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FLASH GENE
Symbol GORASP1 contributors: mct/npt/pgu - updated : 28-11-2016
HGNC name Golgi reassembly stacking protein 1, 65kDa
HGNC id 16769
Location 3p22.2      Physical location : 39.138.090 - 39.149.130
Synonym name
  • Golgi peripheral membrane protein p65
  • Golgi reassembly stacking protein
  • Golgi phosphoprotein 5
  • Synonym symbol(s) P65, GRASP65, FLJ23443, GOLPH5
    DNA
    TYPE anonymous DNA segment
    STRUCTURE 11.76 kb     9 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    Physical map
    LOC391528 3 similar to ribosomal protein L18a; 60S ribosomal protein L18a SCN5A 3p21 sodium channel, voltage-gated, type V, alpha (long QT syndrome 3) SCN10A 3p21-p22 sodium channel, voltage-gated, type X, alpha SCN11A 3p24-p21 sodium channel, voltage-gated, type XI, alpha KIAA1449 3p21.33 WD repeat endosomal protein GORASP1 3p22-p21.33 golgi reassembly stacking protein 1, 65kDa STI2 3p21.33 TPR domain containing STI2 AXUD1 3p22 AXIN1 up-regulated 1 CMYA1 3p21.33 cardiomyopathy associated 1 CX3CR1 3p21.32-p21.31 chemokine (C-X3-C motif) receptor 1 CCR8 3p21.32-p21.31 chemokine (C-C motif) receptor 8 hnRNPA1p 3p21.33 heterogeneous nuclear ribonucleoprotein A1 pseudogene LOC389109 3 similar to elongation factor 1 alpha LOC389110 3 similar to Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu) LOC389111 3 similar to RIKEN cDNA 1110038F21 FLJ20551 3p21.33 hypothetical protein FLJ20551 LAMR1 3p21.3 laminin receptor 1 (ribosomal protein SA, 67kDa) MOBP 3p22-p21.33 myelin-associated oligodendrocyte basic protein
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    9 - 3789 65 440 - 2005 15888544
    6 - 2861 - 285 - 2005 15888544
    7 - 3041 - 345 - 2005 15888544
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Lymphoid/Immunethymus   highly
    Nervousnervecranial nerve  highly
    Reproductivefemale systemovary  highly
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Connectivebone  highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • N-terminal GRASP domain (amino acids 1-201) with two PDZ domains necessary and sufficient for dimerization and trans-oligomerization but not mitotically regulated (PDZ2 targets the molecule to cis Golgi membranes by binding a PDZ ligand present at the C terminus of cis-localized GOLGA2, whereas PDZ1 likely binds a similarly targeted GORASP1 molecule on an adjacent membrane)
  • two PDZ-like domains, responsible for oligomerization and Golgi membrane stacking, followed by a variable C-terminal region
  • and a zinc finger-like structure is observed in the linker region between the PDZ domains
  • presence of a PLK1 phosphorylation site, Ser189, next to the PDZ ligand sequence suggests several possible inhibitory mechanisms
  • C-terminal serine/proline-rich domain (amino acids 202-446) cannot dimerize nor can it link adjacent surfaces, but confer mitotic regulation on the GRASP domain through multiple sites phosphorylated by the mitotic kinases
  • conjugated PhosphoP
    HOMOLOGY
    Homologene
    FAMILY
  • Golgi reassembly stacking protein family
  • CATEGORY structural protein
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytosolic
    text
  • tightly bound to Golgi membrane
  • localize to the cis and medial-trans cisternae
  • localized mainly on the cis-side of the Golgi (23940043)
  • basic FUNCTION
  • may function as a signal integrator controlling the cell growth
  • involved in establishing the stacked structure of the Golgi apparatus
  • having a role in the regulation of spindle dynamics rather than a direct role in the stacking of Golgi cisternae
  • implicated in the stacking of Golgi cisternae and the regulation of Golgi disassembly/reassembly during mitosis
  • required for Golgi fragmentation during mitosis and mitotic progression in both yeast and mammalian cells
  • required for unconventional protein secretion during development
  • stack mammalian Golgi cisternae via a common mechanism
  • Golgi matrix proteins having recognized roles in maintaining the architecture of the Golgi complex, in mitotic progression and in unconventional protein secretion
  • having a role in the transport of selected cargo along the conventional secretory pathway
  • with GOLGB1, and GOLGA2, facilitates vesicle fusion to
  • the Golgi membrane as a vesicle "tethering factor"
  • GORASP1, GORASP2 are required for formation of the Golgi ribbon, a structure which is fragmented in mitosis owing to the phosphorylation of a number of serine and threonine residues situated in its C-terminus
  • also seems to be the key target of signalling events leading to re-orientation of the Golgi during cell migration and its breakdown during apoptosis
  • GORASP1, GORASP2 are negative regulators of exocytic transport and this slowdown helps to ensure more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi network
  • direct role in Golgi stacking
  • GORASP1, GORASP2 play a direct and cisternae-specific role in linking ministacks into a continuous membrane network
  • GORASP1, GORASP2 specifically link analogous cisternae to ensure Golgi compartmentalization and proper processing
  • implicated in both Golgi stacking and ribbon linking by forming trans-oligomers
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    text Golgi apparatus biosynthesis
    PATHWAY
    metabolism
    signaling
    a component complexing with GOLGA2 (complex binds to the vesicle docking protein p115, VDP/p115)
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • GM130
  • caspase-3
  • PLK1 binds the GORASP1 C-terminal domain under mitotic conditions
  • mitotic phosphorylation of the conserved GRASP domain of GORASP1 disrupts its self-association, leading to a loss of Golgi membrane tethering, cisternal unlinking, and Golgi breakdown
  • DNAJA1 is a novel GORASP1-binding protein
  • cell & other
    REGULATION
    Other likely phosphorylated on it regulatory domain to create a PLK1 docking site to promote PLK1 phosphorylation of Ser189, which blocks the internal PDZ ligand from binding the PDZ1 groove in another molecule
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS