Selected-GenAtlas references SOURCE GeneCards NCBI Gene Swiss-Prot Orphanet Ensembl
HGNC UniGene Nucleotide OMIM UCSC
Home Page
Symbol MYLK contributors: mct - updated : 24-08-2017
HGNC name myosin light chain kinase
HGNC id 7590
Corresponding disease
MMIH3 megacystis-microcolon-intestinal hypoperistalsis syndrome 3
TAAD8 thoracic aortic aneurysm with aortic dissection 8
Location 3q21.1      Physical location : 123.331.144 - 123.603.149
Synonym name
  • smooth muscle myosin light chain kinase
  • myosin, like chain kinase, Ca2+/calmodulin dependent, smooth muscle, non muscle MLC and KRP-related kinase (telokin) common gene
  • myosin, light polypeptide kinase
  • telokin
  • Synonym symbol(s) MLCK, FLJ12216, KRP, MLCK108, MLCK210, MSTP083, MYLK1, MLCK1, DKFZp686I10125, smMLCK
    TYPE functioning gene
    STRUCTURE 272.01 kb     34 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    regulatory sequence Promoter
    text structure at least two promoters
    MAPPING cloned Y linked N status confirmed
    Map cen - D3S1278 - D3S3529 - D3S3379 - D3S1558 - D3S3515 - D3S3513 - D3S3709 - D3S3576 - D3S1267 - D3S3645 - MYLK /D3S3552 - D3S3519 - D3S3646 - D3S1269 - D3S3569 - D3S1551 - qter
    regionally located not closely linked to olfactory receptor genes
    TRANSCRIPTS type messenger
    text smooth muscle MLCK
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    3 - 2679 - 154 - 2010 21055718
  • also called SM-MLCK or variant 8
  • 34 - 7852 210.7 1914 . adult, fetal tissues, nervous system (neurons, glia) and liver, lung, kidney, brain (hippocampus, entorhinal and frontal cortex), eye, aortic tissue . limited to well differentiated enterocytes, and this expression correlates closely with development of Na(+)-nutrient cotransport-dependent tight junction . in developing human heart 2010 21055718
  • also called variant 1 or non muscle MLCK1
  • full lenght of NM-MLCK
  • isoform responsible for tight junction regulation in absorptive enterocytes
  • elevated hydrostatic pressure in glaucomas, increased the expression MYLK-210 in astrocytes
  • 3 - 2676 17 153 some smooth muscles, eye not in aorta, brain 2010 21055718
  • alsos called KRP or variant 7
  • encodes the shorter isoform of kinase related protein, telokin
  • first exon corresponds to intron 30 and the remainder of the transcript corresponds to the last two exons of the gene
  • elevated hydrostatic pressure in glaucomas, increased the expression MYLK-130 in astrocytes
  • noncatalytic protein product that affects calcium sensitivity of contraction, primarily in intestinal smooth muscle
  • 33 - 7645 203.1 1845 adult, fetal tissues 2010 21055718
  • also called MLCK2 or variant 2
  • internal deletion
  • 33 - 7699 205.1 1863 adult, fetal tissues 2010 21055718
  • also called MLCK3a/b or variant 3A
  • internal deletion
  • does not utilize exon 30
  • 32 - 7492 197.4 1794 adult, fetal tissues 2010 21055718
  • also called MLCK4 or variant 3B
  • internal deletion
  • does not utilize exons 11 and 30
  • 33 - 7644 - 1738 - 2010 21055718
    33 splicing - - - highly expressed in vascular endothelial cells (EC) 2017 27529643
  • lacking exon 11
  • associated with reduced EC barrier integrity
    Type ubiquitous
       expressed in (based on citations)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart   highly
    Digestiveesophagus   highly
     intestinesmall intestine  highly
    Reproductivemale systemprostate  highly
    Respiratoryrespiratory tracttrachea  highly
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    SystemCellPubmedSpeciesStageRna symbol
    cell lineage
    cell lines
    at STAGE
  • N-terminus of the long MLCK inducing a disruption in normal spindle morphology and metaphase arrest
  • three C2-type Ig-like domains
  • a kinase domain
  • a regulatory Ca2+ binding domains
  • protein kinase superfamily
  • CAMK Ser/Thr protein kinase family
  • CATEGORY enzyme
    text at the intercalated discs of cardiac myocytes
    basic FUNCTION
  • perijunctional myosin light chain kinase, stimulating myosin ATPase activity without phosphorylating myosin light chain
  • key enzyme in muscle contraction
  • has an unexpected regulatory function during mitosis
  • important kinase required for phosphorylation of the regulatory light chain (RLC) of myosin, leading to its activation and subsequent interaction with actin filaments
  • activation of perijunctional myosin light chain kinase (MLCK) is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria
  • its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria
  • is a key regulator involved in endothelial hyperpermeability, and IL1B has been shown to mediate MYLK-dependent barrier dysfunction in epithelia
  • critical role in cell migration involves regulating the cell membrane tension and protrusion necessary for migration, thereby stabilizing the membrane skeleton through F-actin-binding activity
  • plays a crucial role in artery contraction, which regulates blood pressure and blood flow distribution
  • contributes to Ca(2+) flux regulation in vascular smooth muscle (VSM) and in non-muscle cells, where cytoskeleton has been suggested to help Ca(2+) channels trafficking
  • likely is linked to timely blastocyst formation, though it is dispensable for oocyte meiotic maturation
  • involved in isoform-specific non-muscle and smooth muscle contraction and regulation of vascular permeability during inflammation
  • vital participant in regulating vascular barrier responses to mechanical and inflammatory stimuli
  • key regulator of cell motility
  • important kinase required for myosin activation and subsequent interaction with actin filaments
    a component
    small molecule
  • phosphorylating myosin light chain
  • interacting with TJP1 (MYLK activity is critical to TJP1, but not claudin-1, occludin, or actin) exchange at the tight junction)
  • CLDN2 regulates colorectal inflammation via MYLK
  • FOXF1 directly binds to and activates the telokin (MYLK) promoter
  • NFKB1 is a novel partner of MYLK within endothelial cells
  • cell & other
    Other Ca2+/calmodulin dependent
    corresponding disease(s) TAAD8 , MMIH3
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    constitutional     --low  
    in aortic dissection
  • to familial thoracic aortic aneurysms leading to acute aortic dissections
  • to acute lung injury (ALI) and asthma
  • Variant & Polymorphism other
  • heterozygous loss-of-function mutations in MYLK are associated with aortic dissections
  • SNPs (P21H, S147P, V261A) alter the N-terminal amino acid sequence of the non-muscle isoform of MLCK (nmMLCK) and are highly associated with susceptibility to acute lung injury (ALI) and asthma
  • Candidate gene
  • hypermethylated BRINP3 and MYLK can be used as potential biomarkers for diagnosis and pre-warning of gastric cancer
  • Therapy target
    neurosensorialvisualanterior chamber
    may be a new target for intervention in glaucoma to alter reactive astrocyte migration in the optic nerve head