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FLASH GENE
Symbol GTF2F1 contributors: mct - updated : 10-10-2012
HGNC name general transcription factor IIF, polypeptide 1, 74kDa
HGNC id 4652
Location 19p13.3      Physical location : 6.379.579 - 6.393.291
Synonym name
  • RNA polymerase associated protein, RAP74
  • general transcription factor IIF, polypeptide 1 (74kD subunit)
  • transcription initiation factor RAP74
    • Synonym symbol(s) RAP74, TF2F1, BTF4, TFIIF
    DNA
    TYPE functioning gene
    STRUCTURE 13.71 kb     13 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status confirmed
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    13 - 2551 58.2 517 - 2004 15351637
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Endocrineadrenal gland   highly
     thyroid   highly
    Nervousnervecranial nerve  highly
    Visualeye   highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectivebone  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a central charged cluster domain (binding to SRF's activation domain)
    • mono polymer heteromer , dimer
    HOMOLOGY
    interspecies homolog to C.elegans Co1f1.1
    Homologene
    FAMILY
  • TFIIF alpha subunit family
    • CATEGORY transcription factor
    SUBCELLULAR LOCALIZATION     plasma membrane,junction
        intracellular
    intracellular,nucleus,nucleoplasm
    basic FUNCTION
  • transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB
  • it promotes transcription elongation
  • increasing the average rate of RNA chain elongation across DNA templates
  • required for transcriptional initiation by RNA polymerase II
  • supports elongation and suppresses pausing by stabilizing the post-translocated elongation complex, in collaboration with TCEA1 (TCEA1 has a role in suppression of transient pausing)
  • with POLR2G are involved in both early and late transcriptional stages (Cojocaru 2008)
    • CELLULAR PROCESS nucleotide, transcription, initiation
    nucleotide, transcription, elongation
    nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • heterodimer of an alpha and a beta subunit
  • subunit of RNA polymerase II transcription initiation factor II F
    • INTERACTION
    DNA
    RNA
    small molecule cofactor,
  • Mg2+
    • protein
  • RNA polymerase II, SRF (serum response factor)
  • interacts with GTF2F2 and CTDP1
  • has an important role in stabilizing GTF2B within the preinitiation complex and after transcription initiates
  • importance of GTF2F1 in stabilizing GTF2B interaction with the transcription complex, and potentially GTF2F1 should also participate in the process of elongation termination
  • POLR2M stabilizes poised polymerases while maintaining their responsiveness to CCNT1, suggesting that Mediator overcomes a POLR2M-mediated block of initiation by allowing GTF2F1 function
  • POLR2M competes with GTF2F1 for binding to the RPB1 and RPB5 subunits of Pol II, thereby inhibiting an essential function of GTF2F1 in preinitiation complex assembly
    • cell & other
    REGULATION
    Other critical target for SRF-activated transcription
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS