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FLASH GENE
Symbol CENPE contributors: mct - updated : 27-09-2016
HGNC name centromere protein E, 312kDa
HGNC id 1856
Corresponding disease
MCPH13 microcephaly 13, primary
Location 4q24      Physical location : 104.026.962 - 104.119.566
Synonym name
  • kinesin family member 10
  • centromere autoantigen E (312kD)
  • kinesin-related protein CENPE
  • protein phosphatase 1, regulatory subunit 61
  • Synonym symbol(s) KIF10, CENP-E, MCPH13, PPP1R61
    DNA
    TYPE functioning gene
    STRUCTURE 92.60 kb     49 Exon(s)
    10 Kb 5' upstream gene genomic sequence study
    MAPPING cloned Y linked N status provisional
    Physical map
    PPP3CA 4q21-q24 protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha) LOC90024 4q24 hypothetical LOC90024 BANK1 4q23 B-cell scaffold protein with ankyrin repeats 1 SLC39A8 4q22-q24 solute carrier family 39 (zinc transporter), member 8 NFKB1 4q24 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (p105) MANBA 4q22-q25 mannosidase, beta A, lysosomal UBE2D3 4q22.2 ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast) LOC150159 4q24 CG10806-like LOC133308 4q24 hypothetical protein BC009732 DHRS6 4q24 dehydrogenase/reductase (SDR family) member 6 CENPE 4q24-q25 centromere protein E, 312kDa LOC391678 4 similar to DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD/H box-3; helicase like protein 2; CAP-Rf TACR3 4q25 tachykinin receptor 3 IDAX 4q22-q24 tachykinin receptor 3 LOC391679 4 similar to 60S ribosomal protein L6 (TAX-responsive enhancer element binding protein 107) (TAXREB107) (Neoplasm-related protein C140) FLJ20032 4q24 hypothetical protein FLJ20032 KIAA1546 4q24 KIAA1546 protein PPA2 4q25 inorganic pyrophosphatase 2 LOC389214 4 similar to Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu)
    RNA
    TRANSCRIPTS type messenger
    identificationnb exonstypebpproduct
    ProteinkDaAAspecific expressionYearPubmed
    49 - 8630 316.4 2701 - 2008 18374647
    47 - 8267 - 2580 - 2008 18374647
    EXPRESSION
    Type widely
       expressed in (based on citations)
    organ(s)
    SystemOrgan level 1Organ level 2Organ level 3Organ level 4LevelPubmedSpeciesStageRna symbol
    Digestiveliver    
    Endocrineadrenal gland   highly
    Lymphoid/Immunelymph node   highly
    Nervousbrain    
    Reproductivemale systemprostate   
    cell lineage
    cell lines
    fluid/secretion
    at STAGE
    cell cycle     cell cycle, G2, M, checkpoint, G2M
    PROTEIN
    PHYSICAL PROPERTIES
    STRUCTURE
    motifs/domains
  • a N terminal motor domain,
  • a conserved residue (T422) close to the motor domain phosphorylated by AURKA and AURKB, which is essential to promote the congression of polar chromosomes and dephosphorylation of this site and is required for the stable biorientation of these kinetochores
  • an ATP binding site
  • a C-terminal tail phsphorylated by CDK1, MAPK, or MPS1 previously proposed either to regulate CENPE motor activity prior to its binding to kinetochores, or inhibit a microtubule binding site in the tail that may link anti-parallel, midzone microtubules in anaphase
  • conjugated LipoP
    HOMOLOGY
    interspecies homolog to murine Cenpe
    Homologene
    FAMILY
  • kinesin-like protein family
  • kinesin-7 family
  • CATEGORY motor/contractile
    SUBCELLULAR LOCALIZATION     intracellular
    intracellular,cytoplasm,cytosolic
    intracellular,cytoplasm,cytoskeleton,microtubule
    intracellular,nucleus,chromatin/chromosome,kinetochore
    text
  • may be accumulated at kinetochores in unaligned chromosomes in PSRC1-depleted cells to perform its gliding function
  • mitotic kinesin whose expression is up-regulated during G2 and M phases of the cell cycle, and it localizes to kinetochores from early prometaphase through metaphase moving to the antiparallel midzone MTs at anaphase
  • basic FUNCTION
  • kinesin-like motor protein that accumulates in the G2 phase of the cell cycle
  • required for the metaphase-anaphase transition in mitosis and meiosis II
  • may be one of the motors responsible for mammalian chromosome movement and/or spindle elongation
  • possess SUMO-2/3 polymeric chain-binding activity essential for kinetochore localization
  • implicated in powering chromosome congression by transporting mono-oriented chromosomes to the spindle equator along mature kinetochore fibers of already bioriented chromosomes
  • involved in proliferation, and aggressiveness of prolactin pituitary tumors
  • functioning as a motor that carries chromosomes toward the metaphase plate, in a manner very similar to how kinesin-1 transports vesicles
