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FLASH GENE

Symbol

DIS3

contributors: mct - updated : 08-07-2013

HGNC name	DIS3 mitotic control homolog (S. cerevisiae)
HGNC id	20604

FLASH GENE DNA RNA EXPRESSION PROTEIN DISORDER ANIMAL & CELL MODELS

Location	13q22.1      Physical location : 73.329.541 - 73.356.344
Synonym name	related to S pombe mitotic control protein DIS3
	mitotic control protein dis3 homolog
	ribosomal RNA processing protein 44
	exosome complex exonuclease RRP44
Synonym symbol(s)	RRP44, dis3p, KIAA1008, FLJ10484, MGC33035, RP11-342J4.3, bA555G22.1, DKFZp667L1817
EC.number	3.1.13.-

DNA

TYPE	functioning gene
STRUCTURE	26.80 kb     21 Exon(s)

10 Kb 5' upstream gene genomic sequence study

MAPPING

cloned

Y

linked

N

status

provisional

RNA

TRANSCRIPTS	type	messenger

identification nb exons type bp product Protein kDa AA specific expression Year Pubmed NM_014953 21 - 7667 NP_055768 109 958 - 2007 17643380 NM_001128226 21 - 7577 NP_001121698 - 928 - 2007 17643380

EXPRESSION

Type

widely

expressed in	(based on citations)
organ(s)	System Organ level 1 Organ level 2 Organ level 3 Organ level 4 Level Pubmed Species Stage Rna symbol Cardiovascular vessel       highly Digestive intestine large intestine colon   moderately   stomach       moderately Lymphoid/Immune lymph node       predominantly Reproductive male system testis     highly Urinary kidney       moderately

cell lineage

cell lines

fluid/secretion

at STAGE

PROTEIN

PHYSICAL PROPERTIES

STRUCTURE

motifs/domains

N-terminal PIN domain necessary and sufficient for association with the core exosome, indicating its dual function as a nuclease and structural element

mono polymer

complex

HOMOLOGY

interspecies

homolog to S pombe mitotic control protein DIS3

intraspecies

paralog to DIS3L2, DIS3L

Homologene

FAMILY

ribonuclease II (RNB) family

CATEGORY

enzyme , RNA associated

SUBCELLULAR LOCALIZATION	intracellular
	intracellular,nucleus,nucleolus
text	exosome (RNase complex)
	nuclear localization

basic FUNCTION	required for the 3' processing of the 7S pre-RNA to the mature nuclear complex
	having a 3'-5' exonuclease activity
	RNase activity of DIS3, a core subunit of exosome, was found to be required for proper kinetochore formation and establishment of kinetochore-microtubule interactions
	contribute potentially to kinetochore formation through an involvement in heterochromatic silencing at both outer centromeric repeats and within the central core region
	DIS3 and DIS3L are active exonucleases, but, only DIS3 has retained endonucleolytic activity
	DIS3L2, DIS3 and DIS3L, have exoribonuclease activity and bind to the core RNA exosome complex
	can processively digest structured RNA one nucleotide at a time in the 3prime to 5prime direction
	accumulates the energy released by multiple single nucleotide step hydrolysis reactions until about four base pairs are unwound in a burst
	ribonuclease that acts directly in the processing, turnover, and surveillance of a large number of distinct RNA species
	required for the development of a multicellular organism

CELLULAR PROCESS	cell cycle, division, mitosis
	nucleotide

PHYSIOLOGICAL PROCESS

text	rRNA processing

PATHWAY

metabolism

signaling

a component	component of the exosome 3'->5' exoribonuclease complex
	catalytic subunit of the exosome

INTERACTION

DNA

RNA

binding

small molecule

protein	GTPase Ran
	association of DIS3 and DIS3L--with the human exosome core

cell & other

REGULATION

ASSOCIATED DISORDERS

ANIMAL & CELL MODELS