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FLASH GENE
Symbol LIMA1 contributors: mct/shn - updated : 26-04-2013
HGNC name LIM domain and actin binding 1
HGNC id 24636
Location 12q13.12      Physical location : 50.569.564 - 50.677.353
Genatlas name EPLIN
Synonym name
  • sterol regulatory element binding protein 3
  • epithelial protein lost in neoplasm beta
  • LIM domain and actin-binding protein 1
  • Synonym symbol(s) SREBP3, EPLIN, FLJ38853, MGC131726, PP624
    DNA
    TYPE functioning gene
    STRUCTURE 107.79 kb     11 Exon(s)
    motif repetitive sequence
    MAPPING cloned Y linked N status provisional
    Map cen - D12S1590 - D12S1627 - LIMA1 - D12S1635 - D12S361 - qter
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    11 splicing 3745 - 759 expressed in epithelial and endothelial cells in a context-dependent manner 2000 10806352
  • eplin beta
  • full length
  • 11 splicing initiation site 3748 - 760 - 2000 10806352
    initiation start site in intron 3
    8 splicing 3629 - 600 expressed in epithelial and endothelial cells in a context-dependent manner 2000 10806352
  • eplin alpha
  • cytoskeletal protein whose expression is often lost or is aberrant in cancerous cells and tissues and whose loss is believed to be involved in aggressive phenotype
  • tumour suppressor of oesophageal cancer
  • 5 splicing 3356 - 457 - 2000 10806352
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart   lowly Mus musculusAdult NM_001113545.1
     heart   moderately Mus musculusAdultNM_023063.3
    Digestiveliver   highly Mus musculusAdultNM_023063.3
     liver   moderately Mus musculusAdultNM_001113545.1
    Lymphoid/Immunespleen   highly Mus musculusAdultNM_001113545.1
     spleen   moderately Mus musculusAdultNM_023063.3
    Nervousbrain   moderately Mus musculusAdultNM_001113545.1
     brain   moderately Mus musculusAdultNM_023063.3
    Reproductivemale systemtestis  moderately Mus musculusAdultNM_001113545.1
     male systemtestis  highly Mus musculusAdultNM_023063.3
    Respiratorylung   highly Mus musculusAdultNM_023063.3
     lung   highly Mus musculusAdultNM_001113545.1
    Urinarykidney   lowly Mus musculusAdultNM_001113545.1
     kidney   highly Mus musculusAdultNM_023063.3
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal moderately Mus musculusAdultNM_001113545.1
    Muscularstriatum  highly Mus musculusAdultNM_023063.3
    cell lineage preferentially expressed in human epithelial cells of oral mucosa, prostate and mammary glands
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a single LIM/double zinc finger motif in the central region
  • two actin binding sites
  • HOMOLOGY
    interspecies ortholog to Lima1, Mus musculus
    ortholog to lima1, Danio rerio
    ortholog to Lima1, Rattus norvegicus
    ortholog to LIMA1, Pan troglodytes
    Homologene
    FAMILY LIM domain protein
    CATEGORY tumor suppressor
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,cytoskeleton,microfilament
    text
  • expressed at similar levels in endothelial and epithelial cells and is located at interendothelial junctions in confluent cells
  • basic FUNCTION
  • promotes the formation of stable actin filament structures such as stress fibers at the expense of more dynamic actin filament structures such as membrane ruffles
  • functions to link the cadherin-catenin complex to F-actin and simultaneously stabilizes this population of actin fibers, resulting in the establishment of the adhesion belt
  • actin-binding protein that functions as a key molecule linking the cadherin-catenin complex to the actin cytoskeleton
  • may function during cytokinesis to maintain local accumulation of key cytokinesis proteins at the furrow
  • cytoskeletal target gene transcriptionally regulated by the actin-MKL1-SRF pathway, which supports a role in cancer biology
  • could function as a suppressor of tumor metastasis by negatively regulating EMT in Prostate carcinoma cells
  • may contribute to the motility of invasive tumor cells
  • negative regulator of epithelial-mesenchymal transition and invasiveness in prostate cancer cells
  • functions to maintain the zonula adherens via its association with CTNNA1, but was lost in the punctate adherens junctions
  • epithelial cells remodel their junctional architecture by responding to mechanical forces, and CTNNA1-bound LIMA1 acts as a mechanosensitive regulator for this process
  • establishes a link between the cadherin·catenin complex and actin that is independent of actomyosin tension
  • necessary for stabilization of capillary structures in an angiogenesis model
  • EPLIN-&
  • 945; is a tumour suppressor of oesophageal cancer
    CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • Supervillin
  • interacts with CTNNA1 and tethers the VE-cadherin·catenin complex to the actin cytoskeleton
  • alpha-cetenin and actin filaments
  • EGF activates ERK1/2-dependent phosphorylation, ubiquitination, and protein turnover of LIMA1
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --low  
    EPLIN-alpha is expressed at lower levels in oesophageal cancer tissues
    tumoral     --low  
    in prostate carcinoma
    constitutional     --over  
    its over-expression can regulate in HECV endothelial cells motility, matrix adhesion and tubule formation
    tumoral     --low  
    could significantly contribute to the progression of epithelial cancer toward metastatic status
    Susceptibility
  • loss of EPLIN seen in cancer cells may play a role in cancer progression
  • Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • EPLIN-depleted endothelial cells exhibited a reduced capacity to form pseudocapillary networks because of numerous breakage events