  • its enzymatic cycle is characterized by rapid ATP binding, multiple enzymatic turnovers per diffusive
  • encounter, and gating of nucleotide binding
  • functions by actively transporting chromosomes along kinetochore fibers (Rosenfeld 2009)
  • kinesin-like motor protein required for efficient capture and attachment of spindle microtubules by kinetochores, a necessary step in chromosome alignment in the metaphase plate
  • KIF2C and CENPE are involved in PSRC1-mediated chromosome congression
  • SKAP cooperates with CENPE to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation
  • processive kinesin that is required for chromosome congression and stable chromosome biorientation
  • exhibits unusually slow microtubule collision coupled with slow ADP release, and this novel ATPase cycle may favor CENPE binding of stable kinetochore microtubules over dynamic microtubules
  • is vital for maintaining cell cycle and checkpoint signal mechanisms are vital for recruitment process of other essential kinetochore proteins
  • lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENPE and KIF2C
  • core kinetochore component functioning to mediate chromosome congression initially of misaligned chromosomes and in subsequent spindle microtubule capture during mitosis
  • is a highly elongated kinesin that transports pole-proximal chromosomes during congression in prometaphase
  • unexpected role of CENPE elongated stalk in ensuring stability of kinetochore-microtubule attachments during chromosome congression and segregation
  • CENPE suppresses chromosome congression, probably by tethering kinetochores to short, unstable microtubules, and works in congression only when microtubules are stabilized
  • differential contributions of KIF22 and CENPE in chromosome congression in physiological conditions where stabilized microtubules are becoming increased
  • is a microtubule plus-end-directed kinetochore motor required for congression of pole-proximal chromosomes
  • CELLULAR PROCESS cell cycle,division,meiosis
    cell cycle, division, mitosis
    cell cycle, checkpoint
    nucleotide, replication
    PHYSIOLOGICAL PROCESS
    PATHWAY
    metabolism
    signaling
    a component
  • constituent of the outer kinetochore
  • central component in the mitotic checkpoint, modulating signaling activity in a microtubule dependent manner
  • targets to the midbody prior to SKP1 and the midbody localization of CENPE becomes diminished as SKP1 arrives at the midbody
  • INTERACTION
    DNA
    RNA
    small molecule nucleotide,
  • ATP
  • protein
  • binding BUB1B
  • weak interaction between the ZW10/KNTC1/ZWILCH complex and the kinesin-like kinetochore component CENPE
  • KIF18A physically interacted with CENPE and BUB1B during mitosis
  • interacting with CENPF
  • PARP1 interaction with CENPB, CENPE, and CENPF during mitosis and apoptosis
  • N-terminal 260 AAs of FANCA were found to be necessary and sufficient for the interaction with the C-terminus of CENPE
  • interaction of CENPE with FANCA (CENPE and FANCA may play important roles in the functional regulation of the mitotic checkpoint signal pathway)
  • binding of PPP1CC to CENPE required for the stable attachmentof these chromosomes
  • contributes to chromosome alignment at the metaphase plate by localizing CENPE at kinetochore regions
  • CENPE-dependent BUB1B autophosphorylation in response to spindle microtubule capture by CENPE is important for kinetochore function in achieving accurate chromosome segregation
  • lateral to end-on conversion of chromosome-microtubule attachment requires kinesins CENPE and KIF2C
  • CENPQ targets PLK1 to kinetochores and is also required for the recruitment of CENPE to kinetochores
  • CENPE-driven chromosome congression is guided by microtubule detyrosination
  • TRAPPC12 interacts with CENPE, and depletion of TRAPPC12 prevented the recruitment of CENPE to the kinetochore
  • CTCF interacts with the centromeric protein CENPE, CTCF helps recruit CENPE to the centromere during mitosis and it may do so through a structure stabilized by the CTCF/CENPE complex
  • CHAMP1 regulates localization of CENPE and CENPF to kinetochores
  • cell & other
  • microtubule associated kinesin
  • REGULATION
    Other modified specifically by SUMO-2/3, possessing SUMO-2/3 polymeric chain-binding activity essential for kinetochore localization
    plus-end directed motor properties of CENPE (and binding of PPP1CC) are altered by a gradient of Aurora kinase activity emanating from the spindle poles
    ASSOCIATED DISORDERS
    corresponding disease(s) MCPH13
    Susceptibility
    Variant & Polymorphism
    Candidate gene
    Marker
    Therapy target
    ANIMAL & CELL MODELS