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FLASH GENE
Symbol EDF1 contributors: mct - updated : 21-08-2013
HGNC name endothelial differentiation-related factor 1
HGNC id 3164
Location 9q34.3      Physical location : 139.756.570 - 139.760.738
Synonym name multiprotein bridging factor 1
Synonym symbol(s) MBF1, EDF-1
DNA
TYPE functioning gene
STRUCTURE 4.22 kb     6 Exon(s)
10 Kb 5' upstream gene genomic sequence study
regulatory sequence Promoter (CAAT box)
Binding site   transcription factor
text structure
  • two GC boxes
  • binding sites for SP1/SP3 and NFY
  • MAPPING cloned Y linked N status provisional
    Physical map
    LOC389811 9 similar to HSPC324 EGFL7 9q34.3 EGF-like-domain, multiple 7 AGPAT2 9q34.3 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) MGC20262 9q34.3 hypothetical protein MGC20262 MGC16037 9q34.3 hypothetical protein MGC16037 LCN6 9q34.3 lipocalin 6 LOC138307 LOC389812 LOC392401 9 similar to vacuolar H(+)-ATPase subunit; V-ATPase subunit; M16 MGC14141 9q34.3 hypothetical protein MGC14141 MGC15438 9q34.3 hypothetical protein MGC15438 FLJ10101 9q34.3 FLJ10101 protein LOC389813 9 similar to ENSANGP00000006371 PHP14 9q34.3 phosphohistidine phosphatase AEGP 9q34.3 apical early endosomal glycoprotein precursor EDF1 9q34.3 endothelial differentiation-related factor 1 TRAF2 9q34 TNF receptor-associated factor 2 FBXW5 9q34.3 F-box and WD-40 domain protein 5 C8G 9q22.33-q32 complement component 8, gamma polypeptide MGC48935 9q34.3 hypothetical protein MGC48935 LOC203235 9q34.3 hypothetical LOC203235 PTGDS 9q34.2-q34.3 prostaglandin D2 synthase 21kDa (brain) LOC286257 9q34.3 hypothetical protein LOC286257 CLIC3 9q34.3 chloride intracellular channel 3 ABCA2 9q34.3 ATP-binding cassette, sub-family A (ABC1), member 2 FUT7 9q34.3 fucosyltransferase 7 (alpha (1,3) fucosyltransferase) LOC389814 9 similar to LPAL6438 NPDC1 9q34.3 neural proliferation, differentiation and control, 1 ENTPD2 9q34 ectonucleoside triphosphate diphosphohydrolase 2 LOC89958 9q34.3 hypothetical protein LOC89958 LOC91373 MAN1B1 9q34 mannosidase, alpha, class 1B, member 1 DPP7 9q34.3 dipeptidylpeptidase 7 LOC389815 9 LOC389815 GRIN1 9q34.3 glutamate receptor, ionotropic, N-methyl D-aspartate 1 LOC389816 9 similar to CDNA sequence BC004853 LOC389817 9 LOC389817 ANAPC2 9q34.3 LOC389817 SSNA1 9q34.3 Sjogren's syndrome nuclear autoantigen 1 C9orf75 9q34.3 chromosome 9 open reading frame 75
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    5 - 701 16.2 148 - 2002 12040021
  • variant alpha
  • 4 splicing 700 15.1 139 - 2002 12040021
  • variant beta
  • differing in the 3'utr and coding region
  • 5 - 638 - 127 - 2002 12040021
    5 - 647 - 130 - 2002 12040021
    5 - 852 - 141 - 2002 12040021
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
    Endocrinepancreas    
    Nervousbrain    
    Respiratorylung    
    cells
    SystemCellPubmedSpeciesStageRna symbol
    Cardiovascularendothelial cell
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period fetal
    cell cycle     cell cycle
    Text kidney
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a N terminal region binding to the activators
  • a MBF1 C terminal domain for binding to TBP
  • HOMOLOGY
    interspecies homolog to murine Edf1 (98.6pc)
    homolog to chicken EDF1 (93.2pc)
    Homologene
    FAMILY
    CATEGORY signaling
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,nucleus,chromatin/chromosome
    intracellular,nucleus,nucleolus
    basic FUNCTION
  • bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery
  • transcriptional coactivator for transcription factors such as FTZ-F1,GCN4,SF1 (NR5A1)
  • recruiting TATA box binding proteins to promoters interactivators an bound
  • enhancing the DNA-binding activity of ATF1, ATF2, CREB1
  • regulating nitric oxid synthase activity by sequestering calmodulin in the cytoplasm
  • required for PPARG-stimulated adipogenesis
  • functions as a coactivator of several transcription factors
  • implicated in molecular events that are pivotal to endothelial function and, therefore, to vascular integrity
  • calmodulin binding protein that regulates calmodulin-dependent enzymes
  • inactivates the calmodulin/calcineurin/NFAT pathway via sequestration of calmodulin, during early adipogenesis
  • might have a complex role during adipose differentiation
  • CELLULAR PROCESS cell life, differentiation
    nucleotide, transcription, regulation
    PHYSIOLOGICAL PROCESS development
    text
  • repression of endothelial cells differentiation
  • angiogenesis
  • PATHWAY
    metabolism lipid/lipoprotein
    signaling
    EDF1/CALM1/PPP3CA/NFATC1 pathway
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with TBP, TFIID complex, NR5A2, NR1H3 and PPARG
  • binding NR5A1, ATF1, FOS and JUN
  • in endothelial cells, bound with CALM1, through its IQ domain sequestering this complex in the cytoplasm of endothelial cells
  • is a cofactor of PPARG
  • associates with calmodulin and calcineurin, most likely inactivating calcineurin
  • can associate in a protein complex with CALM1, and PPP3CA but not with NFATC1, suggesting that EDF1 is a key element in the CALM1/PPP3CA/NFATC1 pathway
  • cell & other
    REGULATION
    Other phosphorylated by PKA and PKC
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS