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FLASH GENE
Symbol NONO contributors: mct - updated : 31-10-2015
HGNC name non-POU domain containing, octamer-binding
HGNC id 7871
Corresponding disease
MRXS34 mental retardation, syndromic 34
Location Xq13.1      Physical location : 70.503.041 - 70.521.016
Synonym name
  • nuclear RNA binding protein (p54 nrb)
  • NonO protein
  • non-POU domain-containing octamer (ATGCAAAT) binding
  • protein
  • DNA-binding p52/p100 complex, 52 kDa subunit
  • Synonym symbol(s) P54, NRB54, NRB1, NMTS5, P54NRB
    DNA
    TYPE functioning gene
    STRUCTURE 17.98 kb     13 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    text structure start codon in exon 3
    MAPPING cloned Y linked N status confirmed
    Map cen - DXS106 - DXS453 - FOXO4 - IL2RG - GJB1 - DXS7119 - NONO - ZMYM3 - TAF1 - DXS559 - qter
    Authors Haberhausen (95),Villard (95),Consensus (95)
    Physical map
    OBDPF Xq13.1 osteoblast differentiation promoting factor DLG3 Xq13.1 discs, large (Drosophila) homolog 3 (neuroendocrine-dlg) TEX11 Xq12-q13.1 testis expressed sequence 11 SLC7A3 Xq12 solute carrier family 7 (cationic amino acid transporter, y+ system), member 3 LOC392488 X similar to Nuclear transport factor 2 (NTF-2) (Placental protein 15) (PP15) SNX12 Xq12-q13.1 sorting nexin 12 MLLT7 Xq13.1 myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 7 LOC158830 Xq13.1 similar to Ab2-183 IL2RG Xq12-q13.1 interleukin 2 receptor, gamma (severe combined immunodeficiency) TNRC11 Xq13 trinucleotide repeat containing 11 (THR-associated protein, 230kDa subunit) NLGN3 Xq13.1 neuroligin 3 GJB1 Xq13.1 gap junction protein, beta 1, 32kDa (connexin 32, Charcot-Marie-Tooth neuropathy, X-linked) ZNF261 Xq13.1 zinc finger protein 261 NONO Xq13.1 non-POU domain containing, octamer-binding ITGB1BP2 Xq12.1-q13 integrin beta 1 binding protein (melusin) 2 LOC392489 X similar to ras homolog gene family, member G (rho G); RhoG TAF1 Xq13.1 TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa ING2 Xq12 inhibitor of growth family, member 2 OGT Xq13 O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) ACRC Xq13.1 acidic repeat containing CXCR3 Xq13 chemokine (C-X-C motif) receptor 3 LOC158825 Xq13.1 hypothetical LOC158825 LOC389868 X LOC389868 LOC389869 X LOC389869 LOC389870 X LOC389870 LOC389871 X LOC389871 LOC286539 Xq13.1 similar to 40S ribosomal protein S26
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    13 - 3228 - 471 - 2008 18952062
    11 - 3057 - 471 - 2008 18952062
    10 - 2894 - 382 - 2008 18952062
    12 - 3114 - 471 - 2008 18952062
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
    Endocrinepancreas    
    Nervousbrainmidbrainhypothalamussupraoptic nuclei  Mus musculus
    Reproductivemale systemtestis  highly Homo sapiens
    Urinarykidney    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal  
    cells
    SystemCellPubmedSpeciesStageRna symbol
    ReproductiveSertoli cell Homo sapiens
    cell lineage
    cell lines a number of breast tumor cell lines
    fluid/secretion
    at STAGE
    physiological period pregnancy
    Text placenta
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • two RNA-binding (RNA recognition motif [RRM]) domains
  • unique regions rich in glutamine, histidine, arginine and glutamine acid
  • a NOPS domain, characteristic of DBHS proteins, specifically binding to RRM2 of the partner DBHS protein
  • a protein phosphatase 1 (PP1) consensus binding RVXF motifs, playing an important role in controlling the multifunctional properties of NONO and SFPQ in the regulation of gene transcription
  • C-terminal coiled-coil motif essential for paraspeckle localization
  • mono polymer heteromer , dimer , tetramer
    HOMOLOGY
    interspecies homolog to Drosophila NONA/BJ6
    homolog to murine Nono
    homolog to C.elegans f25b5.7
    intraspecies homolog to highly in splicing factor PSF
    Homologene
    FAMILY
  • DBHS (Drosophila melanogaster behavior, human splicing) family
  • CATEGORY RNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,nucleoplasm,nuclear bodies,nuclear speckles
    intracellular,nucleus,chromatin/chromosome
