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FLASH GENE
Symbol CLASP2 contributors: npt/shn/pgu - updated : 09-12-2015
HGNC name cytoplasmic linker associated protein 2
HGNC id 17078
Location 3p22.3      Physical location : 33.537.740 - 33.759.848
Synonym name
  • cDNA sequence,expressed in brain, >100kDa, similar to KIAA0622 related to C elegans 117.3 kDa protein
  • CLIP-associating protein 2
  • multiple asters (Mast)-like homolog 2
  • protein Orbit homolog 2
  • Synonym symbol(s) KIAA0627, hOrbit2
    DNA
    TYPE functioning gene
    STRUCTURE 222.11 kb     38 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    Map pter - D3S2432 - D3S4525 - CLASP2 - D3S3518 - D3S1619 - cen
    Physical map
    SIMP 3p22.3 source of immunodominant MHC-associated peptides OSBPL10 3p22.3 oxysterol binding protein-like 10 LOC344787 3p22.3 similar to KIAA2033 protein KIAA0089 3p22.3 similar to KIAA2033 protein LOC391525 3 similar to 40S ribosomal protein SA (P40) (34/67 kDa laminin receptor) (Colon carcinoma laminin-binding protein) (NEM/1CHD4) (Multidrug resistance-associated protein MGr1-Ag) CKLFSF8 3p22.3 chemokine-like factor super family 8 CKLFSF7 3p22.3 chemokine-like factor super family 7 CKLFSF6 3p22.3 chemokine-like factor super family 6 LOC285260 3p22.3 similar to ribosomal protein L31 DNCLI1 3p22.2 dynein, cytoplasmic, light intermediate polypeptide 1 LOC391526 3 similar to Immunoglobulin-binding protein 1 (CD79a-binding protein 1) (B cell signal transduction molecule alpha 4) (Alpha 4 protein) CNOT10 3p22.3 CCR4-NOT transcription complex, subunit 10 LOC389101 3 similar to 60S ribosomal protein L23a LOC389102 3 similar to YPLR6490 LOC131405 3p22.3 similar to abnormal cell LINeage LIN-41, heterochronic gene; Drosophila dappled/ vertebrate TRipartite Motif protein related; B-box zinc finger, Filamin and NHL repeat containing protein (123.8 kD) (lin-41) CCR4 3p24 chemokine (C-C motif) receptor 4 LOC389103 3 LOC389103 GLB1 3p21.33 galactosidase, beta 1 CRTAP 3p22 cartilage associated protein KIAA0527 3p22.3 KIAA0527 protein FBXL2 3p24.3 F-box and leucine-rich repeat protein 2 UBP1 3p22.2 upstream binding protein 1 (LBP-1a) CLASP2 3p24.2-p21.33 cytoplasmic linker associated protein 2 LOC389104 3 similar to CLIP-associating protein 1; multiple asters 1 PDCD6IP 3p22.3 programmed cell death 6 interacting protein LOC285307 3p22.2 hypothetical LOC285307 FECHP 3p23-p22 ferrochelatase pseudogene LOC389105 3 LOC389105
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    38 splicing 7289 165 1506 - 2007 17342765
    - - 6733 - 1294 - 2007 17342765
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Hearing/Equilibriumearinnercochlea highly
    Lymphoid/Immunelymph node   highly
    Nervousbrain   highly
    Visualeye   highly
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • four heat repeats
  • microtubule-binding domain of CLASP2 (which is homologous between all CLASPs)
  • the N-terminal dis1/TOG domain of CLASP2alpha (which is homologous between alpha isoforms) possess actin-binding activity
  • secondary structure
  • two TOG domains of CLASP2 consisting of six HEAT repeats, which are similar to the canonical paddle-like tubulin-binding TOG domains, but have arched conformations
  • HOMOLOGY
    interspecies ortholog to Clasp2, Rattus norvegicus
    ortholog to Clasp2, Mus musculus
    ortholog to clasp2, Danio rerio
    ortholog to CLASP2, Pan troglodytes
    intraspecies paralog to CLASP1
    Homologene
    FAMILY
  • CLASP family
  • CATEGORY regulatory , structural protein
    SUBCELLULAR LOCALIZATION     plasma membrane
        intracellular
    intracellular,cytoplasm,organelle,membrane
    intracellular,cytoplasm,organelle,Golgi
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,centromere
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • colocalizing with CLIPs at microtubule distal ends
