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FLASH GENE
Symbol EIF5 contributors: mct - updated : 19-02-2019
HGNC name eukaryotic translation initiation factor 5
HGNC id 3299
Location 14q32.32      Physical location : 103.800.492 - 103.811.359
Synonym symbol(s) EIF-5A
DNA
TYPE functioning gene
STRUCTURE 10.87 kb     12 Exon(s)
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked N status provisional
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
12 - 5809 49.1 431 - 2006 16584190
11 - 5782 49.1 431 - 2006 16584190
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Cardiovascularheart   highly
Digestivestomach   highly
Lymphoid/Immunethymus   highly
Reproductivemale systemtestis  highly
tissue
SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
Connectivebone   
cell lineage
cell lines
fluid/secretion
at STAGE
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • N-terminus functioning as a GTPase-activator protein (GAP) for GTP bound to eIF2
  • C terminal HEAT domain interacting with EIF1, EIF2S1, EIF3A in the multifactor complex and and with eIF4G1 in the 48S complex, nucleating the interactions between multiple translation factors, including eIF1, which acts to inhibit GTP hydrolysis or P(i) release, and the beta subunit of EIF2 , and C-terminal domain (CTD) stimulates 43S preinitiation complex (PIC) assembly
  • secondary structure
  • EIF5-C-Terminal domain is exclusively composed out of alpha-helices and is homologous to the carboxy-terminal domain of eIF2B-epsilon (eIF2Bepsilon-CTD)
  • HOMOLOGY
    Homologene
    FAMILY
  • eukaryotic translation initiation factor family
  • CATEGORY enzyme , transcription factor
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,cytosolic
    basic FUNCTION
  • hydrolyzing GTP of the 40S ribosomal initiation complex
  • plays multiple roles in translation initiation
  • stabilizes the binding of GDP to EIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI)
  • multifunctional protein with separate GDI (GDP dissociation inhibitor) and GAP (GTPase accelerating protein) activities, both necessary for controlled translation initiation in eukaryotes
  • C-terminal domain (CTD) of EIF5 stimulates 43S preinitiation complex (PIC) assembly
  • plays an important role in translation start site selection by providing the GAP (GTPase activating protein) function
  • levels of eukaryotic translation initiation factors 1 (EIF1) and 5 (EIF5) play critical roles in controlling the stringency of translation start site selection
  • key role of EIF5 in start-codon selection
  • CELLULAR PROCESS nucleotide, transcription, initiation
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • part of EIF2–EIF5 complex, a new critical component of the regulatory circuit that inhibits EIF2B GEF function
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • EIF5-CTD interacts directly with the translation initiation factors EIF1, EIF2S2, and EIF3C, thus forming together with EIF2S2 bound Met-tRNA(i)(Met) the multifactor complex (MFC)
  • role of EIF5-CTD dynamic interplay with EIF1 and EIF2S2 in switching PICs from an open to a closed state at start codons
  • C-terminal domain of EIF5 is responsible for the factor activity in antagonizing EIF1 binding to the preinitiation complex (PIC)
  • importance of EIF5 GDI (GDP-dissociation inhibitor) activity, and EIF2S2 acts in concert with EIF5 to prevent premature release of GDP from EIF2S3 and thereby ensure tight control of protein synthesis initiation
  • EIF5 interacts with EIF5B and may help recruit EIF5B to the PIC (an EIF5B-binding motif was identified at the C-terminus of eIF5, similar to that found in EIF1AX)
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS