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FLASH GENE

Symbol

NT5E

contributors: mct/shn - updated : 12-06-2013

HGNC name	5'-nucleotidase, ecto (CD73)
HGNC id	8021

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	6q14.3      Physical location : 86.159.301 - 86.205.496
Synonym name	antigen CD73,identified by antibodies 1E9.8,7G2.2.11,AD2
	5' nucleotidase (CD73)
	5' nucleotidase, ecto precursor
	5-prime-ribonucleotide phosphohydrolase
	Ecto-5-prime-nucleotidase
	nucleotidase ecto-5-prime
Synonym symbol(s)	CD73, NT5, P5N, E5NT, NT, NTE, eN, eNT, RP11-321N4.1
EC.number	3.1.3.5

DNA

TYPE	functioning gene
STRUCTURE	45.00 kb     9 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

confirmed

Map	cen - D6S1601 - D6S1652 - CD73 - D5S1482 - D6S1004 - qter

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_002526 9 - 4087 NP_002517 - 574 - -

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular heart         Endocrine thyroid       highly Respiratory respiratory tract trachea     highly Skin/Tegument skin       highly Urinary kidney         Visual eye

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Blood / Hematopoietic

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic leukocyte

cell lineage

cell lines

fluid/secretion

at STAGE

physiological period

pregnancy

Text	placenta, retina

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

conjugated

GlycoP

mono polymer

homomer , dimer

HOMOLOGY

interspecies	ortholog to Nt5e, Mus musculus
	ortholog to nt5e, Danio rerio
	ortholog to Nt5e, Rattus norvegicus
	ortholog to NT5E, Pan troglodytes

Homologene

FAMILY

5'-nucleotidase family

CATEGORY

enzyme

SUBCELLULAR LOCALIZATION	plasma membrane
text	bounds by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane
	expressed on normal and neoplastic glial plasma membranes (Ludwig et al, 1999)

basic FUNCTION	cell surface enzyme of the purine catabolic pathway that catalyzes the breakdown of AMP to adenosine
	hydrolyzing extracellular nucleotides into membrane permeable nucleosides
	may facilitate the adhesion, migration and invasion of human breast cancer cells through its enzyme activity of generating adenosine
	required for the efficient entry of lymphocytes into the CNS during experimental autoimmune encephalomyelitis development
	novel and specific receptor for tenascin C and the interaction between these proteins may influence cell adhesion and migration and also lead to decreased generation of local adenosine (Sadej 2008)
	a signalling pathway metabolite in the immune response of lymphocytes
	a cell surface marker of cone/rod common precursors and mature rod cells genetically localized between Nrl and Crx (Koso 2009)
	extracellular adenosine, generated in tandem by ecto-enzymes ENTPD1 and NT5E, promotes dermal fibrogenesis
	ENTPD1, NT5E are two ectonucleotidases that cooperate in the generation of extracellular adenosine through ATP hydrolysis, thus tilting the balance towards immunosuppressive microenvironments

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

text	catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides

PATHWAY

metabolism

purine/pyrimidine

signaling

nucleotide

a component	glycoprotein constituent of membrane (Pi linked)
	component of multiresistance machinery, the transcription of which is activated under selective pressure of the immune system (Ludwig et al, 1999)

INTERACTION

DNA

RNA

small molecule	cofactor,
	zinc

protein

intracellular filamentous actin and the extracellular matrix proteins laminin and fibronectin (Ludwig et al, 1999)

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral     --over   in melanoma with with a highly invasive phenotype

Susceptibility

Variant & Polymorphism

Candidate gene
Marker
Therapy target
	System Type Disorder Pubmed dermatology skin   biochemical or biological inhibitors of ENTPD1 and/or NT5E may hold promise in the treatment of dermal fibrosis in diseases such as scleroderma

ANIMAL & CELL MODELS

ecto-5'-nucleotidase/CD73-deficient mice have a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow (Castrop et al, 2004)