  • during mitosis, it localizes to centrosomes, the mitotic spindle, centrosomeres and spindle midzone
  • basic FUNCTION
  • orientation of stabilized microtubules and attachment to kinetochore
  • involved in local regulation of microtubule dynamics
  • playing a role in centrosome separation, organization and maintenance of the bipolar mitotic spindle
  • cooperate with CLASP1 to ensure mitotic fidelity by regulating spindle and kinetochore function
  • required for mitotic spindle organization and kinetochore alignment
  • function as actin/microtubule crosslinkers in interphase cells and facilitate recognition of actin filaments by the plus ends of growing microtubules at the initial stages of actin-microtubule interaction
  • essential for Golgi-associated microtubule nucleation and directional migration
  • CLASP2 and NEK1 proteins are present in a centrosomal complex and participate in cell cycle and cell division mechanisms
  • role in insulin action, where CLASP2 directs the delivery of SLC2A4 to cell cortex landing zones important for insulin action
  • microtubule plus-end tracking protein CLASP2 is a key regulator of axon and dendrite outgrowth that leads to functional alterations in synaptic activity and formation
  • during mitosis the kinetochore (KT)-microtubule (MT)-associated protein CLASP2 is progressively and distinctively phosphorylated by CDK1 and PLK1 kinases, concomitant with the establishment of KT-MT attachments
  • involvement of the microtubule plus-end tracking protein CLASP2 in cytoskeleton-related mechanisms underlying neuronal polarity and interplay between microtubule stabilization and synapse formation and activity
  • CLASP2-mediated stabilization of microtubules is required for proper attachment, homing, and maintenance of hematopoietic stem cells, and this is necessary to sustain MPL transcription
  • both GSK3B-dependent phosphorylation and the level of CLASP2 play a role in the maintenance of CHRNA1 cluster size through the regulated capture and release of Microtubule plus-ends
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • AKAP11 serves as a platform for the assembly of a CLASP2-IQGAP sub-complex
  • INTERACTION
    DNA
    RNA
    small molecule
    protein
  • CLIP2 and CLIP1, binding to microtubules and cooperate to direct the microtubule network and regulating cellular asymmetry
  • association with the kinetochores relies on its C-terminal domain, but is independent of microtubules or association with CLIP1
  • MAPRE1
  • estrogen receptor binding CpG island, EB1
  • CLASP2 interaction with IQGAP1 (interacts with IQGAP1 preferentially when calcium levels are increased)
  • the cascade linking AGRN to CLASP2-mediated microtubule capturing at the synaptic membrane is essential for the maintenance of a normal neuromuscular phenotype
  • role for the scaffolding protein EPB41 in locally controlling CLASP2 behavior, CLASP2 cortical platform turnover and GSK3 activity, enabling correct microtubule organization and dynamics essential for cell polarity
  • interacts with CTNND1 and governs microtubule dynamics at adherens junctions
  • functional relationship between ABL1 and CLASP2 is conserved and provides a means to control the CLASP2 association with the cytoskeleton
  • CLASP2 directly interacted with PARD3 and was phosphorylated by PRKCI
  • transport system, organized by AGRN through PI3 kinase, GSK3B, CLASP2, and PHLDB2, for precise delivery of CHRNA1 vesicles from the subsynaptic nuclei to the overlying synaptic membrane
  • cell & other
    REGULATION
    Phosphorylated by CDK1, PLK1
    ASSOCIATED DISORDERS
    corresponding disease(s)
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS
  • primary fibroblasts from Clasp2 knockout mice show numerous spindle and chromosome segregation defects that can be partially rescued by ectopic expression of Clasp1 or Clasp2