    intracellular,nucleus,nucleolus
    text
  • predominately nuclear
  • in interphase cells, localizes to the nucleoplasm and concentrates with protein partners in the paraspeckles via an interaction with the non-coding RNA NEAT1
  • basic FUNCTION
  • functioning as a coactivator of androgen receptors that potentiates transcription and possibly splicing
  • with PSPC1 and SFPQ, may cooperatively regulate androgen receptor-mediated gene transcription
  • plays an important role in the regulation of Sox9 function and the formation of paraspeckle bodies during chondrogenesis
  • acting as a bridge between the CREB/TORC complex and RNA polymerase II (Amelio 2007)
  • having a functions in recruitment of XRN2 to facilitate pre-mRNA 3' processing and transcription termination (Kaneko 2007)
  • core paraspeckle proteins
  • SFPQ/NONO heterodimer was previously found to enhance DNA strand break rejoining
  • critical to the control of gene expression through the nuclear retention of RNA containing double-stranded RNA regions that have been subject to adenosine-to-inosine editing
  • involved in many nuclear processes including transcription, RNA processing, and retention of hyperedited RNAs
  • involvement of NONO and SFPQ in DNA double-strand break repair (DSB) and radioresistance
  • serve as transcriptional cofactor for correct circadian clock function, where it regulate the circadian clock via interaction with PER proteins
  • important role of NONO, PSPC1, SFPQ within the circadian oscillator
  • overlapping or redundant roles of NONO, PSPC1, SFPQ as transcriptional cofactors at circadian clock-regulated genes
  • regulates synaptic transcription and gephyrin scaffold structure
  • may likely contribute to the regulation of RNA metabolism underlying local regulation of dendritic spine morphology and has a major and unsuspected role in regulating synaptic morphology at a cellular level
  • CELLULAR PROCESS nucleotide, RNA splicing
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • heterotetramer of two 52kDa and two 100kDa subunits
  • SFPQ/NONO heterodimer
  • PSPC1/NONO heterodimer has a right-handed antiparallel coiled-coil that positions two of four RNA recognition motif domains in an unprecedented arrangement
  • INTERACTION
    DNA
    RNA
  • interaction with RNA could be selectively modulated by phosphorylation during mitosis
  • small molecule
    protein
  • interacting with SOCS3 (SOCS3 acts as a suppressor by regulating the action of NONO)
  • interacting with FAM123B
  • interacting with PARK7 and SFPQ (binding partner in dopaminergic neuronal cells)
  • HNRNPM interacts with SFPQ and NONO and co-localizes within defined nuclear structures
  • SFPQ interacts directly with RAD51 and deficiency of both proteins confers a severe loss of cell viability, indicating a synthetic lethal relationship
  • GJA1 is a downstream target molecule of NONO
  • partner of circadian PERIOD (PER) proteins
  • bound to the CDKN2A cycle checkpoint gene and potentiated its circadian activation in a PER protein-dependent fashion
  • is essential for glucocorticoids-mediated expression of occludin, CLDN5, and barrier induction, and the NONO/SFPQ heterodimer may contribute to normal blood-retinal barrier (BRB) induction
  • although NONO and SFPQ are nuclear paraspeckle components, these proteins formed cytoplasmic granules with RBPMS in the neurites
  • cooperative function of HNRNPM and NONO as regulators of internal ribosome entry site (IRES)-dependent translation, indicating the involvement of a promoter-dependent mechanism
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s) MRXS34
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral        
    loss of expression maybe related to events involved in hormone insensitivity, tumor differentiation, and unregulated tumor cell growth and metastases
    tumoral     --over  
    highly expressed in malignant melanoma
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • fibroblasts from Nono-deficient mice showed approximately normal circadian cycles, they displayed elevated cell doubling and lower cellular senescence
  • Nono-deficient mice have cognitive and affective deficits