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FLASH GENE
Symbol ECT2 contributors: mct/npt/pgu - updated : 13-09-2014
HGNC name epithelial cell transforming sequence 2 oncogene
HGNC id 3155
Location 3q26.31      Physical location : 172.472.297 - 172.539.263
Synonym name protein ECT2
Synonym symbol(s) FLJ10461, MGC138291, ARHGEF31
DNA
TYPE functioning gene
STRUCTURE 66.96 kb     24 Exon(s)
10 Kb 5' upstream gene genomic sequence study
MAPPING cloned Y linked N status provisional
RNA
TRANSCRIPTS type messenger
identificationnb exonstypebpproduct
ProteinkDaAAspecific expressionYearPubmed
24 - 3916 - 883 - 2000 10837491
25 - 4422 - 883 - 2000 10837491
25 - 4192 - 914 - 2000 10837491
EXPRESSION
Type ubiquitous
   expressed in (based on citations)
organ(s)
SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
Digestiveesophagus   highly
 mouth   highly
Reproductivemale systemtestis  highly
cell lineage
cell lines
fluid/secretion
at STAGE
cell cycle     cell cycle, G2M
PROTEIN
PHYSICAL PROPERTIES
STRUCTURE
motifs/domains
  • two BRCT adaptor domain (BRCA1 C-terminus)
  • a DH(dbl-homology) domain
  • a PH domain
  • HOMOLOGY
    interspecies homolog to murine Ect2 proto-oncogene 2
    ortholog to ortholog of Drosophila pebble
    Homologene
    FAMILY
    CATEGORY secretory , protooncogene
    SUBCELLULAR LOCALIZATION     plasma membrane,junction,tight
        intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus
    text
  • its localization defines the stage-specific changes in actin cortex organization critical for accurate cell division
  • concentrates at the equatorial membrane during cytokinesis in live cells
  • basic FUNCTION
  • putative regulator of the Ras-related Rho subfamily of GTP binding proteins
  • having a critical role in Rho activation during cytokinesis
  • expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis
  • concentrates on the central spindle by binding to central spindlin
  • having function in polar lamellipodia formation and polarity maintenance during "contractile ring-independent" cytokinesis in adherent cells
  • can, in a dose-dependent manner, reduce multinucleation induced by GRLF1, and GRLF1 has opposing roles in regulating cytokinesis
  • plays a critical role in RHOA, RHOB activation during cytokinesis, and thus may play a role in the pathogenesis of pancreatic cancer
  • plays a role in cytokinesis, in which it regulates local activation of RHOA together with ECT2
  • PKP4 and ECT2 are transported via distinct motors, identifying a novel pathway implicating KIF3B in the regulation of actin organization during cytokinesis
  • guanine nucleotide exchange factor for Rho family GTPases, that links the PRKCI-PARD6A complex to RAC1 activation and cellular transformation
  • with PRKCI, are genetically and functionally linked in non-small cell lung cancer, acting to coordinately drive tumor cell proliferation and invasion through formation of an oncogenic PRKCI-PARD6A-ECT2 complex
  • nuclear guanine nucleotide exchange factors ECT2 and NET1 regulate RhoB-mediated cell death after DNA damage
  • an ANLN-ECT2 complex stabilizes central spindle microtubules at the cortex during cytokinesis
  • contributes to tight junction function in epithelial cells
  • is important for tight junction function and maintenance of cell polarity
  • is a central regulator of mitotic rounding, thus uncovering a link between the cell-cycle machinery that drives mitotic entry and its accompanying actin remodeling C
  • is a RhoGEF that plays a well-established role in formation of the actomyosin contractile ring at mitotic exit, through the local activation of RHOA
  • negatively regulates the Wingless/Wnt signaling pathway, and is a human proto-oncogene
  • cell cycle regulator that plays critical roles in cytokinesis
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • associates with RACGAP1 at anaphase and during cytokinesis
  • interaction with UBE2A (role in regulating growth of neuronal processes or synapse formation through the degradation or cellular localization of various proteins such as ECT2)
  • interacts with the polarity protein complex PARD6A/PRKCZ and regulates PRKCZ activity
  • interacting with ARHGEF11 (is required for cortical localization of RhoA and contractile ring assembly)
  • interacting with KLHL20
  • interacting with RACGAP1 and regulating the activation and function of CDC42 in mitosis (Oceguera-Yanez 2005)
  • full activation of RHOA required ECT2 as well as PKP4 indicating that these two proteins act in conjunction to regulate RHOA during cytokinesis
  • expression of ECT2, RACGAP1, and KIF23 is tightly regulated during cell cycle progression
  • CUX1 and E2F1 regulate coordinated expression of the mitotic complex genes ECT2, RACGAP1, and KIF23 in S phase
  • at anaphase, binding to RACGAP1 at the spindle midzone repositions ECT2 to induce local actomyosin ring formation
  • interaction between ECT2 and KRT4 through the second ECT2 BRCT domain
  • ECT2 binds to RACGAP1 via its N-terminal BRCT domain (BRCA1 C-terminal), which docks at specific phosphorylated residues
  • cell & other
    REGULATION
    Phosphorylated by PRKCI (oncogenic activity of ECT2 is regulated through protein kinase C iota-mediated phosphorylation)
    Other regulated by centralspindlin with RHOA accumulation at the equatorial cortex during cytokinesis
    regulated by PRKCI-PARD6A complex (regulates the cytoplasmic localization of ECT2)
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Other morbid association(s)
    TypeGene ModificationChromosome rearrangementProtein expressionProtein Function
    tumoral     --over  
    in pancreatic tumor tissues
    tumoral   amplification    
    in invasive lung adenocarcinoma
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS