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FLASH GENE
Symbol EPPIN contributors: mct - updated : 14-02-2018
HGNC name epididymal peptidase inhibitor
HGNC id 15932
Location 20q13.12      Physical location : -
Genatlas name eppin
Synonym name
  • serine protease inhibitor-like, with Kunitz and WAP domains 1 (eppin)
  • WAP four-disulfide core domain protein 7
  • cancer/testis antigen 71
  • cancer/testis antigen 72
  • protease inhibitor WAP7
  • Synonym symbol(s) EPPIN1, EPPIN2, EPPIN3, EPPI, WAP7, SPINLW1, CT71, CT72, dJ461P17.2
    DNA
    TYPE functioning gene
    STRUCTURE 6.80 kb     4 Exon(s)    1 Copie(s)
    regulatory sequence Promoter (TATA box)
    Binding site   enhancer
    text structure number of putative transcription enhancer binding sites typical of genes expressed in the epididymis or testis
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    4 splicing 800 15 127 epididymis and testis only 2013 24358212
  • EPPIN1
  • same protein isoform than EPPIN3
  • - splicing 1200 - 117 epididymis only 2013 24358212
    4 splicing 1998 15.28 133 testis only 2013 24358212
  • EPPIN3
  • same protein isoform than EPPIN1
  • EXPRESSION
    Type restricted
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Nervousbrain     Rattus norvegicus
    Reproductivemale systemmale genital tractepididymis highly Rattus norvegicus
     male systemtestis  highly Rattus norvegicus
     male systemmale genital tractvas deferens   Rattus norvegicus
     male systemseminal vesicles    Rattus norvegicus
    Respiratorylung     Homo sapiensAdult
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Blood/Hematopoieticmonoblast Homo sapiens
    Blood/Hematopoieticmyelocyte Homo sapiens
    ReproductiveSertoli cell Rattus norvegicus
    Reproductivespermatocyte Mus musculus
    Reproductivespermatozoa Homo sapiens
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • two potential protease inhibitory domains: a Kunitz/bovine pancreatic trypsin inhibitor domain and a WAP-type (Whey Acidic protein) "four-disulfide core" domain (protease inhibitor concensus sequences)
  • HOMOLOGY
    Homologene
    FAMILY protease inhibitor family
    CATEGORY regulatory
    SUBCELLULAR LOCALIZATION extracellular
        plasma membrane
    basic FUNCTION
  • protease inhibitor
  • although both of EPPIN domains are involved in the protein's antibacterial activity, only the Kunitz domain is required for selective protease inhibition
  • EPPIN controls sperm motility in the ejaculate by binding SEMG1, resulting in the loss of calcium, most likely through a disturbance of internal pH and an inhibition of uptake mechanisms
  • EPPIN and SEMG1 rapidly co-evolved in primates due to their critical role in the modulation of sperm motility in the semen coagulum
  • modulates the liquefaction of human semen
  • EPPIN and FN1 bind to each other in human semen and on human ejaculated spermatozoa
  • is a serine protease inhibitor expressed in male reproductive tissues
  • potential role for eppin outside of the reproductive tract and eppin may have a role in the innate immune response in the lung
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • during ejaculation SEMG (semenogelin) from the seminal vesicles binds to the EPPIN protein complex, initiating a series of events that define EPPIN function
  • EPPIN is a key player in the protein-protein interactions within the EPPIN protein complex [EPC]
  • EPPIN's sequence C102-P133 contains the major binding site for SEMG1
  • physiological significance of the SEMG1/EPPIN interaction on the surface of spermatozoa is its capacity to modulate sperm progressive motility
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Susceptibility
    Variant & Polymorphism
    Candidate gene
  • EPPIN-FN1 complex may involve in semen coagulation, liquefaction and the survival and preparation of spermatozoa for fertility in the female reproductive tract
  • Marker
    Therapy target
    SystemTypeDisorderPubmed
    reproductioncontraception 
    target for male contraception because of its critical role in sperm motility
    ANIMAL & CELL MODELS