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FLASH GENE
Symbol CKAP5 contributors: mct/pgu - updated : 02-12-2015
HGNC name cytoskeleton associated protein 5
HGNC id 28959
Location 11p11.2      Physical location : 46.765.083 - 46.867.843
Synonym name
  • microtubule-associated colonic and hepatic tumor over-expressed
  • KIAA0097 gene product
  • Ch-TOG protein
  • colonic and hepatic tumor overexpressed gene
  • Synonym symbol(s) KIAA0097, CHTOG, FLJ35359, TOG, FLJ35359, TOGp, XMAP215, ch-TOG
    DNA
    TYPE functioning gene
    STRUCTURE 102.76 kb     43 Exon(s)
    MAPPING cloned Y linked N status provisional
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    44 - 6714 225 2032 - 2007 17376965
    isoform a
    43 - 6534 218 1972 - 2007 17376965
    isoform b
    EXPRESSION
    Type ubiquitous
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Cardiovascularheart    
    Digestiveliver    
    Endocrinethyroid    
    Hearing/Equilibriumear    
    Nervousbrainmidbrainsubstantia nigra  
    Reproductivefemale systembreastmammary gland  
    Respiratorylung    
    tissue
    SystemTissueTissue level 1Tissue level 2LevelPubmedSpeciesStageRna symbol
    Muscularstriatumskeletal  
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    physiological period pregnancy
    Text placenta,embryonic stem cells
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a microtubule-binding domain in its N-terminal half, with most-N-terminal TOG domain (TOG1) catalyzing subunit incorporation, whereas TOG2 helps to maintain attachment to the microtubule end
  • nine HEAT repeats
  • a KXGS motif for binding tubulin dimers in its C-terminal half
  • HOMOLOGY
    interspecies ortholog to murine Ckap5
    homolog to xenopus Xmap215
    Homologene
    FAMILY
  • TOG/XMAP 215 family
  • XMAP215 MAP family
  • CATEGORY regulatory , protooncogene
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytoskeleton,microtubule,centrosome
    intracellular,cytoplasm,cytoskeleton,microtubule,mitotic spindle
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • primarily to the spindle pole body (spb) and to a lesser extent along spindle microtubules
  • localizes to the centrosome
  • localization at the microtubule plus end and centrosomes, and kinetochores
  • basic FUNCTION
  • may be playing a role in the attachment, organization, and/or dynamics of microtubule ends at the spindle pole body
  • mediating the association of hnRNP A2-positive granules with microtubules during transport and/or localization
  • plays a major role in organizing spindle poles, whereas its role in stabilizing spindle microtubules is minor and, at least in part, mediated via interaction with TACC3
  • processive polymerase that catalyzes numerous rounds of tubulin subunit addition while at the microtubule end and argue against the tubulin shuttle model
  • displays an elevated expression pattern in tumor cells, and is thought to enhance microtubule polymerization and nucleation
  • microtubule-associated protein that binds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2
  • is necessary for myelin basic protein expression in oligodendrocytes
  • ensures spindle bipolarity by counteracting the activity of the microtubule-depolymerizing kinesin KIF2C
  • protects kinetochore microtubules from depolymerization by KIF2C and plays an essential role in centrosomal microtubule assembly, a function independent of KIF2C activity
  • potentially required for chromosome motility as a downstream consequence of reduced microtubule dynamics and/or density
  • is a microtubule plus-end binding protein that alters microtubule dynamics by promoting microtubule growth
  • a TACC3/CKAP5/clathrin complex stabilises kinetochore fibres by inter-microtubule bridging
  • MAPRE1 and microtubule polymerase CKAP5-act together to strongly promote microtubule growth to cellular rates
  • is one of several proteins capable of coupling to the microtubule ends under load
  • dynamically links to the ends of growing and shrinking microtubules
  • accelerates microtubule growth, and it retains its polymerase activity when coupled to a bead
  • CKAP5 is likely important in oocyte maturation and early embryo development, and CKAP5 might work together with CLTC in oocyte maturation
  • CELLULAR PROCESS
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
    INTERACTION
    DNA
    RNA
    small molecule
    protein
  • interacting with TACC1
  • binding tubulin
  • interacting with AURKA (involved in the regulation of CKAP5 and KIF2C localization)
  • TACC3, is a centrosomal protein that interacts with CKAP5
  • ILK performs its centrosome clustering activity in a focal adhesion-independent, but centrosome-dependent, manner through the microtubule regulating proteins TACC3 and CKAP5
  • interacting with SLAIN2 (N-terminal part of SLAIN2 interacted with CKAP5)(Pubmed 21646404)
  • binds preferentially to the microtubule plus end and follows the growth and shrinkage of the microtubule tip, catalyzing microtubule growth
  • clathrin promotes centrosome maturation by stabilizing the microtubule-binding protein CKAP5, defining a novel role for the clathrin-CKAP5-TACC3 complex
  • SLAIN1, SLAIN2 promote persistent microtubule growth by recruiting the microtubule polymerase CKAP5 to microtubule plus-ends
  • C-terminal TACC domain of TACC3 and a C-terminal fragment adjacent to the TOG domains of CKAP5 mediate the interaction between TACC and CKAP5, interaction required for spindle assembly and microtubule dynamics during mitotic cell division
  • cell & other
    REGULATION
    ASSOCIATED DISORDERS
    ANIMAL & CELL MODELS