Selected-GenAtlas references	SOURCE	GeneCards	NCBI Gene	Swiss-Prot	Ensembl
	HGNC	UniGene	Nucleotide	OMIM	UCSC

Home Page

FLASH GENE

Symbol

MCM10

contributors: sge/npt/pgu - updated : 11-01-2010

HGNC name	minichromosome maintenance complex component 10
HGNC id	18043

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	10p13      Physical location : 13.203.553 - 13.253.100
Synonym name	homolog of yeast MCM10
	minichromosome maintenance deficient 10 (S. cerevisiae)
Synonym symbol(s)	PRO2249, CNA43, MGC126776, DNA43

DNA

TYPE	functioning gene
STRUCTURE	49.52 kb     20 Exon(s)

10 Kb 5' upstream gene genomic sequence study

regulatory sequence	Binding site   transcription factor
text structure	E2F binding motifs

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_182751 20 - 4535 NP_877428 98 875 - 2008 17997981 NM_018518 20 - 4532 NP_060988 - 874 - 2008 17997981

EXPRESSION

Type	restricted

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Digestive salivary gland         Nervous brain         Reproductive male system prostate

tissue

System Tissue Tissue level 1 Tissue level 2 Level Pubmed Species Stage Rna symbol Connective bone

cell lineage
cell lines
fluid/secretion	lymph

at STAGE

cell cycle

cell cycle, interphase, S, G2, M, checkpoint, G1S

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

zinc finger like motif

mono polymer

hexamer

HOMOLOGY

interspecies	homolog to S.cerevisae Mcm10
	homolog to Drosophila Mcm10
	homolog to rattus troponin T

Homologene

FAMILY

MCM10 family

CATEGORY

chaperone/stress , DNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleolus
text	early to mid-S phase, localized in discrete nuclear foci, in early S phase, several hundred foci appeared throughout the nucleus, in mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions, in the late S and G phases, localized to nucleoli

basic FUNCTION	temporarily recruited to the replication sites 30-60 min before they replicate and that it dissociates from chromatin after the activation of the prereplication complex
	required for forming the pre-initiation complex for initiation of chromosome replication and is involved in the elongation step
	playing an essential role for the efficient elongation step of chromosome replication
	being essential for maintaining genome integrity as well as cell cycle progression
	regulates both replication initiation and elongation and maintains genome integrity
	essential eukaryotic protein required for the initiation and elongation phases of chromosomal replication and for the association of several replication proteins, including POLA1, with chromatin
	might use a handoff mechanism to load and stabilize POLA1 within the replication fork
	required for chromatin loading of WDHD1 and an antibody that disrupts the MCM10-WDHD1 interaction interferes with the loading of WDHD1 and of POLA1, inhibiting DNA synthesis

CELLULAR PROCESS	cell cycle, progression
	nucleotide, replication
	nucleotide, genomic integrity

PHYSIOLOGICAL PROCESS

PATHWAY

metabolism

signaling

a component

MCM10 is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers

INTERACTION

DNA

RNA

small molecule	metal binding,
	Zn2+

protein	binding to replication factors
	interacting with POLA1
	WDHD1 interacts with MCM10, which associates with MCM2-7, and with the p180 subunit of POLA
	interacting directly with RECQL4 and regulating its unwinding activity
	MCM10 and CDC45 directly interact and establish a mutual co-operation in DNA binding

cell & other

binding to nuclease-resistant nuclear structures during S phase exclusively

REGULATION

activated by

MYCN

Other

regulated by proteolysis and phosphorylation in a cell cycle-dependent manner

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS