Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

MLF1IP

contributors: mct - updated : 22-08-2011

HGNC name	MLF1 interacting protein
HGNC id	21348

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	4q35.1      Physical location : 185.615.219 - 185.655.286
Genatlas name	myeloid leukemia factor-1 interacting protein
Synonym name	KSHV latent nuclear antigen interacting protein 1
	centromere protein U
	polo-box-interacting protein
Synonym symbol(s)	CENPU, KLIP1, PBIP1, CENP-U, CENP50, CENP-50, FLJ23468, CENP-U(50)

DNA

TYPE	functioning gene
STRUCTURE	40.07 kb     13 Exon(s)

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_024629 13 - 2554 - 418 - 2011 21056971

EXPRESSION

Type	ubiquitous

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Lymphoid/Immune thymus       highly Reproductive male system testis     highly Urinary bladder       highly

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Blood / hematopoietic bone marrow

cells

System Cell Pubmed Species Stage Rna symbol Blood/Hematopoietic erythroblast

cell lineage

cell lines

fluid/secretion

at STAGE

physiological period

fetal

Text

liver

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains	N-terminal bipartite nuclear localization signal (NLS) was responsible for nucleolar targeting
	two nuclear receptor-binding motifs (LXXLL),
	two leucine zippers,
	two PEST residues and several potential phosphorylation sites
	C-terminal half (AAs 200–418) is sufficient to form a heteromeric complex with CENPQ

HOMOLOGY

Homologene

FAMILY

CATEGORY

structural protein

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,cytoplasm,cytoskeleton,microtubule
	intracellular,nucleus,nucleoplasm
	intracellular,nucleus,nucleolus
text	localize in both the nucleus and the cytoplasm (Suzuki 2007)
	ectopically expressed MLF1IP was localized mainly in the nucleolus (who co-localized with fibrillarin) and nucleoplasm
	microtubule binding protein

basic FUNCTION	transcriptional repressor playing a role in suppressing red cell maturation, and a possible role in the genesis of erythroleukemias
	may have multi-functions and its different localizations may contribute to carcinogenesis (Suzuki 2007)
	constitutive centromere component essential for proper chromosome segregation
	important role in kinetochore-microtubule attachment through its interaction with NDC80
	forms a binary complex with CENPQ, to promote their mutual stability and subcellular localization to interphase and mitotic centromeres
	MLF1IP and CENPQ are mutually required to localize to interphase and mitotic centromeres

CELLULAR PROCESS

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

INTERACTION

DNA

RNA

small molecule

protein	interacting partner of NDC80, an evolutionarily conserved kinetochore core component essential for chromosome plasticity
	substrate of Aurora-B (phosphorylation of MLF1IP leads to reduced kinetochore-microtubule interaction, which contributes to the error-correcting function of Aurora-B)
	directly interacts with CENPQ, and this interaction was mutually required not only for their stability but also for their centromere localization
	bridges the interaction between PLK1 and CENPQ, and MLF1IP-bound PLK1 phosphorylates CENPQ

cell & other

REGULATION

ASSOCIATED DISORDERS

corresponding disease(s)

Other morbid association(s)

Type Gene Modification Chromosome rearrangement Protein expression Protein Function tumoral       loss of function in erythroleukemia tumoral     --over   in glioma and potentially in other types of malignancies

Susceptibility

Variant & Polymorphism

Candidate gene

Marker

Therapy target

ANIMAL & CELL MODELS