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FLASH GENE
Symbol NEIL2 contributors: mct/npt - updated : 18-12-2014
HGNC name nei like 2 (E. coli)
HGNC id 18956
Location 8p23.1      Physical location : 11.627.171 - 11.644.854
Synonym name
  • DNA-(apurinic or apyrimidinic site) lyase Neil2
  • DNA glycosylase/AP lyase Neil2
  • Nei-like 2
  • Synonym symbol(s) NEH2, FLJ31644, MGC2832, MGC4505
    EC.number 4.2.99.18, 3.2.2.-,
    DNA
    TYPE functioning gene
    STRUCTURE 17.68 kb     5 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence
    text structure single nucleotide polymorphisms 5 prime upstream the coding region of the NEIL2 gene influence gene transcription levels and alter levels of genetic damage (Kinslow 2008)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    5 - 2202 - 332 - 2004 15339932
    4 - 2062 - 271 - 2004 15339932
    5 - 2016 - 216 - 2004 15339932
    5 - 2746 - 332 - 2004 15339932
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Endocrineadrenal gland   highly
    Reproductivefemale systemuteruscervix highly
     male systemtestis  highly
    Urinarybladder   highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal  
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a N-terminal Pro as the active site
  • a potential helix-2-turn-helix motif
  • zinc finger motifs, essential for its structural integrity and enzyme activity
  • Cys-X2-His-X16-Cys-X2-Cys (CHCC) motif present near the C terminus is distinct from the zinc finger motifs of NEI/Fpg
  • HOMOLOGY
    interspecies ortholog to E.coli MutM/Nei
    Homologene
    FAMILY
  • E.coli MutM/Nei family
  • DNA glycosylases family
  • FPG family
  • CATEGORY enzyme , DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,mitochondria
    intracellular,cytoplasm,cytoskeleton,microtubule
    intracellular,nucleus
    text
  • predominantly localized in the nucleus despite the absence of a nuclear localization signal
  • in spindle microtubules
  • presence of NEIL2 and PNKP in mitochondria
  • basic FUNCTION
  • DNA glycosylase/AP lyase activity, involved in repair of lesions in DNA generated during transcription and/or replication, mainly by removing oxidative products of cytosine
  • may have a novel regulatory role in NEIL2-mediated repair under oxidative stress (Das 2007)
  • recognizes an apurinic/apyrimidinic site exclusively
  • able to excise base lesions from single-stranded DNA regions suggesting their preferential involvement in repair during replication and/or transcription (Dou 2008)
  • excises oxidized DNA lesions from bubble or single-stranded structures, suggesting its involvement in transcription-coupled DNA repair (Kinslow 2008)
  • critical role of NEIL2 and PNKP in maintenance of the mammalian mitochondrial genome
  • oxidized base-specific DNA glycosylase, that is unique in preferentially repairing oxidative damage in transcribed genes
  • NEIL1-mediated prereplicative repair of oxidized bases in the replicating strand, with NEIL2 providing a backup function
  • biological role for ERCC6 and NEIL2 in transcription associated base excision repair
  • CELLULAR PROCESS nucleotide, repair, base excision repair
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA binding
    RNA
    small molecule
    protein
  • Y box-binding (YBX1) protein is a stably interacting partner of NEIL2 (not only interacts physically with NEIL2, but it also cooperates functionally by stimulating its base excision activity by 7-fold)(Das 2007)
  • co-association of NEIL2 and PNKP with POLG on the mitochondrial genome
  • ERCC6, and NEIL2 were found to physically interact independently of DNA
  • NEIL1 and NEIL2 DNA glycosylases coordinate abasic-site processing during TET-TDG DNA demethylation, and NEIL1 and NEIL2 cooperate with TDG during base excision
  • cell & other
    REGULATION
    inhibited by significantly inhibited by
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional   deletion    
    in congenital heart disease
    Susceptibility
  • to familial colorectal cancer
  • to lung cancer development
  • Variant & Polymorphism other
  • missense variants increasing the risk of colorectal cancer
  • decreased DNA repair capacity of the R257L variant can induce mutations that lead to lung cancer development
  • Candidate gene
  • polymorphisms of the NEIL2 gene may be markers for risk and progression of squamous cell carcinoma of the oral cavity and oropharynx, particularly in patients with oropharyngeal cancer (Zhai 2008)
  • Marker
    Therapy target
    ANIMAL & CELL MODELS