detection for colon cancer diagnosis
  • Therapy target
    ANIMAL & CELL MODELS
    secreted protein promoting production of DNA-specific antibodies in lupus-prone MRL/lpr mouse