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FLASH GENE
Symbol LRWD1 contributors: mct - updated : 30-01-2014
HGNC name leucine-rich repeats and WD repeat domain containing 1p
HGNC id 21769
Location 7q22.1      Physical location : 102.105.389 - 102.113.610
Synonym name
  • centromere protein 33
  • ORC-associated protein
  • Synonym symbol(s) ORCA, CENP-33
    DNA
    TYPE functioning gene
    STRUCTURE 8.28 kb     15 Exon(s)
    regulatory sequence Promoter
    Binding site   transcription factor
    text structure
  • a 198 bp segment upstream of the LRWD1 transcription initiation site exhibited promoter activity. The LRWD1 core promoter lacked a TATA box but contained a NFKB binding site
  • core promoter lacked a TATA box but contained a NFKB binding site
  • MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    15 - 2220 - 647 - -
    EXPRESSION
    Type
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Reproductivemale systemtestis  specific SELECTED Mus musculusAdult
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Reproductivespermatid Mus musculus
    Reproductivespermatocyte Mus musculus
    Reproductivespermatozoa Mus musculus
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a leucine-rich repeat
  • a WD40 repeat domain, interacting with the origin replication complex (ORC), a protein complex involved in both initiation of DNA replication and heterochromatin silencing
  • HOMOLOGY
    interspecies homolog to murine Lrwd1
    Homologene
    FAMILY
    CATEGORY DNA associated
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,nucleus,chromatin/chromosome,heterochromosome
    intracellular,nucleus,chromatin/chromosome,centromere
    text centrosomal localization
    basic FUNCTION
  • may play an important role in spermatogenesis
  • is recruited to pericentric heterochromatin through binding to H3K9me3 and the association of LRWD1 with pericentric heterochromatin is required for heterochromatin silencing and maintenance
  • depletion of LRWD1 and ORC2 leads potentially to increased transcription of major satellite repeats
  • testicular-enriched protein that colocalizes with the centrosome at the base of sperm tail
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • LRWD1-mediated association of ORC1, ORC2 to chromatin is critical to initiate preRC assembly in G1 and chromatin organization in post-G1 cells
  • directly interacts with the N terminus of ORC2, and the stability of LRWD1 is dependent on its association with ORC2
  • associates with CDT1 and its inhibitor, geminin
  • LRWD1 associates with ORC2 throughout the cell cycle, with Cdt1 during mitosis and G(1), and with geminin in post-G(1) cells
  • WD repeat containing protein ORC-associated
  • ORC2 associates only with the non-ubiquitinated form of LRWD1, and ORC2 depletion results in the proteasome-mediated destabilization of LRWD1
  • NFKB is an important regulator for the expression of LRWD1
  • cell & other
    REGULATION
    Other is ubiquitinated at the WD40 repeat domain, a region that is also recognized by ORC2
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS